annotate data_manager/twobit_builder.py @ 3:5c77d4091880 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_twobit_builder commit 82ad45497a7b2277a710c634378a4122fb75ce1f"

#!/usr/bin/env python
# Dan Blankenberg
from __future__ import print_function

import optparse
import os
import subprocess
import sys
import tempfile
from json import dumps, loads

CHUNK_SIZE = 2**20  # 1mb


def get_id_name(params, dbkey, fasta_description=None):
    # TODO: ensure sequence_id is unique and does not already appear in location file
    sequence_id = params['param_dict']['sequence_id']
    if not sequence_id:
        sequence_id = dbkey  # uuid.uuid4() generate and use an uuid

    sequence_name = params['param_dict']['sequence_name']
    if not sequence_name:
        sequence_name = fasta_description
        if not sequence_name:
            sequence_name = dbkey
    return sequence_id, sequence_name


def build_twobit(data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name):
    twobit_base_name = "%s.2bit" % (sequence_id)
    twobit_filename = os.path.join(target_directory, twobit_base_name)

    args = ['faToTwoBit', fasta_filename, twobit_filename]
    tmp_stderr = tempfile.NamedTemporaryFile(prefix="tmp-data-manager-twobit-builder-stderr")
    proc = subprocess.Popen(args=args, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno())
    return_code = proc.wait()
    if return_code:
        tmp_stderr.flush()
        tmp_stderr.seek(0)
        print("Error building index:", file=sys.stderr)
        while True:
            chunk = tmp_stderr.read(CHUNK_SIZE)
            if not chunk:
                break
            sys.stderr.write(chunk)
        sys.exit(return_code)
    tmp_stderr.close()
    # lastz_seqs
    data_table_entry = dict(value=sequence_id, name=sequence_name, path=twobit_base_name)

    _add_data_table_entry(data_manager_dict, "lastz_seqs", data_table_entry)
    # twobit.loc
    data_table_entry = dict(value=sequence_id, path=twobit_base_name)

    _add_data_table_entry(data_manager_dict, "twobit", data_table_entry)
    # alignseq
    data_table_entry = dict(type="seq", value=sequence_id, path=twobit_base_name)

    _add_data_table_entry(data_manager_dict, "alignseq_seq", data_table_entry)


def _add_data_table_entry(data_manager_dict, data_table_name, data_table_entry):
    data_manager_dict['data_tables'] = data_manager_dict.get('data_tables', {})
    data_manager_dict['data_tables'][data_table_name] = data_manager_dict['data_tables'].get(data_table_name, [])
    data_manager_dict['data_tables'][data_table_name].append(data_table_entry)
    return data_manager_dict


def main():
    parser = optparse.OptionParser()
    parser.add_option('-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename')
    parser.add_option('-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey')
    parser.add_option('-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description')
    (options, args) = parser.parse_args()

    filename = args[0]

    params = loads(open(filename).read())

    target_directory = params['output_data'][0]['extra_files_path']
    os.mkdir(target_directory)
    data_manager_dict = {}

    dbkey = options.fasta_dbkey

    if dbkey in [None, '', '?']:
        raise Exception('"%s" is not a valid dbkey. You must specify a valid dbkey.' % (dbkey))

    sequence_id, sequence_name = get_id_name(params, dbkey=dbkey, fasta_description=options.fasta_description)

    # build the index
    build_twobit(data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name)

    # save info to json file
    open(filename, 'w').write(dumps(data_manager_dict, sort_keys=True))


if __name__ == "__main__":
    main()