Report on: + IDNOSPACE +

Donor/Replicate	All	Productive	Unique Productive	Unproductive	Unique Unproductive
IDNOSPACE	22433	11147 (50%)	11147 (50%)	2794 (12%)	2794 (12%)
IDNOSPACE_1	22433	11147 (50%)	11147 (50%)	2794 (12%)	2794 (12%)

+ + + + + + + + + + + + + + + +

+ +

Gene frequencies tab

+ +

This tab gives +information on the frequency of V, D and J gene usage.

+ +

Graphs

+ +

Distribution of +V gene families

+ +

Bar graph showing the frequency of V gene family usage +in all uploaded donors.

+ +

Relative +frequency of V gene usage

+ +

Bar graph showing the frequency of V genes in all +uploaded donors. The order of the V genes on the x-axis can be altered by +altering the “Order of V(D)J genes +in graphs” filter on the main page of the Immune repertoire pipeline.

+ +

Relative +frequency of D gene usage

+ +

Bar graph showing the frequency of D genes in all uploaded donors. The +order of the D genes on the x-axis can be altered by altering the “Order of V(D)J genes in graphs” filter on +the main page of the Immune repertoire pipeline.

+ +

Relative +frequency of J gene usage

+ +

Bar graph showing the frequency of J genes in all uploaded donors. The +order of the J genes on the x-axis can be altered by altering the “Order of V(D)J genes in graphs” filter on +the main page of the Immune repertoire pipeline.

+ +

D +reading frame

+ +

Bar graph visualizing the frequency by which each D reading frame is +used in each donor.

+ +

+ + + + +

+ + + +

Donor	Median CDR3 Length
IDNOSPACE	13

+ + + + + + + + + + + +

+ +

CDR3 characteristics tab

+ +

This tab gives information on the +different characteristics of the CDR3.

+ +

Graphs

+ +

length distribution of CDR3

+ +

Graph visualizing for each donor the +distribution of the CDR3 length in amino acids between all sequences.

+ +

Amino acid composition of CDR3

+ +

Bar graph visualizing for each donor how +frequent each amino acid is used in the CDR3 of all sequences. The red colour +in the background indicate positively charged amino acids, and the blue colour +in the background indicate negatively charged amino acids. The amino acids are +ordered from hydrophilic (left) to hydrophobic (right).

+ +

Table

+ +

Table showing for each donor the median +CDR3 length.

+ +

+ + + + +

+ +

+ + + + + + + + + + + +

+ +

In this tab +heatmaps are provided showing the frequency of different combinations V and D, +D and J and V and J genes according together. For each donor a subtab with all +three heatmaps is provided.

+ +

+ + + + +

+ +

ID	Include
IDNOSPACE

+ + + + + + + + + + + +

+ +

The tab allows +you to compare the VD, VJ and DJ heatmaps of multiple donors to each other.

+ +

By ticking the include box of a donor, the three +heatmaps of this donor is visualized underneath eachother. By clicking the +include box of multiple replicates the heatmaps of these replicates are visualized +next to each other allowing easy comparison of heatmaps.

+ +

+ + + + +

+ +

V-J

+ + + + + + + + + + + +

+ +

Martin Krzywinski, Jacqueline Schein, İnanç +Birol, Joseph Connors, Randy Gascoyne, Doug Horsman, Steven J. Jones and Marco +A. Marra (2009). Circos: An information aesthetic for comparative genomics. In Genome +Research 10.1101/gr.092759.109.

+ +

Circos plot showing the V-D, V-J, and D-J gen usage. +The thicker the line the more frequent this gene combination is used.

+ +

+ + + + +

Unique rearrangements with a V, D and J gene assigned

+ + +

Productive mean
Donor	Number of sequences	V.DEL	P1	N1	P2	DEL.D	D.DEL	P3	N2	P4	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	11049	1.5	0.2	5.3	0.1	7.2	5.5	0.1	5.6	0.1	6.8	21	10.9	0.4	13

+ + +

Unproductive mean
Donor	Number of sequences	V.DEL	P1	N1	P2	DEL.D	D.DEL	P3	N2	P4	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	2705	2	0.2	7.8	0.1	7.1	4.5	0	10	0	12.1	25.7	17.8	0.4	-

+ + +

Productive median
Donor	Number of sequences	V.DEL	P1	N1	P2	DEL.D	D.DEL	P3	N2	P4	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	11049	1	0	4	0	6	4	0	5	0	6	21	10	0	13

+ + +

Unproductive median
Donor	Number of sequences	V.DEL	P1	N1	P2	DEL.D	D.DEL	P3	N2	P4	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	2705	1	0	6	0	6	3	0	10	0	15	26	17	0	-

Unique rearrangements with only a V and J gene assigned

+ + +

Productive mean
Donor	Number of sequences	V.DEL	P1	N	P2	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	98	1.1	0.3	3.2	0	4.7	1.1	3.2	0.1	8

+ + +

Unproductive mean
Donor	Number of sequences	V.DEL	P1	N	P2	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	89	1	0	3	0	0	0	3	0	-

+ + +

Productive median
Donor	Number of sequences	V.DEL	P1	N	P2	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	98	1	0	3	0	2.5	0	3	0	8

+ + +

Unproductive median
Donor	Number of sequences	V.DEL	P1	N	P2	DEL.J	Total.Del	Total.N	Total.P	CDR3.Length
IDNOSPACE	89	1	0	3	0	0	0	3	0	-

+ + + + + + + + + + + + + + + +

+ +

Junction +analysis tab

+ +

Tables:

+ +

The unique +rearrangements are separated in sequences that contain both a +V, D and J gene and sequences that contain only a V and J gene.

+ +

Productive mean +tables

+ +

Reports +for each donor the number of productive sequences and the mean number of +deletions, N-nucleotides and P-nucleotides. +For explanation about the different deletions and N and P nucleotides see +figure above.

+ +

Unproductive +mean table

+ +

Reports +for each donor the number of unproductive sequences and the mean number of +deletions, N-nucleotides and P-nucleotides. +For explanation about the different deletions and N and P nucleotides see +figure above.

+ +

Productive +median table

+ +

Reports +for each donor the number of productive sequences and the median number of +deletions, N-nucleotides and P-nucleotides. +For explanation about the different deletions and N and P nucleotides see +figure above.

+ +

Unproductive +median table

+ +

Reports +for each donor the number of unproductive sequences and the median number of +deletions, N-nucleotides and P-nucleotides. +For explanation about the different deletions and N and P nucleotides see +figure above.

+ +

+ + + + +

+ + + + + + + + + + + + + + + + + + +

Description	Link
All outputs below in a zip file	Download
The filtered dataset	Download
Gene frequencies
The dataset used to generate the distribution of V gene families graph	Download
The dataset used to generate the relative frequency of V gene usage graph	Download
The dataset used to generate the relative frequency of J gene usage graph	Download
The dataset used to generate the relative frequency of the D reading frame graph	Download
CDR3 Characteristics
The dataset used to generate the CDR3 length frequency graph	Download
The dataset used to generate the Amino Acid Composition in the CDR3 graph	Download
Heatmaps
The data used to generate the VJ heatmap for IDNOSPACE.	Download
Circos
The data used to generate the VJ Circos plots for IDNOSPACE.	Download
Clonality
The dataset used to calculate clonality score (Unique based on clonaltype, none)	Download

+ + + + + + + + + + + +

+ +

Downloads +tab

+ +

Description

+ +

The +filtered dataset: Allows you to download information on V D and J gene usage, +the junctions and the amino acid sequence of the sequences that passed the chosen +filter settings. Note: this file contains only 1 sequences of a replicate +sequence.

+ +

Gene +frequencies

+ +

The dataset used to generate the distribution +of V gene families graph: Downloads a table with information used to generate +the distribution of V gene families graph.

+ +

The dataset used to generate the +distribution of D gene families graph: Downloads a table with information used +to generate the distribution of D gene families graph.

+ +

The +dataset used to generate the relative frequency of V gene usage graph: +Downloads the data used to generate the relative frequency of V gene usage +graph. In addition information on the chromosomal order of the V genes can be +found.

+ +

The +dataset used to generate the relative frequency of D gene usage graph: +Downloads the data used to generate the relative frequency of D gene usage +graph. In addition information on the chromosomal order of the D genes can be +found.

+ +

The +dataset used to generate the relative frequency of J gene usage graph: Downloads +the data used to generate the relative frequency of J gene usage graph. In +addition information on the chromosomal order of the J genes can be found.

+ +

The dataset used to generate the relative frequency of the D +reading frame graph: Downloads +the data used to generate the relative frequency of the D reading frame.

+ +

CDR3 characteristics

+ +

The dataset used to generate the CDR3 +length frequency graph: Downloads a table with information used for the +length distribution of CDR3 plot.

+ +

The +dataset used to generate the Amino Acid Composition in the CDR3 graph: +Downloads the frequency of the different amino acids in the CDR3 of all +filtered sequences. This data set is used for the generation of the Amino Acid +Composition in the CDR3 graph. In addition the order of amino acids based on hydrophobicity +in included.

+ +

Heatmaps

+ +

The +data used to generate the VD heatmap for “donor name”: +Downloads the data set used for the generation of the VD heatmap. For each +uploaded donor a separate download is generated.

+ +

The +data used to generate the VJ heatmap for “donor name”: +Downloads the data set used for the generation of the VJ heatmap. For each +uploaded donor a separate download is generated.

+ +

The +data used to generate the DJ heatmap for “sample name”: +Downloads the data set used for the generation of the DJ heatmap. For each +uploaded donor a separate download is generated.

+ +

Circos

+ +

The +data used to generate the VD circus plots for “donor name”: +Downloads the data set used for the generation of the VD heatmap. For each +uploaded donor a separate download is generated.

+ +

The +data used to generate the VJ circus plots for “donor name”: +Downloads the data set used for the generation of the VJ heatmap. For each +uploaded donor a separate download is generated.

+ +

The +data used to generate the DJ circus plots for “sample name”: +Downloads the data set used for the generation of the DJ heatmap. For each +uploaded donor a separate download is generated.

+ +

Clonality

+ +

The +dataset used to calculate clonality score: Allows you to +download information on V D and J gene usage, the junctions and the amino acid +sequence of the sequences that passed the chosen filter settings. Note: this +file contains all the replicate sequences form the different input files.

+ +

Sequences +that are present in more than one replicate: Allows you to +download a table containing all the sequences that were present in more than +one replicate. It provides the concidence type, clonaltype, sequence name, +replicate number, and the nucleotide sequence

+ +

+ + + + +

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