Mercurial > repos > dereeper > pangenome_explorer
view Snakemake_files/Snakefile_pggb_heatmap_upset @ 6:35d71348e81e draft
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author | dereeper |
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date | Thu, 30 May 2024 12:12:30 +0000 |
parents | e42d30da7a74 |
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import glob import os import shutil import yaml configfile: "config.yaml" SAMPLES = [] with open("config.yaml", "r") as yaml_file: genome_data = yaml.safe_load(yaml_file) for genome_name, genome_info in genome_data["input_genomes"].items(): name = genome_info["name"] SAMPLES.append(name) rule all: input: "outputs/seqfile", "outputs/pggb_out/all_genomes.fa.smooth.final.gfa", "outputs/pav_matrix.tsv", "outputs/heatmap.svg.gz", "outputs/rarefaction_curves.txt" rule get_data: input: "config.yaml" output: "outputs/seqfile", expand("outputs/genomes/{sample}.fasta", sample=SAMPLES), expand("outputs/genomes/{sample}.gff", sample=SAMPLES), run: with open(input[0], "r") as yaml_file, open(output[0], "w") as output_file: genome_data = yaml.safe_load(yaml_file) for genome_name, genome_info in genome_data["input_genomes"].items(): fasta_file = genome_info["fasta"] name = genome_info["name"] output_file.write(f"{genome_name}\t{genome_name}\t{fasta_file}\n") cmd = "sed 's/>/>"+str(name)+"#/g' "+fasta_file+" >outputs/genomes/"+str(name)+".fasta" os.system(cmd) gff_file = genome_info["gff3"] shutil.copy(gff_file, "outputs/genomes/"+str(name)+".gff") rule pggb: input: expand("outputs/genomes/{sample}.fasta", sample=SAMPLES), expand("outputs/genomes/{sample}.gff", sample=SAMPLES), output: gfa="outputs/pggb_out/all_genomes.fa.smooth.final.gfa", png1="outputs/pggb_out/all_genomes.fa.lay.draw.png", png2="outputs/pggb_out/all_genomes.fa.og.viz_multiqc.png", shell: """ cat outputs/genomes/*fasta >outputs/genomes/all_genomes.fa samtools faidx outputs/genomes/all_genomes.fa reference=$(head -1 outputs/genomes/strains.txt | awk '{{print $2}}') pggb -i outputs/genomes/all_genomes.fa -o outputs/pggb_out -V $reference -m mv outputs/pggb_out/all_genomes.*smooth.final.gfa {output.gfa} mv outputs/pggb_out/all_genomes.*lay.draw.png {output.png1} mv outputs/pggb_out/all_genomes.fa.*.og.viz_multiqc.png {output.png2} """ rule create_gene_paths: input: gff="outputs/genomes/{sample}.gff", gfa="outputs/pggb_out/all_genomes.fa.smooth.final.gfa", output: basename="outputs/genomes/{sample}.gene_segments", gene_length="outputs/genomes/{sample}.gene_segments.gene_length.txt", bed="outputs/genomes/{sample}.gene_segments.bed", shell: """ perl $PANEX_PATH/Perl/CreateGenePathsFromGFA.pl {input.gfa} {input.gff} {output.basename} """ rule bedtools_intersect: input: samples="outputs/seqfile", bedfiles=expand("outputs/genomes/{sample}.gene_segments.bed", sample=SAMPLES) params: identity=30 output: "outputs/pav_matrix.tsv", shell: """ perl $PANEX_PATH/Perl/GeneratePAVfromBed.pl {input.samples} outputs/genomes {output} {params.identity} """ rule heatmap_upset: input: pav="outputs/pav_matrix.tsv" output: heatmap="outputs/heatmap.svg.gz", html="outputs/heatmap.svg.heatmap_plotly.html", upsetr="outputs/upsetr.svg", binpav="outputs/heatmap.svg.pangenes_01matrix.txt" shell: """ perl $PANEX_PATH/Perl/GenerateHeatmapFromPAV.pl {input.pav} outputs/heatmap.svg mv outputs/heatmap.svg.upsetr.svg {output.upsetr} """ rule micropan: input: binpav="outputs/heatmap.svg.pangenes_01matrix.txt" output: txt="outputs/rarefaction_curves.txt", pdf="outputs/rarefaction_curves.pdf", svg="outputs/rarefaction_curves.svg", heaps="outputs/heaps.tsv" shell: """ Rscript $PANEX_PATH/R/micropan_rarefaction.R -f {input.binpav} -p {output.pdf} -a {output.heaps} -o {output.txt} pdf2svg {output.pdf} {output.svg} """