Mercurial > repos > devteam > fastq_paired_end_splitter
comparison fastq_paired_end_splitter.py @ 0:c549e99026db draft
Imported from capsule None
author | devteam |
---|---|
date | Mon, 27 Jan 2014 09:29:20 -0500 |
parents | |
children |
comparison
equal
deleted
inserted
replaced
-1:000000000000 | 0:c549e99026db |
---|---|
1 #Dan Blankenberg | |
2 import sys, os, shutil | |
3 from galaxy_utils.sequence.fastq import fastqReader, fastqWriter, fastqSplitter | |
4 | |
5 def main(): | |
6 #Read command line arguments | |
7 input_filename = sys.argv[1] | |
8 input_type = sys.argv[2] or 'sanger' | |
9 output1_filename = sys.argv[3] | |
10 output2_filename = sys.argv[4] | |
11 | |
12 splitter = fastqSplitter() | |
13 out1 = fastqWriter( open( output1_filename, 'wb' ), format = input_type ) | |
14 out2 = fastqWriter( open( output2_filename, 'wb' ), format = input_type ) | |
15 | |
16 i = None | |
17 skip_count = 0 | |
18 for i, fastq_read in enumerate( fastqReader( open( input_filename, 'rb' ), format = input_type ) ): | |
19 read1, read2 = splitter.split( fastq_read ) | |
20 if read1 and read2: | |
21 out1.write( read1 ) | |
22 out2.write( read2 ) | |
23 else: | |
24 skip_count += 1 | |
25 out1.close() | |
26 out2.close() | |
27 if i is None: | |
28 print "Your file contains no valid FASTQ reads." | |
29 else: | |
30 print 'Split %s of %s reads (%.2f%%).' % ( i - skip_count + 1, i + 1, float( i - skip_count + 1 ) / float( i + 1 ) * 100.0 ) | |
31 | |
32 if __name__ == "__main__": | |
33 main() |