view kleborate.xml @ 32:fc47c4057ca5 draft

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<tool id="kleboratetool" name="Kleborate" version="0.1.0">
  <description> a tool to screen genome assemblies of Klebsiella pneumoniae</description>
  <requirements>
    <requirement type="package" version="2.1.0">kleborate</requirement>
    <requirement type="package" version="2.0.0">kaptive</requirement>
  </requirements>

  <!--  kleborateold -a `echo $input | tr ',' ' '` $r $kaptive_k $kaptive_o -o $output  
  /home/linuxipg/Kleborate/kleborate-runner.py -->
  <command detect_errors="aggressive"><![CDATA[
       ## Creates symlinks for each input file based on the Galaxy 'element_identifier'
        ## Used so that a human-readable name appears in the output table (instead of 'dataset_xyz.dat')
        #set $named_input_files = ''
        #for $input_file in $input_files
            ## Add single quotes around each input file identifier
            #set $_input_file = "'{}'".format($input_file.element_identifier)
            ln -s '${input_file}' ${_input_file} &&
            #set $named_input_files = $named_input_files + ' ' + $_input_file
        #end for

        kleborate $r $kaptive_k $kaptive_o -o $output --min_identity $ident --min_coverage $cov -a $named_input_files 
]]></command>
 
  <inputs>
  <param format="fasta" name="input_files" type="data" label="Genome fasta file : " multiple="true" display="checkboxes"/>
 
  <param name="r" type="select" label="Turn on resistance genes screening (default: no resistance gene screening):">
            <option value="">no</option>
            <option value="-r">yes</option>
          </param>

          <param name="kaptive_k" type="select" label="Turn on Kaptive screening of K loci (default: do not run Kaptive for K loci):">
            <option value="">no</option>
            <option value="--kaptive_k">yes</option>
          </param>

          <param name="kaptive_o" type="select" label="Turn on Kaptive screening of O loci (default: do not run Kaptive for O loci):">
            <option value="">no</option>
            <option value="--kaptive_o">yes</option>
          </param>

         <param name="ident" type="text" area="false" value="90.0" label="Minimum alignment percent identity for main results:"/> 
         <param name="cov" type="text" area="false" value="80.0" label="Minimum alignment percent coverage for main results:"/>
   
 </inputs>

 <outputs>  
    <data format="tabular" name="output" />
 </outputs>

 <tests>
    <test>
    <param name="input" value="KP011_S63.scfd.fasta " />
    <param name="input" value="KP012_S64.scfd.fasta " />
        <param name="r" value="-r"/>
<param name="kaptive_k" value="--kaptive_k"/>
<param name="kaptive_o" value=""/>
        <output name="output" file="Kleborate_results.txt" />
    </test>
</tests>

  <help>
For full documentation, please go to the Kleborate wiki (https://github.com/katholt/Kleborate/wiki)
  </help>
  <citations>
    <citation type="doi">10.1101/2020.12.14.422303</citation>
    <citation type="doi">10.1038/s41467-021-24448-3</citation>
    <citation type="doi">10.1099/mgen.0.000102</citation>
  </citations> 
</tool>