comparison PanExplorer.xml @ 14:5a5c9a6b047b draft

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13:152d7c43478b

<tool id="PanExplorer" name="PanExplorer" version="1.0">
  <description> Bacterial pan-genome analysis </description>
  <requirements>
  <!--
 <requirement type="package" version="2.2.26">blast-legacy</requirement>
 <requirement type="package" version="7.480">mafft</requirement>

    <command><![CDATA[

export PANEX_PATH=${__tool_directory__};

perl ${__tool_directory__}/Perl/generateConfig.pl '$private_genomes' '$input' config.yaml '$private_genomes_fasta';

cat config.yaml >$logfile;

#end if
]]></command>


  <inputs>
  <param name="input" type="text" multiple="true" label="List of genbank identifiers" help="Coma separated list (ex: CP000235.1,CP001079.1,CP001759.1,CP015994.2)"/>
  <param name="private_genomes" type="data" format="zip" label="Zip of genbank or GFF files" optional="true"/>
  <param name="private_genomes_fasta" type="data" format="zip" label="Zip of Fasta files" optional="true"/>
  <param name="min_identity" type="text" value="80" label="Minimum percentage identity for BlastP" />
  <param type="select" name="software" label="Choose the pan-genome software">
    <option value="pgap">PGAP</option>
    <option value="roary">Roary</option>
    <option value="panacota">PanACoTA</option>
    <option value="orthofinder">OrthoFinder</option>
    <option value="cactus">Minigraph-Cactus</option>
    <option value="pggb">PanGenome Graph Builder (PGGB)</option>
  </param>
 </inputs>

 <outputs>
 <data format="txt" name="output" label="Pangenome presence absence matrix"/>
 <data format="newick" name="njtree" label="PanBased NJ tree"/>

 <data format="txt" name="distance_matrix" label="Accessory based distance matrix"/>
 <data format="vcf" name="vcf" label="VCF file"/>
 <data format="txt" name="logfile" label="Logfile"/>
 <data format="txt" name="roary_log" label="Roary Logfile"/>
</outputs>

</tool>

14:5a5c9a6b047b

<tool id="PanExplorer2" name="PanExplorer2" version="2.0">
  <description> Bacterial pan-genome analysis </description>
  <requirements>
  <!--
 <requirement type="package" version="2.2.26">blast-legacy</requirement>
 <requirement type="package" version="7.480">mafft</requirement>

    <command><![CDATA[

export PANEX_PATH=${__tool_directory__};


#if str($mode.mode) == "accessions":
	    perl ${__tool_directory__}/Perl/generateConfig.pl 'None' '$input' config.yaml 'None';
#else if str($mode.mode) == "genbanks":
	    perl ${__tool_directory__}/Perl/generateConfig.pl '$private_genomes' 'None' config.yaml 'None';
#else if str($mode.mode) == "fasta":
            perl ${__tool_directory__}/Perl/generateConfig.pl '$private_genomes' 'None' config.yaml '$private_genomes_fasta';
#end if

cat config.yaml >$logfile;

#end if
]]></command>


  <inputs>
	  <conditional name="mode">
                <param name = "mode" type="select" label="What is your inputs?">
                        <option value="accessions">Prokaryote genomes: List of Genbank assembly accessions (GCA)</option>
                        <option value="genbanks">Prokaryote genomes: Genbank files</option>
                        <option value="fasta">Eukaryote genomes: FASTA + GFF files</option>
                </param>
                <when value="accessions">
			<param name="input" type="text" multiple="true" label="List of genbank identifiers" help="Coma separated list (ex: GCA_000007385.1,GCA_000010025.1,GCA_000019585.2)"/>
			<param type="select" name="software" label="Choose the pan-genome software">
				<option value="roary">Roary</option>
				<option value="panacota">PanACoTA</option>
				<option value="pggb">PanGenome Graph Builder (PGGB)</option>
			</param>
                </when>
                <when value="genbanks">
			<param name="private_genomes" type="data" format="zip" label="Zip of genbank files" optional="true"/>
			<param type="select" name="software" label="Choose the pan-genome software">
                                <option value="roary">Roary</option>
                                <option value="panacota">PanACoTA</option>
                                <option value="pggb">PanGenome Graph Builder (PGGB)</option>
                        </param>
		</when>
		<when value="fasta">
			<param name="private_genomes_fasta" type="data" format="zip" label="Zip of Fasta files" optional="true"/>
			<param name="private_genomes" type="data" format="zip" label="Zip of GFF files" optional="true"/>
			<param type="select" name="software" label="Choose the pan-genome software">
				<option value="orthofinder">OrthoFinder</option>
				<option value="cactus">Minigraph-Cactus</option>
				<option value="pggb">PanGenome Graph Builder (PGGB)</option>
			</param>
                </when>
	</conditional>

  <param name="min_identity" type="text" value="80" label="Minimum percentage identity for BlastP" />
 </inputs>

 <outputs>
 <data format="txt" name="output" label="Pangenome presence absence matrix"/>
 <data format="newick" name="njtree" label="PanBased NJ tree"/>

 <data format="txt" name="distance_matrix" label="Accessory based distance matrix"/>
 <data format="vcf" name="vcf" label="VCF file"/>
 <data format="txt" name="logfile" label="Logfile"/>
 <data format="txt" name="roary_log" label="Roary Logfile"/>
</outputs>
<tests>
        <test>
            <param name="input" value="GCA_000007385.1,GCA_000010025.1,GCA_000019585.2"/>
            <param name="min_identity" value="80"/>
            <param name="software" value="panacota"/>
            <param name="private_genomes" value=""/>
            <param name="private_genomes_fasta" value=""/>
            <output name="distance_matrix" value="Accessory_based_distance_matrix.txt"/>
            <output name="fastani" value="ANI.txt"/>
        </test>
 </tests>
 <help>

PanExplorer
=======

	 PanExplorer workflow is a snakemake worklow that can be run in the backend of the PanExplorer web application.

	 Homepage: https://panexplorer.southgreen.fr/

	 It allows to perform a pan-genome analysis using published and annotated bacteria genomes, using different tools that can be invoked: Roary, PGAP, PanACoTA.

	 Pangenome graph builder softwares have been implemented recently in the pipeline: Minigraph-Cactus and PGGB (PanGenome Graph Builder)

	 It provides a presence/absence matrix of genes, an UpsetR Diagram for synthetizing the matrix information and a COG assignation summary for each strain.
	 
Please visit the GitHub page for the PanExplorer workflow at: https://github.com/SouthGreenPlatform/PanExplorer_workflow


Inputs
------

	 Inputs can be provided as one of the following:

	 * **List of genbank assembly identifiers** comma-separated(ex: GCA_000007385.1,GCA_000010025.1,GCA_000019585.2)
	 * **Zip of genbank files** They must include the gene annotation and the complete sequence data
	 * **Zip of FASTA file of genomes + Zip of GFF annotation files**: In order to make the association between sequence and annotation, they must be named with the same basename as follows: genome1.fasta, genome1.gff, myspeciesXXX.fasta, myspeciesXXX.gff...

	

Outputs
------

	 Among the outputs:

	 * **Pangenome presence absence matrix** Pangene presence/absence matrix indicating the PAV (Presence Absence Variation) of clustered genes.
	 * **PanBased NJ tree** Distance tree based on PAV data
	 * **Heaps law alpha** Estimating if a pan-genome is open or closed based on a Heaps law model.
	 * **Rarefaction curves** A rarefaction curve is the cumulative number of gene clusters we observe as more and more genomes are being considered
	 * **ANI** Average Nucleotide Identity between genomes
	 * **ANI heatmap** image as SVG
	 * **VCF file** If a pan-genome graph software has been selected, it provides a VCF of variations among all samples.



    </help>
    <citations>
        <citation type="doi">10.1093/bioinformatics/btac504</citation>
    </citations>

</tool>