# HG changeset patch
# User devteam
# Date 1487524325 18000
# Node ID e66b9b5b8580df969f96c418daff7c68134501c5
# Parent 1322b73ffe4485cf16e859dd61e22f2b136401a9
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/cufflinks/cuffcompare commit 82ee6fc860c52c531b7a57bbb346ab1a67a434a5
diff -r 1322b73ffe44 -r e66b9b5b8580 cuff_macros.xml
--- a/cuff_macros.xml Tue Feb 07 18:38:41 2017 -0500
+++ b/cuff_macros.xml Sun Feb 19 12:12:05 2017 -0500
@@ -8,14 +8,6 @@
-
-
-
-
-
-
-
-
diff -r 1322b73ffe44 -r e66b9b5b8580 cuffcompare_wrapper.py
--- a/cuffcompare_wrapper.py Tue Feb 07 18:38:41 2017 -0500
+++ b/cuffcompare_wrapper.py Sun Feb 19 12:12:05 2017 -0500
@@ -38,23 +38,6 @@
(options, args) = parser.parse_args()
- # output version # of tool
- try:
- with tempfile.NamedTemporaryFile() as tmp_stdout:
- returncode = subprocess.call(args='cuffcompare 2>&1', stdout=tmp_stdout, shell=True)
- stdout = None
- with open(tmp_stdout.name) as tmp_stdout2:
- for line in tmp_stdout2:
- if line.lower().find('cuffcompare v') >= 0:
- stdout = line.strip()
- break
- if stdout:
- sys.stdout.write('%s\n' % stdout)
- else:
- raise Exception
- except:
- sys.stdout.write('Could not determine Cuffcompare version\n')
-
# Set/link to sequence file.
if options.use_seq_data:
if options.ref_file:
diff -r 1322b73ffe44 -r e66b9b5b8580 cuffcompare_wrapper.xml
--- a/cuffcompare_wrapper.xml Tue Feb 07 18:38:41 2017 -0500
+++ b/cuffcompare_wrapper.xml Sun Feb 19 12:12:05 2017 -0500
@@ -1,12 +1,11 @@
-
+compare assembled transcripts to a reference annotation and track Cufflinks transcripts across multiple experimentscuff_macros.xml
- cuffcompare 2>&1 | head -n 1
-
+
+ ]]>
@@ -60,7 +59,7 @@
-
@@ -91,29 +90,29 @@
-
-
-
-
-
annotation['use_ref_annotation'] == 'Yes'@HAS_MULTIPLE_INPUTS@
-
annotation['use_ref_annotation'] == 'Yes' and @HAS_MULTIPLE_INPUTS@
@@ -124,7 +123,7 @@
-
@@ -173,7 +172,7 @@
Cuffcompare takes Cufflinks' GTF output as input, and optionally can take a "reference" annotation (such as from Ensembl_)
-.. _Ensembl: http://www.ensembl.org
+.. _Ensembl: http://www.ensembl.org
------
@@ -187,7 +186,7 @@
Transcripts Combined File:
-Cuffcompare reports a GTF file containing the "union" of all transfrags in each sample. If a transfrag is present in both samples, it is thus reported once in the combined gtf.
+Cuffcompare reports a GTF file containing the "union" of all transfrags in each sample. If a transfrag is present in both samples, it is thus reported once in the combined gtf.
Transcripts Tracking File:
@@ -209,7 +208,7 @@
3 Reference gene id Tcea The gene_name attribute of the reference GTF record for this transcript, or '-' if no reference transcript overlaps this Cufflinks transcript
4 Reference transcript id uc007afj.1 The transcript_id attribute of the reference GTF record for this transcript, or '-' if no reference transcript overlaps this Cufflinks transcript
5 Class code c The type of match between the Cufflinks transcripts in column 6 and the reference transcript. See class codes
-
+
Each of the columns after the fifth have the following format:
qJ:gene_id|transcript_id|FMI|FPKM|conf_lo|conf_hi
@@ -222,18 +221,18 @@
Priority Code Description
---------------------------------
1 = Match
- 2 c Contained
- 3 j New isoform
- 4 e A single exon transcript overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment.
+ 2 c Contained
+ 3 j New isoform
+ 4 e A single exon transcript overlapping a reference exon and at least 10 bp of a reference intron, indicating a possible pre-mRNA fragment.
5 i A single exon transcript falling entirely with a reference intron
6 o Generic exonic overlap with a reference transcript
- 7 p Possible polymerase run-on fragment
- 8 r Repeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the bases are lower case
+ 7 p Possible polymerase run-on fragment
+ 8 r Repeat. Currently determined by looking at the soft-masked reference sequence and applied to transcripts where at least 50% of the bases are lower case
9 u Unknown, intergenic transcript
10 x Exonic overlap with reference on the opposite strand
11 s An intron of the transfrag overlaps a reference intron on the opposite strand (likely due to read mapping errors)
12 . (.tracking file only, indicates multiple classifications)
-
+
-------
**Settings**
diff -r 1322b73ffe44 -r e66b9b5b8580 tool_dependencies.xml
--- a/tool_dependencies.xml Tue Feb 07 18:38:41 2017 -0500
+++ /dev/null Thu Jan 01 00:00:00 1970 +0000
@@ -1,6 +0,0 @@
-
-
-
-
-
-