comparison fastq_to_tabular.py @ 0:bc9269529e88 draft

Imported from capsule None
author devteam
date Mon, 27 Jan 2014 09:28:21 -0500
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-1:000000000000 0:bc9269529e88
1 #Dan Blankenberg
2 import sys
3 from galaxy_utils.sequence.fastq import fastqReader
4
5 def stop_err( msg ):
6 sys.stderr.write( msg )
7 sys.exit()
8
9 def main():
10 if len(sys.argv) != 5:
11 stop_err("Wrong number of arguments. Expect: fasta tabular desrc_split [type]")
12 input_filename = sys.argv[1]
13 output_filename = sys.argv[2]
14 descr_split = int( sys.argv[3] ) - 1
15 if descr_split < 0:
16 stop_err("Bad description split value (should be 1 or more)")
17 input_type = sys.argv[4] or 'sanger' #input type should ordinarily be unnecessary
18
19 num_reads = None
20 fastq_read = None
21 out = open( output_filename, 'wb' )
22 if descr_split == 0:
23 #Don't divide the description into multiple columns
24 for num_reads, fastq_read in enumerate( fastqReader( open( input_filename ), format = input_type ) ):
25 out.write( "%s\t%s\t%s\n" % ( fastq_read.identifier[1:].replace( '\t', ' ' ), fastq_read.sequence.replace( '\t', ' ' ), fastq_read.quality.replace( '\t', ' ' ) ) )
26 else:
27 for num_reads, fastq_read in enumerate( fastqReader( open( input_filename ), format = input_type ) ):
28 words = fastq_read.identifier[1:].replace( '\t', ' ' ).split(None, descr_split)
29 #pad with empty columns if required
30 words += [""]*(descr_split-len(words))
31 out.write( "%s\t%s\t%s\n" % ("\t".join(words), fastq_read.sequence.replace( '\t', ' ' ), fastq_read.quality.replace( '\t', ' ' ) ) )
32 out.close()
33 if num_reads is None:
34 print "No valid FASTQ reads could be processed."
35 else:
36 print "%i FASTQ reads were converted to Tabular." % ( num_reads + 1 )
37
38 if __name__ == "__main__": main()