Mercurial > repos > devteam > fastqc
diff test-data/fastqc_data_adapters.txt @ 18:3e1cdf5406db draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 8a5d4f0470a7778a87d01dc84578f94a30ab7f43
| author | iuc |
|---|---|
| date | Mon, 05 Nov 2018 12:08:29 -0500 |
| parents | |
| children | 3d0c7bdf12f5 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastqc_data_adapters.txt Mon Nov 05 12:08:29 2018 -0500 @@ -0,0 +1,417 @@ +##FastQC 0.11.8 +>>Basic Statistics pass +#Measure Value +Filename 1000trimmed_fastq +File type Conventional base calls +Encoding Sanger / Illumina 1.9 +Total Sequences 4905 +Sequences flagged as poor quality 0 +Sequence length 1-108 +%GC 40 +>>END_MODULE +>>Per base sequence quality pass +#Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile +1 29.282772680937818 31.0 27.0 33.0 23.0 34.0 +2 28.832925336597306 30.0 27.0 32.0 22.0 33.0 +3 28.896953588223266 30.0 27.0 32.0 23.0 34.0 +4 29.006785934608267 30.0 27.0 32.0 23.0 34.0 +5 28.9377990430622 30.0 27.0 32.0 22.0 34.0 +6 29.002938706968934 30.0 27.0 32.0 23.0 34.0 +7 28.899830220713074 30.0 27.0 32.0 22.0 34.0 +8 28.900706184463942 30.0 27.0 32.0 22.0 33.0 +9 28.754602555772145 30.0 27.0 32.0 22.0 34.0 +10-11 28.822083022456294 30.0 27.0 32.0 22.5 33.0 +12-13 28.74898578710789 30.0 26.5 32.0 22.0 33.0 +14-15 28.53370750727077 30.0 26.0 32.0 22.0 33.5 +16-17 28.52661127583153 30.0 26.0 32.0 22.0 33.0 +18-19 28.68343192244741 30.0 26.0 32.0 22.5 33.0 +20-21 28.370130166336985 29.5 26.0 32.0 22.0 33.0 +22-23 28.185946266098476 29.0 26.0 32.0 22.0 33.0 +24-25 28.13009469533431 29.0 26.0 32.0 21.5 33.0 +26-27 28.075768267577 29.0 26.0 32.0 21.5 33.0 +28-29 27.828024188665616 29.0 25.0 32.0 21.0 33.0 +30-31 27.792826876513317 29.0 25.0 31.5 21.0 33.0 +32-33 27.490989073616355 28.0 25.0 31.0 21.0 33.0 +34-35 27.357199679261562 28.0 24.5 31.0 21.0 33.0 +36-37 27.062158129410527 28.0 24.0 31.0 20.5 33.0 +38-39 27.238022216783758 28.0 24.0 31.0 21.0 33.0 +40-41 27.028963434226593 28.0 24.0 31.0 20.5 33.0 +42-43 26.90862078496675 27.0 24.0 31.0 20.5 33.0 +44-45 26.632003369751054 27.0 24.0 30.0 20.0 32.0 +46-47 26.83042786373109 27.5 24.0 31.0 20.5 32.0 +48-49 26.431681832255798 27.0 23.5 30.0 20.0 32.0 +50-51 26.219000735104444 27.0 23.0 30.0 20.0 32.0 +52-53 25.925690423864786 26.5 22.5 29.5 19.5 31.5 +54-55 27.995241016389798 29.5 25.5 31.5 20.5 33.0 +56-57 30.410857897242785 31.5 28.5 33.0 25.5 34.0 +58-59 30.4083885209713 31.5 28.5 33.0 25.5 34.0 +60-61 30.444812362030905 31.5 29.0 33.0 26.0 34.0 +62-63 30.44039735099338 31.5 29.0 33.0 25.5 34.0 +64-65 30.441712697003883 31.5 29.0 33.0 25.5 34.0 +66-67 30.51141384388807 32.0 29.0 33.0 25.5 34.0 +68-69 30.05117820324006 31.0 28.0 33.0 24.5 34.0 +70-71 30.276141384388808 31.0 29.0 33.0 25.0 34.0 +72-73 30.385493372606774 31.5 29.0 33.0 25.5 34.0 +74-75 30.213549337260677 31.0 28.5 33.0 25.5 34.0 +76-77 29.678939617083948 31.0 28.0 33.0 24.5 34.0 +78-79 30.019145802650957 31.0 28.0 33.0 24.5 34.0 +80-81 29.664212076583212 31.0 27.5 33.0 24.0 34.0 +82-83 29.653534609720175 31.0 28.0 32.5 24.5 34.0 +84-85 29.425625920471283 30.5 27.0 32.0 24.0 34.0 +86-87 29.34057437407953 30.0 27.0 32.5 24.0 34.0 +88-89 28.785714285714285 30.0 27.0 32.0 22.5 33.5 +90-91 28.667525773195877 29.5 26.0 32.0 23.0 33.0 +92-93 28.565905743740796 29.0 26.0 32.0 23.0 33.0 +94-95 28.271723122238583 29.0 26.0 32.0 22.5 33.0 +96-97 27.94955817378498 29.0 25.5 31.0 22.0 33.0 +98-99 27.641132075471695 28.0 25.0 31.0 21.5 33.0 +100-101 27.614716981132077 28.0 25.0 31.0 21.5 33.0 +102-103 27.196226415094337 28.0 24.5 31.0 21.0 32.5 +104-105 26.870943396226416 27.5 24.0 31.0 20.0 32.5 +106-107 26.367593937711003 27.0 23.5 30.0 20.5 32.0 +108 27.5650623885918 28.0 24.0 31.0 22.0 33.0 +>>END_MODULE +>>Per sequence quality scores warn +#Quality Count +20 7.0 +21 24.0 +22 47.0 +23 78.0 +24 226.0 +25 513.0 +26 830.0 +27 1017.0 +28 947.0 +29 645.0 +30 352.0 +31 157.0 +32 55.0 +33 6.0 +34 1.0 +>>END_MODULE +>>Per base sequence content pass +#Base G A T C +1 21.223241590214066 33.00713557594291 25.97349643221203 19.79612640163099 +2 23.76580987352101 31.415748674010608 27.315381476948186 17.503059975520195 +3 21.876916785933346 28.70578613780413 29.46227765283173 19.95501942343079 +4 21.55048324079786 28.295290972650626 28.850503804236066 21.303721982315444 +5 21.219055543998337 30.205949656750576 28.271271063033076 20.303723736218014 +6 22.124265323257767 28.946263643996637 26.364399664147776 22.565071368597817 +7 21.943972835314092 29.9660441426146 28.395585738539896 19.69439728353141 +8 21.378129681147016 28.825165846351382 27.8621870318853 21.934517440616304 +9 20.077972709551656 29.088152479965345 28.654970760233915 22.17890405024908 +10-11 21.243237275035884 29.468919068124105 27.680247322512976 21.607596334327038 +12-13 20.8276950834461 29.03698691926026 29.18358141632837 20.951736580965267 +14-15 20.24001846295869 28.975305792753286 29.471497807523654 21.313177936764365 +16-17 19.83872880351002 29.396418830783826 29.621724178821296 21.14312818688486 +18-19 20.845552297165202 27.871456500488755 29.973118279569892 21.309872922776147 +20-21 20.538172715894866 28.973717146433042 29.111389236545683 21.376720901126408 +22-23 20.88082901554404 30.129533678756477 29.300518134715027 19.689119170984455 +24-25 20.506974248927037 29.07725321888412 29.05042918454936 21.365343347639485 +26-27 20.86603139013453 28.054932735426007 30.339125560538115 20.739910313901344 +28-29 19.32600732600733 30.842490842490843 28.776556776556777 21.054945054945055 +30-31 20.638820638820636 29.19226044226044 29.49938574938575 20.669533169533167 +32-33 19.47989016314004 29.009853012437407 29.38136003876595 22.1288967856566 +34-35 19.6674020023757 29.713219073477003 30.205328355676226 20.41405056847107 +36-37 20.443482963764197 29.853975121687398 30.61114115738237 19.091400757166035 +38-39 20.992661259173424 28.041714947856317 30.088837388953266 20.876786404016993 +40-41 19.860197368421055 28.680098684210524 30.32483552631579 21.134868421052634 +42-43 20.01321003963012 27.873183619550858 30.823425803610743 21.29018053720828 +44-45 18.941371943982908 30.192262046047947 29.954901495371466 20.911464514597675 +46-47 20.1888718734048 28.126595201633485 31.470137825421133 20.21439509954058 +48-49 19.56159822419534 29.689234184239734 28.77358490566038 21.975582685904552 +50-51 20.50656087885261 28.2575526396094 30.759841318278912 20.47604516325908 +52-53 17.691256830601095 29.918032786885245 31.864754098360653 20.525956284153004 +54-55 17.214912280701753 32.30994152046784 29.53216374269006 20.942982456140353 +56-57 19.30856932695844 30.893710923133504 30.893710923133504 18.90400882677455 +58-59 20.014716703458426 29.72774098601913 31.42016188373804 18.8373804267844 +60-61 17.402501839587934 33.18616629874908 29.506990434142754 19.904341427520237 +62-63 18.543046357615893 29.72774098601913 32.2663723325975 19.462840323767477 +64-65 16.893632683106365 32.94074346705926 30.180345969819655 19.985277880014724 +66-67 18.519882179675996 28.902798232695137 32.80559646539028 19.771723122238587 +68-69 17.2680412371134 30.375552282768776 31.66421207658321 20.692194403534607 +70-71 17.673048600883654 27.798232695139912 35.34609720176731 19.182621502209134 +72-73 16.200294550810014 29.528718703976438 33.87334315169367 20.39764359351988 +74-75 18.372606774668633 28.019145802650957 34.5360824742268 19.072164948453608 +76-77 16.678939617083945 31.70103092783505 31.73784977908689 19.88217967599411 +78-79 18.77761413843888 28.387334315169365 32.62150220913107 20.213549337260677 +80-81 16.384388807069218 30.927835051546392 32.9160530191458 19.771723122238587 +82-83 17.083946980854197 30.927835051546392 32.54786450662739 19.44035346097202 +84-85 16.936671575846834 31.590574374079527 31.443298969072163 20.029455081001473 +86-87 17.488954344624446 27.908689248895435 34.20471281296024 20.39764359351988 +88-89 16.789396170839467 32.36377025036819 31.075110456553755 19.771723122238587 +90-91 19.587628865979383 29.749631811487482 33.5419734904271 17.120765832106038 +92-93 17.746686303387335 30.04418262150221 31.33284241531664 20.876288659793815 +94-95 18.924889543446245 28.460972017673047 33.39469808541973 19.219440353460975 +96-97 16.42120765832106 29.418262150220915 32.47422680412371 21.686303387334316 +98-99 18.158090976882924 29.34377330350485 33.1469052945563 19.351230425055927 +100-101 17.77358490566038 29.849056603773583 33.28301886792453 19.09433962264151 +102-103 18.339622641509436 29.509433962264154 30.90566037735849 21.245283018867926 +104-105 18.037735849056606 28.67924528301887 31.77358490566038 21.50943396226415 +106-107 17.2782874617737 29.357798165137616 33.52446483180428 19.839449541284402 +108 20.053475935828878 26.38146167557932 31.72905525846702 21.836007130124777 +>>END_MODULE +>>Per sequence GC content warn +#GC Content Count +0 15.0 +1 15.5 +2 16.5 +3 17.0 +4 18.0 +5 21.5 +6 26.5 +7 30.0 +8 33.5 +9 36.0 +10 41.0 +11 47.0 +12 47.5 +13 56.0 +14 65.5 +15 69.0 +16 72.5 +17 78.0 +18 86.5 +19 94.5 +20 105.5 +21 113.0 +22 123.0 +23 132.5 +24 149.5 +25 172.5 +26 200.0 +27 222.0 +28 247.0 +29 282.0 +30 310.5 +31 337.0 +32 370.0 +33 403.5 +34 438.0 +35 465.0 +36 481.5 +37 511.0 +38 523.5 +39 506.0 +40 491.0 +41 491.5 +42 482.0 +43 483.0 +44 487.5 +45 471.5 +46 471.0 +47 468.5 +48 473.5 +49 472.0 +50 437.5 +51 415.5 +52 407.5 +53 398.0 +54 381.0 +55 364.0 +56 349.0 +57 341.0 +58 330.5 +59 320.0 +60 320.5 +61 301.0 +62 275.0 +63 245.0 +64 207.0 +65 190.5 +66 182.0 +67 173.0 +68 167.0 +69 151.5 +70 131.5 +71 121.0 +72 117.5 +73 110.5 +74 104.0 +75 90.5 +76 75.0 +77 67.5 +78 62.5 +79 61.5 +80 59.5 +81 57.0 +82 54.5 +83 47.0 +84 39.5 +85 38.0 +86 36.5 +87 35.5 +88 28.0 +89 21.0 +90 19.5 +91 17.0 +92 15.5 +93 14.5 +94 13.5 +95 13.5 +96 15.0 +97 15.5 +98 17.0 +99 16.0 +100 13.5 +>>END_MODULE +>>Per base N content pass +#Base N-Count +1 0.0 +2 0.0 +3 0.0 +4 0.0 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +10-11 0.0 +12-13 0.0 +14-15 0.0 +16-17 0.0 +18-19 0.0 +20-21 0.0 +22-23 0.0 +24-25 0.0 +26-27 0.0 +28-29 0.0 +30-31 0.0 +32-33 0.0 +34-35 0.0 +36-37 0.0 +38-39 0.0 +40-41 0.0 +42-43 0.0 +44-45 0.0 +46-47 0.0 +48-49 0.0 +50-51 0.0 +52-53 0.0 +54-55 0.0 +56-57 0.0 +58-59 0.0 +60-61 0.0 +62-63 0.0 +64-65 0.0 +66-67 0.0 +68-69 0.0 +70-71 0.0 +72-73 0.0 +74-75 0.0 +76-77 0.0 +78-79 0.0 +80-81 0.0 +82-83 0.0 +84-85 0.0 +86-87 0.0 +88-89 0.0 +90-91 0.0 +92-93 0.0 +94-95 0.0 +96-97 0.0 +98-99 0.0 +100-101 0.0 +102-103 0.0 +104-105 0.0 +106-107 0.0 +108 0.0 +>>END_MODULE +>>Sequence Length Distribution warn +#Length Count +0-4 98.0 +5-9 250.0 +10-14 257.0 +15-19 279.0 +20-24 321.0 +25-29 396.0 +30-34 401.0 +35-39 433.0 +40-44 401.0 +45-49 385.0 +50-54 320.0 +55-59 5.0 +60-64 1.0 +65-69 0.0 +70-74 0.0 +75-79 0.0 +80-84 0.0 +85-89 0.0 +90-94 0.0 +95-99 33.0 +100-104 0.0 +105-109 1325.0 +>>END_MODULE +>>Sequence Duplication Levels pass +#Total Deduplicated Percentage 98.73598369011212 +#Duplication Level Percentage of deduplicated Percentage of total +1 99.44249432170142 98.1855249745158 +2 0.47491224447656405 0.9378185524974516 +3 0.041296716911005574 0.12232415902140673 +4 0.020648358455502787 0.08154943934760449 +5 0.0 0.0 +6 0.0 0.0 +7 0.0 0.0 +8 0.0 0.0 +9 0.0 0.0 +>10 0.020648358455502787 0.672782874617737 +>50 0.0 0.0 +>100 0.0 0.0 +>500 0.0 0.0 +>1k 0.0 0.0 +>5k 0.0 0.0 +>10k+ 0.0 0.0 +>>END_MODULE +>>Overrepresented sequences warn +#Sequence Count Percentage Possible Source +ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit +>>END_MODULE +>>Adapter Content pass +#Position Illumina Single End Apapter 1 Illumina Single End Apapter 2 Illumina Single End PCR Primer 1 Illumina Single End PCR Primer 2 Illumina Single End Sequencing Primer Illumina Paired End Adapter 1 Illumina Paired End Adapter 2 Illumina Paried End PCR Primer 1 Illumina Paired End PCR Primer 2 Illumina Paried End Sequencing Primer 1 Illumina Paired End Sequencing Primer 2 Illumina DpnII expression Adapter 1 Illumina DpnII expression Adapter 2 Illumina DpnII expression PCR Primer 1 Illumina DpnII expression PCR Primer 2 Illumina DpnII expression Sequencing Primer Illumina NlaIII expression Adapter 1 Illumina NlaIII expression Adapter 2 Illumina NlaIII expression PCR Primer 1 Illumina NlaIII expression PCR Primer 2 Illumina NlaIII expression Sequencing Primer Illumina Small RNA Adapter 1 Illumina Small RNA Adapter 2 Illumina Small RNA RT Primer Illumina Small RNA PCR Primer 1 Illumina Small RNA PCR Primer 2 Illumina Small RNA Sequencing Primer Illumina Multiplexing Adapter 1 Illumina Multiplexing Adapter 2 Illumina Multiplexing PCR Primer 1.01 Illumina Multiplexing PCR Primer 2.01 Illumina Multiplexing Read1 Sequencing Primer Illumina Multiplexing Index Sequencing Primer Illumina Multiplexing Read2 Sequencing Primer Illumina PCR Primer Index 1 Illumina PCR Primer Index 2 Illumina PCR Primer Index 3 Illumina PCR Primer Index 4 Illumina PCR Primer Index 5 Illumina PCR Primer Index 6 Illumina PCR Primer Index 7 Illumina PCR Primer Index 8 Illumina PCR Primer Index 9 Illumina PCR Primer Index 10 Illumina PCR Primer Index 11 Illumina PCR Primer Index 12 Illumina DpnII Gex Adapter 1 Illumina DpnII Gex Adapter 1.01 Illumina DpnII Gex Adapter 2 Illumina DpnII Gex Adapter 2.01 Illumina DpnII Gex PCR Primer 1 Illumina DpnII Gex PCR Primer 2 Illumina DpnII Gex Sequencing Primer Illumina NlaIII Gex Adapter 1.01 Illumina NlaIII Gex Adapter 1.02 Illumina NlaIII Gex Adapter 2.01 Illumina NlaIII Gex Adapter 2.02 Illumina NlaIII Gex PCR Primer 1 Illumina NlaIII Gex PCR Primer 2 Illumina NlaIII Gex Sequencing Primer Illumina Small RNA RT Primer Illumina 5p RNA Adapter Illumina RNA Adapter1 Illumina Small RNA 3p Adapter 1 Illumina Small RNA PCR Primer 1 Illumina Small RNA PCR Primer 2 Illumina Small RNA Sequencing Primer TruSeq Universal Adapter TruSeq Adapter, Index 1 TruSeq Adapter, Index 2 TruSeq Adapter, Index 3 TruSeq Adapter, Index 4 TruSeq Adapter, Index 5 TruSeq Adapter, Index 6 TruSeq Adapter, Index 7 TruSeq Adapter, Index 8 TruSeq Adapter, Index 9 TruSeq Adapter, Index 10 TruSeq Adapter, Index 11 TruSeq Adapter, Index 12 Illumina RNA RT Primer Illumina RNA PCR Primer RNA PCR Primer, Index 1 RNA PCR Primer, Index 2 RNA PCR Primer, Index 3 RNA PCR Primer, Index 4 RNA PCR Primer, Index 5 RNA PCR Primer, Index 6 RNA PCR Primer, Index 7 RNA PCR Primer, Index 8 RNA PCR Primer, Index 9 RNA PCR Primer, Index 10 RNA PCR Primer, Index 11 RNA PCR Primer, Index 12 RNA PCR Primer, Index 13 RNA PCR Primer, Index 14 RNA PCR Primer, Index 15 RNA PCR Primer, Index 16 RNA PCR Primer, Index 17 RNA PCR Primer, Index 18 RNA PCR Primer, Index 19 RNA PCR Primer, Index 20 RNA PCR Primer, Index 21 RNA PCR Primer, Index 22 RNA PCR Primer, Index 23 RNA PCR Primer, Index 24 RNA PCR Primer, Index 25 RNA PCR Primer, Index 26 RNA PCR Primer, Index 27 RNA PCR Primer, Index 28 RNA PCR Primer, Index 29 RNA PCR Primer, Index 30 RNA PCR Primer, Index 31 RNA PCR Primer, Index 32 RNA PCR Primer, Index 33 RNA PCR Primer, Index 34 RNA PCR Primer, Index 35 RNA PCR Primer, Index 36 RNA PCR Primer, Index 37 RNA PCR Primer, Index 38 RNA PCR Primer, Index 39 RNA PCR Primer, Index 40 RNA PCR Primer, Index 41 RNA PCR Primer, Index 42 RNA PCR Primer, Index 43 RNA PCR Primer, Index 44 RNA PCR Primer, Index 45 RNA PCR Primer, Index 46 RNA PCR Primer, Index 47 RNA PCR Primer, Index 48 ABI Dynabead EcoP Oligo ABI Solid3 Adapter A ABI Solid3 Adapter B ABI Solid3 5' AMP Primer ABI Solid3 3' AMP Primer ABI Solid3 EF1 alpha Sense Primer ABI Solid3 EF1 alpha Antisense Primer ABI Solid3 GAPDH Forward Primer ABI Solid3 GAPDH Reverse Primer +1 0.020387359836901122 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 +2 0.040774719673802244 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 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