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view Roary/t/Bio/Roary/CommandLine/Roary.t @ 0:c47a5f61bc9f draft
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author | dereeper |
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date | Fri, 14 May 2021 20:27:06 +0000 |
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#!/usr/bin/env perl use Moose; use Data::Dumper; use File::Path qw( remove_tree); use File::Which; use File::Path qw(make_path); use Cwd qw(abs_path getcwd); use File::Find::Rule; #Test changes current working directory so relative paths can get out of sync local $ENV{PERL5LIB} = join(':', ("$ENV{PERL5LIB}", abs_path('./lib'), abs_path('./t/lib'))); local $ENV{PATH} = join(':', ("$ENV{PATH}", abs_path('./bin'))); BEGIN { unshift( @INC, abs_path('./lib') ) } BEGIN { unshift( @INC, abs_path('./t/lib') ) } with 'TestHelper'; BEGIN { use Test::Most; use_ok('Bio::Roary::CommandLine::Roary'); use_ok('Bio::Roary::CommandLine::CreatePanGenome'); use Bio::Roary::SequenceLengths; } my $script_name = 'Bio::Roary::CommandLine::Roary'; my $cwd = getcwd(); local $ENV{PATH} = "$ENV{PATH}:./bin"; my %scripts_and_expected_files; system('touch empty_file'); cleanup_files(); %scripts_and_expected_files = ( ' -j Parallel --dont_split_groups t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff ' => [ 'gene_presence_absence.csv', 't/data/overall_gene_presence_absence.csv' ], ' -j Local -t 1 --dont_split_groups t/data/genbank_gbff/genbank1.gff t/data/genbank_gbff/genbank2.gff t/data/genbank_gbff/genbank3.gff' => [ 'gene_presence_absence.csv', 't/data/genbank_gbff/genbank_gene_presence_absence.csv' ], '-h' => [ 'empty_file', 't/data/empty_file' ], ); mock_execute_script_and_check_output_sorted( $script_name, \%scripts_and_expected_files, [ 0, 6, 7, 8, 9 ] ); cleanup_files(); stderr_should_have($script_name,'-a', 'Looking for'); my $current_cwd = getcwd(); stderr_should_have($script_name,'-v --output_directory t/data/directory_which_doesnt_exist t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff', 'Output directory created'); ok( ( -e 't/data/directory_which_doesnt_exist/clustered_proteins' ), 'pan genome files should be in directory' ); is(getcwd(),$current_cwd , 'current working directory should not have changed after script is finished'); SKIP: { skip "prank not installed", 11 unless ( which('prank') ); %scripts_and_expected_files = ( '-j Local --dont_delete_files --dont_split_groups --output_multifasta_files t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'pan_genome_sequences/mdoH.fa.aln', 't/data/mdoH.fa.aln' ], ); mock_execute_script_and_check_output( $script_name, \%scripts_and_expected_files ); ok( -e 'core_gene_alignment.aln', 'Core gene alignment exists' ); ok( my $seq_len = Bio::Roary::SequenceLengths->new( fasta_file => 'core_gene_alignment.aln', ), 'Check size of the core_gene_alignment.aln init' ); my @keys = keys %{ $seq_len->sequence_lengths }; is( $seq_len->sequence_lengths->{ $keys[0] }, 64983, 'length of first sequence' ); ok( -e 'core_alignment_header.embl', 'Core gene alignment header exists' ); ok( -e 'accessory.tab' ); ok( -e 'core_accessory.tab' ); ok( -e 'number_of_conserved_genes.Rtab' ); ok( -e 'number_of_genes_in_pan_genome.Rtab' ); ok( -e 'number_of_new_genes.Rtab' ); ok( -e 'number_of_unique_genes.Rtab' ); ok( -e 'blast_identity_frequency.Rtab' ); cleanup_files(); %scripts_and_expected_files = ( '-j Local --output_multifasta_files t/data/core_alignment_gene_lookup/query_1.gff t/data/core_alignment_gene_lookup/query_2.gff t/data/core_alignment_gene_lookup/query_3.gff' => [ 'core_gene_alignment.aln', 't/data/core_alignment_gene_lookup/expected_core_gene_alignment.aln' ], ); mock_execute_script_and_check_output( $script_name, \%scripts_and_expected_files ); cleanup_files(); } SKIP: { skip "extended tests not run", 40 unless ( defined($ENV{ROARY_FULL_TESTS})); %scripts_and_expected_files = ( '-o some_different_output -i 90 -p 2 --translation_table 1 t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'some_different_output', 't/data/expected_some_different_output' ], ); mock_execute_script_and_check_output_sorted( $script_name, \%scripts_and_expected_files, [ 0 ] ); stderr_should_have($script_name,'--translation_table 1 -o some_different_output --core_definition 60 -p 2 -e --mafft --group_limit 10 t/data/real_data_1.gff t/data/real_data_2.gff', 'Exiting early because number of clusters is too high'); stderr_should_have($script_name,'--verbose_stats --group_limit 10 -e t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff', 'Exiting early because number of clusters is too high'); stderr_should_not_have($script_name,'-e --group_limit 10 t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff ', 'Cant access the multifasta base directory'); stderr_should_have($script_name,'-i 90 --core_definition 60 -p 2 -v t/data/real_data_1.gff t/data/real_data_2.gff ','Cleaning up files'); stderr_should_have($script_name,'-i 30 t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff','The percentage identity is too low'); stderr_should_not_have($script_name,'--dont_delete_files -v t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff ','Cleaning up files'); stderr_should_have($script_name,'-v --group_limit 100000 -e t/data/query_1.gff t/data/query_2.gff t/data/query_5.gff ' ,'Running command: pan_genome_core_alignment'); stderr_should_have($script_name,'--translation_table 1 -v t/data/real_data_1.gff t/data/real_data_2.gff ' ,'Cleaning up files'); stderr_should_have($script_name,'-e -v t/data/real_data_1.gff t/data/real_data_2.gff ','Creating files with the nucleotide sequences for every cluster'); SKIP: { skip "kraken not installed", 2 unless ( which('kraken') ); skip "kraken-report not installed", 2 unless ( which('kraken-report') ); stderr_should_have($script_name,'-v --qc t/data/real_data_1.gff t/data/real_data_2.gff' ,'Running Kraken on each input assembly'); } %scripts_and_expected_files = ( # output '-o some_different_output -e --dont_delete_files t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'pan_genome_sequences/mdoH.fa.aln', 't/data/mdoH.fa.aln' ], '-o some_different_output --core_definition 60 t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'summary_statistics.txt', 't/data/expected_core_60_summary_statistics.txt' ], '-e -i 95.3 --translation_table 1 -v --group_limit 100000 --qc t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'core_gene_alignment.aln', 't/data/expected_real_data_core_gene_alignment.aln' ], '-e --verbose_stats t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'core_gene_alignment.aln', 't/data/expected_real_data_core_gene_alignment.aln' ], '--core_definition 60 t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'summary_statistics.txt', 't/data/expected_core_60_summary_statistics.txt' ], '-p 2 -e --dont_delete_files t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'pan_genome_sequences/mdoH.fa.aln', 't/data/mdoH.fa.aln' ], '-p 2 --core_definition 60 t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'summary_statistics.txt', 't/data/expected_core_60_summary_statistics.txt' ], '-p 2 -e --mafft t/data/real_data_1.gff t/data/real_data_2.gff' => [ 'core_gene_alignment.aln', 't/data/expected_mafft_real_data_core_gene_alignment.aln' ], ); mock_execute_script_and_check_output_sorted( $script_name, \%scripts_and_expected_files ); } cleanup_files(); done_testing(); sub cleanup_files { remove_tree('pan_genome_sequences'); remove_tree('fixed_input_files'); remove_tree('t/data/directory_which_doesnt_exist'); remove_tree('locus_tags_gffs_output'); unlink('_blast_results'); unlink('_clustered'); unlink('_clustered.bak.clstr'); unlink('_clustered.clstr'); unlink('_combined_files'); unlink('_combined_files.groups'); unlink('_fasta_files'); unlink('_gff_files'); unlink('_inflated_mcl_groups'); unlink('_inflated_unsplit_mcl_groups'); unlink('_labeled_mcl_groups'); unlink('_uninflated_mcl_groups'); unlink('accessory.header.embl'); unlink('accessory.header.tab'); unlink('accessory.tab'); unlink('blast_identity_frequency.Rtab'); unlink('clustered_proteins'); unlink('core_accessory.header.embl'); unlink('core_accessory.header.tab'); unlink('core_accessory.tab'); unlink('core_gene_alignment.aln'); unlink('database_masking.asnb'); unlink('example_1.faa.tmp.filtered.fa'); unlink('example_2.faa.tmp.filtered.fa'); unlink('example_3.faa.tmp.filtered.fa'); unlink('gene_presence_absence.csv'); unlink('number_of_conserved_genes.Rtab'); unlink('number_of_genes_in_pan_genome.Rtab'); unlink('number_of_new_genes.Rtab'); unlink('number_of_unique_genes.Rtab'); unlink('pan_genome.fa'); unlink('query_1.gff.proteome.faa'); unlink('query_2.gff.proteome.faa'); unlink('query_3.gff.proteome.faa'); unlink('query_5.gff.proteome.faa'); unlink('real_data_1.gff.proteome.faa'); unlink('real_data_2.gff.proteome.faa'); unlink('pan_genome_reference.fa'); unlink('accessory_graph.dot'); unlink('core_accessory_graph.dot'); unlink('some_different_output'); unlink('core_alignment_header.embl'); }