# HG changeset patch # User devteam # Date 1447263397 18000 # Node ID f666895cbebdd876a8643df696f669d64c55b199 # Parent f2ab5b44870d47fff5baac9c52f32c0b0fc9fb6e planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/fastx_toolkit/fasta_clipping_histogram commit a1517c9d22029095120643bbe2c8fa53754dd2b7 diff -r f2ab5b44870d -r f666895cbebd fasta_clipping_histogram.xml --- a/fasta_clipping_histogram.xml Wed Sep 25 14:55:11 2013 -0400 +++ b/fasta_clipping_histogram.xml Wed Nov 11 12:36:37 2015 -0500 @@ -1,19 +1,20 @@ - chart + chart fastx_toolkit - fasta_clipping_histogram.pl $input $outfile - - - - + fasta_clipping_histogram.pl $input $outfile + + + + - - - - - + + + + + + **What it does** This tool creates a histogram image of sequence lengths distribution in a given fasta dataset file. @@ -24,21 +25,18 @@ **Output Examples** -In the following library, most sequences are 24-mers to 27-mers. +In the following library, most sequences are 24-mers to 27-mers. This could indicate an abundance of endo-siRNAs (depending of course of what you've tried to sequence in the first place). .. image:: ${static_path}/fastx_icons/fasta_clipping_histogram_1.png - -In the following library, most sequences are 19,22 or 23-mers. +In the following library, most sequences are 19,22 or 23-mers. This could indicate an abundance of miRNAs (depending of course of what you've tried to sequence in the first place). .. image:: ${static_path}/fastx_icons/fasta_clipping_histogram_2.png - ----- - **Input Formats** This tool accepts short-reads FASTA files. The reads don't have to be short, but they do have to be on a single line, like so:: @@ -50,7 +48,6 @@ >sequence3 CCTTGAGATTAACGCTAATCAAGTAAAC - If the sequences span over multiple lines:: >sequence1 @@ -63,11 +60,8 @@ >sequence1 CAGCATCTACATAATATGATCGCTATTAAACTTAAATCTCCTTGACGGAGTCTTCGGTCATAACACAAACCCAGACCTACGTATATGACAAAGCTAATAGaactggtctttacctTTAAGTTG - ----- - - **Multiplicity counts (a.k.a reads-count)** If the sequence identifier (the text after the '>') contains a dash and a number, it is treated as a multiplicity count value (i.e. how many times that individual sequence repeated in the original FASTA file, before collapsing). @@ -85,7 +79,6 @@ .. image:: ${static_path}/fastx_icons/fasta_clipping_histogram_3.png - Example 2 - The following FASTA file have multiplicity counts:: >seq1-2 @@ -106,7 +99,5 @@ This tool is based on `FASTX-toolkit`__ by Assaf Gordon. .. __: http://hannonlab.cshl.edu/fastx_toolkit/ - - - - \ No newline at end of file + + diff -r f2ab5b44870d -r f666895cbebd tool_dependencies.xml --- a/tool_dependencies.xml Wed Sep 25 14:55:11 2013 -0400 +++ b/tool_dependencies.xml Wed Nov 11 12:36:37 2015 -0500 @@ -1,6 +1,6 @@ - +