diff data_manager/data_manager_gatk_picard_index_builder.py @ 0:619dd9e9c09c draft

Imported from capsule None

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/data_manager/data_manager_gatk_picard_index_builder.py	Tue Apr 01 10:48:03 2014 -0400
@@ -0,0 +1,179 @@
+#!/usr/bin/env python
+# Dave B.
+# Uses fasta sorting functions written by Dan Blankenberg.
+
+import json
+import optparse
+import os
+import shutil
+import subprocess
+import sys
+import tempfile
+
+CHUNK_SIZE = 2**20
+
+DEFAULT_DATA_TABLE_NAME = "fasta_indexes"
+
+def get_id_name( params, dbkey, fasta_description=None):
+    #TODO: ensure sequence_id is unique and does not already appear in location file
+    sequence_id = params['param_dict']['sequence_id']
+    if not sequence_id:
+        sequence_id = dbkey
+    
+    sequence_name = params['param_dict']['sequence_name']
+    if not sequence_name:
+        sequence_name = fasta_description
+        if not sequence_name:
+            sequence_name = dbkey
+    return sequence_id, sequence_name
+
+def build_picard_index( data_manager_dict, fasta_filename, target_directory, dbkey, sequence_id, sequence_name, jar, data_table_name=DEFAULT_DATA_TABLE_NAME ):
+    fasta_base_name = os.path.split( fasta_filename )[-1]
+    gatk_sorted_fasta_filename = os.path.join( target_directory, fasta_base_name )
+    shutil.copy( fasta_filename, gatk_sorted_fasta_filename )
+    _sort_fasta_gatk( gatk_sorted_fasta_filename )
+    sam_index_filename = '%s.fai' % gatk_sorted_fasta_filename
+    if not os.path.exists( sam_index_filename ):
+        sam_command = [ 'samtools', 'faidx', gatk_sorted_fasta_filename ]
+        _run_command( sam_command, target_directory )
+    args = [ 'java', '-jar', jar, 'R=%s' % gatk_sorted_fasta_filename, 'O=%s.dict' % sequence_id ]
+    _run_command( args, target_directory )
+    data_table_entry = dict( value=sequence_id, dbkey=dbkey, name=sequence_name, path=fasta_base_name )
+    _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry )
+
+def _run_command( command, target_directory ):
+    tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-data-manager-gatk_picard_index_builder-stderr" )
+    proc = subprocess.Popen( args=command, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno() )
+    return_code = proc.wait()
+    if return_code:
+        tmp_stderr.flush()
+        tmp_stderr.seek( 0 )
+        sys.stderr.write( "Error building index:\n" )
+        while True:
+            chunk = tmp_stderr.read( CHUNK_SIZE )
+            if not chunk:
+                break
+            sys.stderr.write( chunk )
+        sys.exit( return_code )
+    tmp_stderr.close()
+
+def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ):
+    data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} )
+    data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] )
+    data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
+    return data_manager_dict
+
+def _move_and_index_fasta_for_sorting( fasta_filename ):
+    unsorted_filename = tempfile.NamedTemporaryFile().name
+    shutil.move( fasta_filename, unsorted_filename )
+    fasta_offsets = {}
+    unsorted_fh = open( unsorted_filename )
+    while True:
+        offset = unsorted_fh.tell()
+        line = unsorted_fh.readline()
+        if not line:
+            break
+        if line.startswith( ">" ):
+            line = line.split( None, 1 )[0][1:]
+            fasta_offsets[ line ] = offset
+    unsorted_fh.close()
+    current_order = map( lambda x: x[1], sorted( map( lambda x: ( x[1], x[0] ), fasta_offsets.items() ) ) )
+    return ( unsorted_filename, fasta_offsets, current_order )
+
+def _write_sorted_fasta( sorted_names, fasta_offsets, sorted_fasta_filename, unsorted_fasta_filename ):
+    unsorted_fh = open( unsorted_fasta_filename )
+    sorted_fh = open( sorted_fasta_filename, 'wb+' )
+    
+    for name in sorted_names:
+        offset = fasta_offsets[ name ]
+        unsorted_fh.seek( offset )
+        sorted_fh.write( unsorted_fh.readline() )
+        while True:
+            line = unsorted_fh.readline()
+            if not line or line.startswith( ">" ):
+                break
+            sorted_fh.write( line )
+    unsorted_fh.close()
+    sorted_fh.close()
+
+def _int_to_roman( integer ):
+    if not isinstance( integer, int ):
+        raise TypeError, "expected integer, got %s" % type( integer )
+    if not 0 < integer < 4000:
+        raise ValueError, "Argument must be between 1 and 3999, got %s" % str( integer )   
+    ints = ( 1000, 900, 500, 400, 100, 90, 50, 40, 10, 9, 5, 4, 1 )
+    nums = ( 'M', 'CM', 'D', 'CD', 'C', 'XC', 'L', 'XL', 'X', 'IX', 'V', 'IV', 'I' )
+    result = ""
+    for i in range( len( ints ) ):
+        count = int( integer / ints[ i ] )
+        result += nums[ i ] * count
+        integer -= ints[ i ] * count
+    return result
+
+def _sort_fasta_gatk( fasta_filename ):
+    ( unsorted_filename, fasta_offsets, current_order ) = _move_and_index_fasta_for_sorting( fasta_filename )
+    sorted_names = map( str, range( 1, 100 ) ) + map( _int_to_roman, range( 1, 100 ) ) + [ 'X', 'Y', 'M' ]
+    #detect if we have chrN, or just N
+    has_chr = False
+    for chrom in sorted_names:
+        if "chr%s" % chrom in current_order:
+            has_chr = True
+            break
+    
+    if has_chr:
+        sorted_names = map( lambda x: "chr%s" % x, sorted_names)
+    else:
+        sorted_names.insert( 0, "MT" )
+    sorted_names.extend( map( lambda x: "%s_random" % x, sorted_names ) )
+    
+    existing_sorted_names = []
+    for name in sorted_names:
+        # Append each chromosome only once.
+        if name in current_order and name not in existing_sorted_names:
+            existing_sorted_names.append( name )
+    for name in current_order:
+        #TODO: confirm that non-canonical names do not need to be sorted specially
+        if name not in existing_sorted_names:
+            existing_sorted_names.append( name )
+    
+    if existing_sorted_names == current_order:
+        shutil.move( unsorted_filename, fasta_filename )
+    else:
+        _write_sorted_fasta( existing_sorted_names, fasta_offsets, fasta_filename, unsorted_filename )
+
+def main():
+    #Parse Command Line
+    parser = optparse.OptionParser()
+    parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' )
+    parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' )
+    parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' )
+    parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' )
+    parser.add_option( '-j', '--jar', dest='jar', action='store', type="string", default=None, help='GATK .jar file' )
+    (options, args) = parser.parse_args()
+    
+    filename = args[0]
+    
+    params = json.loads( open( filename ).read() )
+    target_directory = params[ 'output_data' ][0]['extra_files_path']
+    os.mkdir( target_directory )
+    data_manager_dict = {}
+    
+    if options.fasta_dbkey in [ None, '', '?' ]:
+        raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( options.fasta_dbkey ) )
+    
+    sequence_id, sequence_name = get_id_name( params, dbkey=options.fasta_dbkey, fasta_description=options.fasta_description )
+    
+    #build the index
+    build_picard_index( data_manager_dict, 
+                        options.fasta_filename, 
+                        target_directory, 
+                        options.fasta_dbkey, 
+                        sequence_id, 
+                        sequence_name, 
+                        options.jar, 
+                        data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME )
+    
+    #save info to json file
+    open( filename, 'wb' ).write( json.dumps( data_manager_dict ) )
+        
+if __name__ == "__main__": main()