diff PanExplorer_workflow/Perl/ProjectPAVinCircos.pl @ 1:032f6b3806a3 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/PanExplorer_workflow/Perl/ProjectPAVinCircos.pl	Thu May 30 11:16:08 2024 +0000
@@ -0,0 +1,154 @@
+#!/usr/bin/perl
+
+use strict;
+
+my $pav = $ARGV[0];
+my $ref = $ARGV[1];
+my $strain_info = $ARGV[2];
+my $gff = $ARGV[3];
+my $ordering = $ARGV[4];
+my $outfile = $ARGV[5];
+
+my $circos_exe = "circos/bin/circos";
+#my $circos_exe = "circos";
+
+my %metadata;
+open(M,$strain_info);
+while(my $line = <M>){
+	chomp($line);
+	my ($id,$name) = split(/\t/,$line);
+	$metadata{$id} = $name;
+	$metadata{$name} = $id;
+}
+close(M);
+
+
+my $pangenome_size = 0;
+my $nb_core_genes = 0;
+my $nb_dispensable_genes = 0;
+my $nb_specific_genes = 0;
+my %cluster_of_gene;
+my %genes_of_cluster;
+my %tagging;
+open(P,$pav);
+my $firstline = <P>;
+chomp($firstline);
+my @infos = split(/\t/,$firstline);
+my %samples;
+my %hash_presence;
+my $col;
+for (my $j = 1; $j <= $#infos; $j++){
+	my $val = $infos[$j];
+	if ($val eq $ref){$col = $j;}
+	$samples{$j} = $val;
+}
+while(my $line = <P>){
+	chomp($line);
+	my @infos = split(/\t/,$line);
+	my $cluster_num = $infos[0];
+        my $clnb = $infos[0];
+        my $pos = $clnb;
+        my $pos_before = $pos - 1;
+        $pangenome_size++;
+        if (length($clnb) == 1){$clnb = "000".$clnb;}
+        elsif (length($clnb) == 2){$clnb = "00".$clnb;}
+        elsif (length($clnb) == 3){$clnb = "0".$clnb;}
+        my $name = "CLUSTER".$clnb;
+
+	# exclude lines where several genes for one strains
+	if ($line =~/,/){next;}
+
+	my $gene_ref = $infos[$col];
+	for (my $j = 1; $j <= $#infos; $j++){
+		my $gene = $infos[$j];
+		my $sample = $samples{$j};
+		if ($gene =~/\w+/){
+			$hash_presence{$gene_ref}{$sample} = 1;
+		}
+	}
+}
+close(P);
+
+my $track_thin = 0.5 / scalar keys(%samples);
+my $r0 = 0.4;
+my $r1 = $r0+$track_thin;
+my $highlight_block = "";
+my %max_position;
+open(O,$ordering);
+<O>;
+while(my $sample_name = <O>){
+#foreach my $sample(keys(%samples)){
+	chomp($sample_name);
+	#my $sample_name = $samples{$sample};
+	my $sample_id = $metadata{$sample_name};
+	print "$sample_name $sample_id\n";
+	my $ref_id = $metadata{$ref};
+	open(GFF,$gff);
+	open(CIRCOS_TRACK,">$outfile.$sample_id.circos.heatmap.txt");
+	my $current_start;
+	my $current_stop;
+	my $current_chrom;
+	$r0+=$track_thin;
+	$r1+=$track_thin;
+	#my $r1_final = $r1-($track_thin/3);
+	my $r1_final = $r1;
+	$r0.="r";
+	$r1_final.="r";	
+	$highlight_block .= qq~
+<highlight>
+file       = $outfile.$sample_id.circos.heatmap.txt
+r0         = $r0
+r1         = $r1_final
+</highlight>
+~;
+	while(my $line = <GFF>){
+		chomp($line);
+		my @infos = split(/\t/,$line);
+		if ($infos[2] eq "gene" && $line =~/ID=([^;]+);*/){
+			my $chr = $infos[0];
+			my $start = $infos[3];
+			my $end = $infos[4];
+			$current_start = $start;
+			$current_stop = $end;
+			$current_chrom = $chr;
+			if ($end > $max_position{$current_chrom}){$max_position{$current_chrom} = $end;}
+			my $gene = $1;
+			my $genelength = $end-$start;
+			my $gene_complete = $sample_name.":".$gene;
+			if ($hash_presence{$gene}{$sample_name}){
+				print CIRCOS_TRACK "$current_chrom $current_start $current_stop fill_color=red\n";
+			}	
+		}
+		if ($infos[2] eq "CDS" && $line =~/Parent=([^;]+);*/){
+			my $gene = $1;
+			my $gene_complete = $sample_name.":".$gene;
+			if ($line =~/protein_id=([^;]+);/){
+				$gene = $1;
+				if ($hash_presence{$gene}{$sample_name}){
+					print CIRCOS_TRACK "$current_chrom $current_start $current_stop fill_color=red\n";
+				}
+			}
+		}	
+	}
+	close(GFF);
+	close(CIRCOS_TRACK);
+}
+open(KARYOTYPE,">$outfile.karyotype.txt");
+foreach my $chr(keys(%max_position)){
+	my $max = $max_position{$chr};
+	print KARYOTYPE "chr - $chr 1 0 $max black";
+}
+close(KARYOTYPE);
+
+open(F,"circos_templates/circos1.conf");
+open(CIRCOS_CONF,">$outfile.circos1.conf");
+while(<F>){
+	if (/HIGHLIGHT_BLOCK/){print CIRCOS_CONF $highlight_block;}
+	elsif (/KARYOTYPE_PATH/){print CIRCOS_CONF "karyotype = $outfile.karyotype.txt\n";}
+	elsif (/OUTPUT_PNG/){print CIRCOS_CONF "file  = $outfile\n";}
+	else{print CIRCOS_CONF $_;}
+}
+close(F);
+close(CIRCOS_CONF);
+
+system("$circos_exe -conf $outfile.circos1.conf");