comparison data_manager/bowtie2_index_builder.py @ 1:9bf4eb559ed5 draft

planemo upload commit 4381f4022eca6f3c58ae1d01096795cd28c15062
author devteam
date Mon, 10 Aug 2015 16:44:10 -0400
parents 9458af1ab90b
children 83da94c0e4a6
comparison
equal deleted inserted replaced
0:9458af1ab90b 1:9bf4eb559ed5
7 import subprocess 7 import subprocess
8 8
9 from json import loads, dumps 9 from json import loads, dumps
10 10
11 11
12 DEFAULT_DATA_TABLE_NAME = "bowtie2_indexes" 12 DEFAULT_DATA_TABLE_NAMES = ["bowtie2_indexes"]
13 13
14 def get_id_name( params, dbkey, fasta_description=None): 14 def get_id_name( params, dbkey, fasta_description=None):
15 #TODO: ensure sequence_id is unique and does not already appear in location file 15 #TODO: ensure sequence_id is unique and does not already appear in location file
16 sequence_id = params['param_dict']['sequence_id'] 16 sequence_id = params['param_dict']['sequence_id']
17 if not sequence_id: 17 if not sequence_id:
22 sequence_name = fasta_description 22 sequence_name = fasta_description
23 if not sequence_name: 23 if not sequence_name:
24 sequence_name = dbkey 24 sequence_name = dbkey
25 return sequence_id, sequence_name 25 return sequence_id, sequence_name
26 26
27 def build_bowtie2_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME ): 27 def build_bowtie2_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_names=DEFAULT_DATA_TABLE_NAMES ):
28 #TODO: allow multiple FASTA input files 28 #TODO: allow multiple FASTA input files
29 fasta_base_name = os.path.split( fasta_filename )[-1] 29 fasta_base_name = os.path.split( fasta_filename )[-1]
30 sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name ) 30 sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name )
31 os.symlink( fasta_filename, sym_linked_fasta_filename ) 31 os.symlink( fasta_filename, sym_linked_fasta_filename )
32 args = [ 'bowtie2-build', sym_linked_fasta_filename, sequence_id ] 32 args = [ 'bowtie2-build', sym_linked_fasta_filename, sequence_id ]
34 return_code = proc.wait() 34 return_code = proc.wait()
35 if return_code: 35 if return_code:
36 print >> sys.stderr, "Error building index." 36 print >> sys.stderr, "Error building index."
37 sys.exit( return_code ) 37 sys.exit( return_code )
38 data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=sequence_id ) 38 data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=sequence_id )
39 _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ) 39 for data_table_name in data_table_names:
40 _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry )
40 41
41 def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ): 42 def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ):
42 data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} ) 43 data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} )
43 data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] ) 44 data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] )
44 data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry ) 45 data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
48 #Parse Command Line 49 #Parse Command Line
49 parser = optparse.OptionParser() 50 parser = optparse.OptionParser()
50 parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' ) 51 parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' )
51 parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' ) 52 parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' )
52 parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' ) 53 parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' )
53 parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' ) 54 parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='append', type="string", default=None, help='data_table_name' )
54 (options, args) = parser.parse_args() 55 (options, args) = parser.parse_args()
55 56
56 filename = args[0] 57 filename = args[0]
57 58
58 params = loads( open( filename ).read() ) 59 params = loads( open( filename ).read() )
66 raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) ) 67 raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) )
67 68
68 sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description ) 69 sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description )
69 70
70 #build the index 71 #build the index
71 build_bowtie2_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME ) 72 build_bowtie2_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_names=options.data_table_name or DEFAULT_DATA_TABLE_NAMES )
72 73
73 #save info to json file 74 #save info to json file
74 open( filename, 'wb' ).write( dumps( data_manager_dict ) ) 75 open( filename, 'wb' ).write( dumps( data_manager_dict ) )
75 76
76 if __name__ == "__main__": main() 77 if __name__ == "__main__": main()