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author | iuc |
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date | Fri, 04 Oct 2024 10:34:21 +0000 |
parents | f1dc2a761c30 |
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<tool id="fastq_combiner" name="Combine FASTA and QUAL" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>into FASTQ</description> <macros> <import>macros.xml</import> </macros> <edam_topics> <edam_topic>topic_0622</edam_topic> </edam_topics> <edam_operations> <edam_operation>operation_3436</edam_operation> </edam_operations> <expand macro="requirements"/> <command><![CDATA[ gx-fastq-combiner '$fasta_file' '${fasta_file.extension}' '$qual_file' '${qual_file.extension}' '$output_file' $force_quality_encoding ]]></command> <inputs> <param name="fasta_file" type="data" format="fasta,csfasta" label="FASTA File" /> <param name="qual_file" type="data" format="qual" optional="true" label="Quality Score File" /> <param name="force_quality_encoding" type="select" label="Force Quality Score encoding"> <option value="None">Use Source Encoding</option> <option value="ascii" selected="true">ASCII</option> <option value="decimal">Decimal</option> </param> </inputs> <outputs> <data name="output_file" format="fastqsanger"> <change_format> <when input="fasta_file" value="csfasta" format="fastqcssanger" /> <when input="qual_file" value="qualsolid" format="fastqcssanger" /> <when input="qual_file" value="qualsolexa" format="fastqsolexa" /> <when input="qual_file" value="qualillumina" format="fastqillumina" /> </change_format> </data> </outputs> <tests> <test> <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> <param name="qual_file" value="s2fq_phiX.qualsolid" ftype="qualsolid" /> <param name="force_quality_encoding" value="None" /> <output name="output_file" file="combine_phiX_out_1.fastqcssanger" /> </test> <test> <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> <param name="qual_file" value="s2fq_phiX.qualsolid" ftype="qualsolid" /> <param name="force_quality_encoding" value="ascii" /> <output name="output_file" file="combine_phiX_out_2.fastqcssanger" /> </test> <test> <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> <param name="qual_file" value="fastq_combiner_in_1.qual454" ftype="qual454" /> <param name="force_quality_encoding" value="None" /> <output name="output_file" file="wrapping_as_sanger.fastqsanger" /> </test> <test> <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> <param name="qual_file" value="fastq_combiner_in_1.qual454" ftype="qual454" /> <param name="force_quality_encoding" value="decimal" /> <output name="output_file" file="wrapping_as_sanger_decimal.fastqsanger" /> </test> <test> <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> <param name="qual_file" /> <param name="force_quality_encoding" value="decimal" /> <output name="output_file" file="fastq_combiner_no_qual_decimal_out_1.fastqsanger" /> </test> <test> <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> <param name="qual_file" /> <param name="force_quality_encoding" value="ascii" /> <output name="output_file" file="fastq_combiner_no_qual_ascii_out_1.fastqcssanger" /> </test> </tests> <help><![CDATA[ **What it does** This tool joins a FASTA file to a Quality Score file, creating a single FASTQ block for each read. Specifying a set of quality scores is optional; when not provided, the output will be fastqsanger or fastqcssanger (when a csfasta is provided) with each quality score being the maximal allowed value (93). Use this tool, for example, to convert 454-type output to FASTQ. ]]></help> <citations> <citation type="doi">10.1093/bioinformatics/btq281</citation> </citations> </tool>