Mercurial > repos > davidvanzessen > shm_csr
annotate shm_overview.htm @ 93:8fcf31272f6e draft
planemo upload commit a43893724cc769bed8a1f19a5b19ec1ba20cb63c
| author | rhpvorderman |
|---|---|
| date | Mon, 06 Mar 2023 11:36:32 +0000 |
| parents | 6809c63d9161 |
| children |
| rev | line source |
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| 67 | 1 <html> |
| 2 | |
| 3 <head> | |
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"planemo upload commit fd64827ff6e63df008f6f50ddb8576ad2b1dbb26"
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4 <meta http-equiv=Content-Type content="text/html; charset=UTF-8"> |
| 67 | 5 <meta name=Generator content="Microsoft Word 14 (filtered)"> |
| 6 <style> | |
| 7 <!-- | |
| 8 /* Font Definitions */ | |
| 9 @font-face | |
| 10 {font-family:Calibri; | |
| 11 panose-1:2 15 5 2 2 2 4 3 2 4;} | |
| 12 /* Style Definitions */ | |
| 13 p.MsoNormal, li.MsoNormal, div.MsoNormal | |
| 14 {margin-top:0in; | |
| 15 margin-right:0in; | |
| 16 margin-bottom:10.0pt; | |
| 17 margin-left:0in; | |
| 18 line-height:115%; | |
| 19 font-size:11.0pt; | |
| 20 font-family:"Calibri","sans-serif";} | |
| 21 .MsoChpDefault | |
| 22 {font-family:"Calibri","sans-serif";} | |
| 23 .MsoPapDefault | |
| 24 {margin-bottom:10.0pt; | |
| 25 line-height:115%;} | |
| 26 @page WordSection1 | |
| 27 {size:8.5in 11.0in; | |
| 28 margin:1.0in 1.0in 1.0in 1.0in;} | |
| 29 div.WordSection1 | |
| 30 {page:WordSection1;} | |
| 31 --> | |
| 32 </style> | |
| 33 | |
| 34 </head> | |
| 35 | |
| 36 <body lang=EN-US> | |
| 37 | |
| 38 <div class=WordSection1> | |
| 39 | |
| 40 <p class=MsoNormalCxSpFirst style='text-align:justify'><b><span lang=EN-GB | |
| 41 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Info | |
| 42 table</span></b></p> | |
| 43 | |
| 44 <p class=MsoNormalCxSpMiddle style='text-align:justify'><span lang=EN-GB | |
| 45 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>This | |
| 46 table contains information on different characteristics of SHM. For all | |
| 47 characteristics information can be found for all sequences or only sequences of | |
| 48 a certain (sub)class. All results are based on the sequences that passed the filter | |
| 49 settings chosen on the start page of the SHM & CSR pipeline and only | |
| 50 include details on the analysed region as determined by the setting of the | |
| 51 sequence starts at filter. All data in this table can be downloaded via the | |
|
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52 “downloads” tab.</span></p> |
| 67 | 53 |
| 54 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB | |
| 55 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Mutation | |
| 56 frequency:</span></u></p> | |
| 57 | |
| 58 <p class=MsoNormalCxSpMiddle style='text-align:justify'><a name="OLE_LINK83"></a><a | |
| 59 name="OLE_LINK82"></a><a name="OLE_LINK81"><span lang=EN-GB style='font-size: | |
| 60 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>These values | |
| 61 give information on the level of SHM. </span></a><a name="OLE_LINK22"></a><a | |
| 62 name="OLE_LINK21"></a><a name="OLE_LINK20"><span lang=EN-GB style='font-size: | |
| 63 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>More information | |
| 64 on the values found in healthy individuals of different ages can be found in </span></a><a | |
| 65 name="OLE_LINK15"></a><a name="OLE_LINK14"></a><a name="OLE_LINK13"><span | |
| 66 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>IJspeert | |
| 67 and van Schouwenburg et al, PMID: 27799928</span></a></p> | |
| 68 | |
| 69 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 70 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Number | |
| 71 of mutations:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height: | |
| 72 115%;font-family:"Times New Roman","serif"'> Shows the number of total | |
| 73 mutations / the number of sequenced bases (the % of mutated bases).</span></p> | |
| 74 | |
| 75 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 76 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Median | |
| 77 number of mutations:</span></i><span lang=EN-GB style='font-size:12.0pt; | |
| 78 line-height:115%;font-family:"Times New Roman","serif"'> Shows the median % of | |
| 79 SHM of all sequences.</span></p> | |
| 80 | |
| 81 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB | |
| 82 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Patterns | |
| 83 of SHM:</span></u></p> | |
| 84 | |
| 85 <p class=MsoNormalCxSpMiddle style='text-align:justify'><a name="OLE_LINK72"></a><a | |
| 86 name="OLE_LINK71"></a><a name="OLE_LINK70"><span lang=EN-GB style='font-size: | |
| 87 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>These values | |
| 88 give insights into the targeting and patterns of SHM. These values can give | |
| 89 insight into the repair pathways used to repair the U:G mismatches introduced | |
| 90 by AID. </span></a><a name="OLE_LINK40"></a><a name="OLE_LINK39"></a><a | |
| 91 name="OLE_LINK38"></a><a name="OLE_LINK60"><span lang=EN-GB style='font-size: | |
| 92 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>More information | |
| 93 on the values found in healthy individuals of different ages can be found in | |
| 94 IJspeert and van Schouwenburg et al, PMID: 27799928</span></a></p> | |
| 95 | |
| 96 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 97 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Transitions:</span></i><span | |
| 98 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 99 Shows the number of transition mutations / the number of total mutations (the | |
| 100 percentage of mutations that are transitions). Transition mutations are C>T, | |
| 101 T>C, A>G, G>A. </span></p> | |
| 102 | |
| 103 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 104 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Transversions:</span></i><span | |
| 105 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 106 Shows the number of transversion mutations / the number of total mutations (the | |
| 107 percentage of mutations that are transitions). Transversion mutations are | |
| 108 C>A, C>G, T>A, T>G, A>T, A>C, G>T, G>C.</span></p> | |
| 109 | |
| 110 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 111 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Transitions | |
| 112 at GC:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 113 font-family:"Times New Roman","serif"'> <a name="OLE_LINK2"></a><a | |
| 114 name="OLE_LINK1">Shows the number of transitions at GC locations (C>T, | |
| 115 G>A) / the total number of mutations at GC locations (the percentage of | |
| 116 mutations at GC locations that are transitions).</a></span></p> | |
| 117 | |
| 118 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 119 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Targeting | |
| 120 of GC:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 121 font-family:"Times New Roman","serif"'> <a name="OLE_LINK7"></a><a | |
| 122 name="OLE_LINK6"></a><a name="OLE_LINK3">Shows the number of mutations at GC | |
| 123 locations / the total number of mutations (the percentage of total mutations | |
| 124 that are at GC locations).</a> </span></p> | |
| 125 | |
| 126 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 127 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Transitions | |
| 128 at AT:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 129 font-family:"Times New Roman","serif"'> Shows the number of transitions at AT | |
| 130 locations (T>C, A>G) / the total number of mutations at AT locations (the | |
| 131 percentage of mutations at AT locations that are transitions).</span></p> | |
| 132 | |
| 133 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 134 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Targeting | |
| 135 of AT:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 136 font-family:"Times New Roman","serif"'> Shows the number of mutations at AT | |
| 137 locations / the total number of mutations (the percentage of total mutations | |
| 138 that are at AT locations).</span></p> | |
| 139 | |
| 140 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 141 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>RGYW:</span></i><span | |
| 142 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 143 <a name="OLE_LINK28"></a><a name="OLE_LINK27"></a><a name="OLE_LINK26">Shows | |
| 144 the number of mutations that are in a RGYW motive / The number of total mutations | |
| 145 (the percentage of mutations that are in a RGYW motive). </a><a | |
| 146 name="OLE_LINK62"></a><a name="OLE_LINK61">RGYW motives are known to be | |
| 147 preferentially targeted by AID </a></span><span lang=EN-GB style='font-size: | |
| 148 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>(R=Purine, | |
| 149 Y=pyrimidine, W = A or T).</span></p> | |
| 150 | |
| 151 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 152 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>WRCY:</span></i><span | |
| 153 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 154 <a name="OLE_LINK34"></a><a name="OLE_LINK33">Shows the number of mutations | |
| 155 that are in a </a><a name="OLE_LINK32"></a><a name="OLE_LINK31"></a><a | |
| 156 name="OLE_LINK30"></a><a name="OLE_LINK29">WRCY</a> motive / The number of | |
| 157 total mutations (the percentage of mutations that are in a WRCY motive). WRCY | |
| 158 motives are known to be preferentially targeted by AID </span><span lang=EN-GB | |
| 159 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>(R=Purine, | |
| 160 Y=pyrimidine, W = A or T).</span></p> | |
| 161 | |
| 162 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 163 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>WA:</span></i><span | |
| 164 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 165 <a name="OLE_LINK37"></a><a name="OLE_LINK36"></a><a name="OLE_LINK35">Shows | |
| 166 the number of mutations that are in a WA motive / The number of total mutations | |
| 167 (the percentage of mutations that are in a WA motive). It is described that | |
| 168 polymerase eta preferentially makes errors at WA motives </a></span><span | |
| 169 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>(W | |
| 170 = A or T).</span></p> | |
| 171 | |
| 172 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 173 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>TW:</span></i><span | |
| 174 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 175 Shows the number of mutations that are in a TW motive / The number of total mutations | |
| 176 (the percentage of mutations that are in a TW motive). It is described that | |
| 177 polymerase eta preferentially makes errors at TW motives </span><span | |
| 178 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>(W | |
| 179 = A or T).</span></p> | |
| 180 | |
| 181 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB | |
| 182 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Antigen | |
| 183 selection:</span></u></p> | |
| 184 | |
| 185 <p class=MsoNormalCxSpMiddle style='text-align:justify'><span lang=EN-GB | |
| 186 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>These | |
| 187 values give insight into antigen selection. It has been described that during | |
| 188 antigen selection, there is selection against replacement mutations in the FR | |
| 189 regions as these can cause instability of the B-cell receptor. In contrast | |
| 190 replacement mutations in the CDR regions are important for changing the | |
| 191 affinity of the B-cell receptor and therefore there is selection for this type | |
| 192 of mutations. Silent mutations do not alter the amino acid sequence and | |
| 193 therefore do not play a role in selection. More information on the values found | |
| 194 in healthy individuals of different ages can be found in IJspeert and van | |
| 195 Schouwenburg et al, PMID: 27799928</span></p> | |
| 196 | |
| 197 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 198 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>FR | |
| 199 R/S:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 200 font-family:"Times New Roman","serif"'> <a name="OLE_LINK43"></a><a | |
| 201 name="OLE_LINK42"></a><a name="OLE_LINK41">Shows the number of replacement | |
| 202 mutations in the FR regions / The number of silent mutations in the FR regions | |
| 203 (the number of replacement mutations in the FR regions divided by the number of | |
| 204 silent mutations in the FR regions)</a></span></p> | |
| 205 | |
| 206 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 207 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>CDR | |
| 208 R/S:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 209 font-family:"Times New Roman","serif"'> Shows the number of replacement | |
| 210 mutations in the CDR regions / The number of silent mutations in the CDR | |
| 211 regions (the number of replacement mutations in the CDR regions divided by the | |
| 212 number of silent mutations in the CDR regions)</span></p> | |
| 213 | |
| 214 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB | |
| 215 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Number | |
| 216 of sequences nucleotides:</span></u></p> | |
| 217 | |
| 218 <p class=MsoNormalCxSpMiddle style='text-align:justify'><span lang=EN-GB | |
| 219 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>These | |
| 220 values give information on the number of sequenced nucleotides.</span></p> | |
| 221 | |
| 222 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 223 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Nt | |
| 224 in FR:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 225 font-family:"Times New Roman","serif"'> <a name="OLE_LINK46"></a><a | |
| 226 name="OLE_LINK45"></a><a name="OLE_LINK44">Shows the number of sequences bases | |
| 227 that are located in the FR regions / The total number of sequenced bases (the | |
| 228 percentage of sequenced bases that are present in the FR regions).</a></span></p> | |
| 229 | |
| 230 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 231 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Nt | |
| 232 in CDR:</span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 233 font-family:"Times New Roman","serif"'> Shows the number of sequenced bases | |
| 234 that are located in the CDR regions / <a name="OLE_LINK48"></a><a | |
| 235 name="OLE_LINK47">The total number of sequenced bases (the percentage of | |
| 236 sequenced bases that are present in the CDR regions).</a></span></p> | |
| 237 | |
| 238 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 239 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>A: | |
| 240 </span></i><a name="OLE_LINK51"></a><a name="OLE_LINK50"></a><a | |
| 241 name="OLE_LINK49"><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 242 font-family:"Times New Roman","serif"'>Shows the total number of sequenced | |
| 243 adenines / The total number of sequenced bases (the percentage of sequenced | |
| 244 bases that were adenines).</span></a></p> | |
| 245 | |
| 246 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 247 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>C: | |
| 248 </span></i><a name="OLE_LINK53"></a><a name="OLE_LINK52"><span lang=EN-GB | |
| 249 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Shows | |
| 250 the total number of sequenced cytosines / The total number of sequenced bases | |
| 251 (the percentage of sequenced bases that were cytosines).</span></a></p> | |
| 252 | |
| 253 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 254 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>T: | |
| 255 </span></i><a name="OLE_LINK57"></a><a name="OLE_LINK56"><span lang=EN-GB | |
| 256 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Shows | |
| 257 the total number of sequenced </span></a><a name="OLE_LINK55"></a><a | |
| 258 name="OLE_LINK54"><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 259 font-family:"Times New Roman","serif"'>thymines</span></a><span lang=EN-GB | |
| 260 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'> | |
| 261 / The total number of sequenced bases (the percentage of sequenced bases that | |
| 262 were thymines).</span></p> | |
| 263 | |
| 264 <p class=MsoNormalCxSpMiddle style='text-align:justify'><i><span lang=EN-GB | |
| 265 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>G: | |
| 266 </span></i><span lang=EN-GB style='font-size:12.0pt;line-height:115%; | |
| 267 font-family:"Times New Roman","serif"'>Shows the total number of sequenced <a | |
| 268 name="OLE_LINK59"></a><a name="OLE_LINK58">guanine</a>s / The total number of | |
| 269 sequenced bases (the percentage of sequenced bases that were guanines).</span></p> | |
| 270 | |
| 271 <p class=MsoNormalCxSpMiddle style='text-align:justify'><a name="OLE_LINK69"><b><span | |
| 272 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Graphs</span></b></a></p> | |
| 273 | |
| 274 <p class=MsoNormalCxSpMiddle style='text-align:justify'><a name="OLE_LINK75"></a><a | |
| 275 name="OLE_LINK74"></a><a name="OLE_LINK73"><span lang=EN-GB style='font-size: | |
| 276 12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>These graphs visualize | |
| 277 information on the patterns and targeting of SHM and thereby give information | |
| 278 into the repair pathways used to repair the U:G mismatches introduced by AID. The | |
| 279 data represented in these graphs can be downloaded in the download tab. More | |
| 280 information on the values found in healthy individuals of different ages can be | |
| 281 found in IJspeert and van Schouwenburg et al, PMID: 27799928</span></a><span | |
| 282 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>. | |
| 283 <a name="OLE_LINK85"></a><a name="OLE_LINK84"></a></span></p> | |
| 284 | |
| 285 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=EN-GB | |
| 286 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Percentage | |
| 287 of mutations in AID and pol eta motives</span></u></p> | |
| 288 | |
| 289 <p class=MsoNormalCxSpMiddle style='text-align:justify'><span lang=EN-GB | |
| 290 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Visualizes | |
| 291 <a name="OLE_LINK80"></a><a name="OLE_LINK79"></a><a name="OLE_LINK78">for each | |
| 292 (sub)class </a>the percentage of mutations that are present in AID (RGYW or | |
| 293 WRCY) or polymerase eta motives (WA or TW) in the different subclasses </span><span | |
| 294 lang=EN-GB style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>(R=Purine, | |
| 295 Y=pyrimidine, W = A or T).</span></p> | |
| 296 | |
| 297 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=NL | |
| 298 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Relative | |
| 299 mutation patterns</span></u></p> | |
| 300 | |
| 301 <p class=MsoNormalCxSpMiddle style='text-align:justify'><span lang=EN-GB | |
| 302 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Visualizes | |
| 303 for each (sub)class the distribution of mutations between mutations at AT | |
| 304 locations and transitions or transversions at GC locations. </span></p> | |
| 305 | |
| 306 <p class=MsoNormalCxSpMiddle style='text-align:justify'><u><span lang=NL | |
| 307 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Absolute | |
| 308 mutation patterns</span></u></p> | |
| 309 | |
| 310 <p class=MsoNormalCxSpLast style='text-align:justify'><span lang=EN-GB | |
| 311 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Visualized | |
| 312 for each (sub)class the percentage of sequenced AT and GC bases that are | |
| 313 mutated. The mutations at GC bases are divided into transition and transversion | |
| 314 mutations<a name="OLE_LINK77"></a><a name="OLE_LINK76">. </a></span></p> | |
| 315 | |
| 316 <p class=MsoNormal><span lang=NL style='font-size:12.0pt;line-height:115%; | |
| 317 font-family:"Times New Roman","serif"'>Hanna IJspeert, Pauline A. van | |
| 318 Schouwenburg, David van Zessen, Ingrid Pico-Knijnenburg, Gertjan J. Driessen, | |
| 319 Andrew P. Stubbs, and Mirjam van der Burg (2016). </span><span | |
| 320 style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif"'>Evaluation | |
| 321 of the Antigen-Experienced B-Cell Receptor Repertoire in Healthy Children and | |
| 322 Adults. In <i>Frontiers in Immunolog, 7, pp. e410-410. </i>[<a | |
| 323 href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066086/"><span | |
| 324 style='color:windowtext'>doi:10.3389/fimmu.2016.00410</span></a>][<a | |
| 325 href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5066086/"><span | |
| 326 style='color:windowtext'>Link</span></a>]</span></p> | |
| 327 | |
| 328 </div> | |
| 329 | |
| 330 </body> | |
| 331 | |
| 332 </html> |