annotate pattern_plots.r @ 44:716622f5bb4f draft

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1 library(ggplot2)
2 library(reshape2)
3 library(scales)
4 
5 args <- commandArgs(trailingOnly = TRUE)
6 
7 input.file = args[1] #the data that's get turned into the "SHM overview" table in the html report "data_sum.txt"
8 
9 plot1.path = args[2]
10 plot1.png = paste(plot1.path, ".png", sep="")
11 plot1.txt = paste(plot1.path, ".txt", sep="")
12 plot1.pdf = paste(plot1.path, ".pdf", sep="")
13 
14 plot2.path = args[3]
15 plot2.png = paste(plot2.path, ".png", sep="")
16 plot2.txt = paste(plot2.path, ".txt", sep="")
17 plot2.pdf = paste(plot2.path, ".pdf", sep="")
18 
19 plot3.path = args[4]
20 plot3.png = paste(plot3.path, ".png", sep="")
21 plot3.txt = paste(plot3.path, ".txt", sep="")
22 plot3.pdf = paste(plot3.path, ".pdf", sep="")
23 
24 clean.output = args[5]
25 
26 dat = read.table(input.file, header=F, sep=",", quote="", stringsAsFactors=F, fill=T, row.names=1)
27 
28 classes = c("IGA", "IGA1", "IGA2", "IGG", "IGG1", "IGG2", "IGG3", "IGG4", "IGM", "IGE")
29 xyz = c("x", "y", "z")
30 new.names = c(paste(rep(classes, each=3), xyz, sep="."), paste("un", xyz, sep="."), paste("all", xyz, sep="."))
31 
32 names(dat) = new.names
33 
34 clean.dat = dat
35 clean.dat = clean.dat[,c(paste(rep(classes, each=3), xyz, sep="."), paste("all", xyz, sep="."), paste("un", xyz, sep="."))]
36 
37 write.table(clean.dat, clean.output, quote=F, sep="\t", na="", row.names=T, col.names=NA)
38 
39 dat["RGYW.WRCY",] = colSums(dat[c(13,14),], na.rm=T)
40 dat["TW.WA",] = colSums(dat[c(15,16),], na.rm=T)
41 
42 data1 = dat[c("RGYW.WRCY", "TW.WA"),]
43 
44 data1 = data1[,names(data1)[grepl(".z", names(data1))]]
45 names(data1) = gsub("\\..*", "", names(data1))
46 
47 data1 = melt(t(data1))
48 
49 names(data1) = c("Class", "Type", "value")
50 
51 chk = is.na(data1$value)
52 if(any(chk)){
53 	data1[chk, "value"] = 0
54 }
55 
56 data1 = data1[order(data1$Type),]
57 
58 write.table(data1, plot1.txt, quote=F, sep="\t", na="", row.names=F, col.names=T)
59 
60 p = ggplot(data1, aes(Class, value)) + geom_bar(aes(fill=Type), stat="identity", position="dodge", colour = "black") + ylab("% of mutations") + guides(fill=guide_legend(title=NULL)) + ggtitle("Percentage of mutations in AID and pol eta motives")
61 p = p + theme(panel.background = element_rect(fill = "white", colour="black"),text = element_text(size=15, colour="black"), axis.text.x = element_text(angle = 45, hjust = 1)) + scale_fill_manual(values=c("RGYW.WRCY" = "white", "TW.WA" = "blue4"))
62 #p = p + scale_colour_manual(values=c("RGYW.WRCY" = "black", "TW.WA" = "blue4"))
63 png(filename=plot1.png, width=510, height=300)
64 print(p)
65 dev.off()
66 
67 ggsave(plot1.pdf, p)
68 
69 data2 = dat[c(1, 5:8),]
70 
71 data2 = data2[,names(data2)[grepl("\\.x", names(data2))]]
72 names(data2) = gsub(".x", "", names(data2))
73 
74 data2["A/T",] = dat["Targeting of A T (%)",names(dat)[grepl("\\.z", names(dat))]]
75 
76 data2["G/C transitions",] = round(data2["Transitions at G C (%)",] / data2["Number of Mutations (%)",] * 100, 1)
77 
78 data2["mutation.at.gc",] = dat["Transitions at G C (%)",names(dat)[grepl("\\.y", names(dat))]]
79 data2["G/C transversions",] = round((data2["mutation.at.gc",] - data2["Transitions at G C (%)",]) / data2["Number of Mutations (%)",] * 100, 1)
80 
81 data2["G/C transversions",is.nan(unlist(data2["G/C transversions",]))] = 0
82 data2["G/C transversions",is.infinite(unlist(data2["G/C transversions",]))] = 0
83 data2["G/C transitions",is.nan(unlist(data2["G/C transitions",]))] = 0
84 data2["G/C transitions",is.infinite(unlist(data2["G/C transitions",]))] = 0
85 
86 data2 = melt(t(data2[c("A/T","G/C transitions","G/C transversions"),]))
87 
88 names(data2) = c("Class", "Type", "value")
89 
90 chk = is.na(data2$value)
91 if(any(chk)){
92 	data2[chk, "value"] = 0
93 }
94 
95 data2 = data2[order(data2$Type),]
96 
97 write.table(data2, plot2.txt, quote=F, sep="\t", na="", row.names=F, col.names=T)
98 
99 p = ggplot(data2, aes(x=Class, y=value, fill=Type)) + geom_bar(position="fill", stat="identity", colour = "black") + scale_y_continuous(labels=percent_format()) + guides(fill=guide_legend(title=NULL)) + ylab("% of mutations") + ggtitle("Relative mutation patterns")
00 p = p + theme(panel.background = element_rect(fill = "white", colour="black"), text = element_text(size=15, colour="black"), axis.text.x = element_text(angle = 45, hjust = 1)) + scale_fill_manual(values=c("A/T" = "blue4", "G/C transversions" = "gray74", "G/C transitions" = "white"))
01 #p = p + scale_colour_manual(values=c("A/T" = "blue4", "G/C transversions" = "gray74", "G/C transitions" = "black"))
02 png(filename=plot2.png, width=480, height=300)
03 print(p)
04 dev.off()
05 
06 ggsave(plot2.pdf, p)
07 
08 data3 = dat[c(5, 6, 8, 17:20),]
09 data3 = data3[,names(data3)[grepl("\\.x", names(data3))]]
10 names(data3) = gsub(".x", "", names(data3))
11 
12 data3["G/C transitions",] = round(data3["Transitions at G C (%)",] / (data3["C",] + data3["G",]) * 100, 1)
13 
14 data3["G/C transversions",] = round((data3["Targeting of G C (%)",] - data3["Transitions at G C (%)",]) / (data3["C",] + data3["G",]) * 100, 1)
15 
16 data3["A/T",] = round(data3["Targeting of A T (%)",] / (data3["A",] + data3["T",]) * 100, 1)
17 
18 data3["G/C transitions",is.nan(unlist(data3["G/C transitions",]))] = 0
19 data3["G/C transitions",is.infinite(unlist(data3["G/C transitions",]))] = 0
20 
21 data3["G/C transversions",is.nan(unlist(data3["G/C transversions",]))] = 0
22 data3["G/C transversions",is.infinite(unlist(data3["G/C transversions",]))] = 0
23 
24 data3["A/T",is.nan(unlist(data3["A/T",]))] = 0
25 data3["A/T",is.infinite(unlist(data3["A/T",]))] = 0
26 
27 data3 = melt(t(data3[8:10,]))
28 names(data3) = c("Class", "Type", "value")
29 
30 chk = is.na(data3$value)
31 if(any(chk)){
32 	data3[chk, "value"] = 0
33 }
34 
35 data3 = data3[order(data3$Type),]
36 
37 write.table(data3, plot3.txt, quote=F, sep="\t", na="", row.names=F, col.names=T)
38 
39 p = ggplot(data3, aes(Class, value)) + geom_bar(aes(fill=Type), stat="identity", position="dodge", colour = "black") + ylab("% of nucleotides") + guides(fill=guide_legend(title=NULL)) + ggtitle("Absolute mutation patterns")
40 p = p + theme(panel.background = element_rect(fill = "white", colour="black"), text = element_text(size=15, colour="black"), axis.text.x = element_text(angle = 45, hjust = 1)) + scale_fill_manual(values=c("A/T" = "blue4", "G/C transversions" = "gray74", "G/C transitions" = "white"))
41 #p = p + scale_colour_manual(values=c("A/T" = "blue4", "G/C transversions" = "gray74", "G/C transitions" = "black"))
42 png(filename=plot3.png, width=480, height=300)
43 print(p)
44 dev.off()
45 
46 ggsave(plot3.pdf, p)
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