Mercurial > repos > devteam > fastqc
diff test-data/fastqc_data.txt @ 1:8fae48caaf06 draft
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author | devteam |
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date | Tue, 11 Nov 2014 12:46:27 -0500 |
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children | 3fdc1a74d866 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastqc_data.txt Tue Nov 11 12:46:27 2014 -0500 @@ -0,0 +1,934 @@ +##FastQC 0.11.2 +>>Basic Statistics pass +#Measure Value +Filename 1000gsample.fastq +File type Conventional base calls +Encoding Sanger / Illumina 1.9 +Total Sequences 5000 +Sequences flagged as poor quality 0 +Sequence length 54 +%GC 43 +>>END_MODULE +>>Per base sequence quality warn +#Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile +1 28.9946 31.0 27.0 33.0 22.0 34.0 +2 28.486 30.0 26.0 32.0 21.0 33.0 +3 28.5272 30.0 26.0 32.0 21.0 34.0 +4 28.6252 30.0 26.0 32.0 22.0 33.0 +5 28.4804 30.0 26.0 32.0 21.0 33.0 +6 28.5128 30.0 26.0 32.0 22.0 33.0 +7 28.4102 30.0 26.0 32.0 21.0 33.0 +8 28.4674 30.0 26.0 32.0 22.0 33.0 +9 28.182 30.0 26.0 32.0 21.0 33.0 +10 28.0908 30.0 26.0 32.0 21.0 33.0 +11 28.1876 30.0 26.0 32.0 21.0 33.0 +12 28.1742 30.0 26.0 32.0 21.0 33.0 +13 27.923 30.0 25.0 32.0 20.0 33.0 +14 27.8984 29.0 25.0 32.0 21.0 33.0 +15 27.6112 29.0 25.0 32.0 20.0 33.0 +16 27.6638 29.0 25.0 32.0 20.0 33.0 +17 27.5734 29.0 25.0 32.0 20.0 33.0 +18 27.6348 29.0 25.0 32.0 20.0 33.0 +19 27.6526 29.0 25.0 32.0 20.0 33.0 +20 27.2716 29.0 24.0 32.0 19.0 33.0 +21 27.2642 29.0 25.0 31.0 19.0 33.0 +22 26.881 28.0 24.0 31.0 19.0 33.0 +23 26.8124 28.0 24.0 31.0 19.0 33.0 +24 26.8204 28.0 24.0 31.0 19.0 33.0 +25 26.6606 28.0 24.0 31.0 18.0 33.0 +26 26.3806 28.0 23.0 31.0 18.0 33.0 +27 25.947 28.0 23.0 31.0 17.0 33.0 +28 25.7446 27.0 22.0 31.0 16.0 33.0 +29 25.6856 27.0 22.0 31.0 16.0 33.0 +30 25.5228 27.0 22.0 31.0 16.0 33.0 +31 25.354 27.0 22.0 30.0 16.0 32.0 +32 25.1656 27.0 22.0 30.0 16.0 32.0 +33 25.0164 26.0 21.0 30.0 15.0 32.0 +34 24.75 26.0 21.0 30.0 15.0 32.0 +35 24.3926 26.0 21.0 30.0 15.0 32.0 +36 24.1122 25.0 20.0 30.0 14.0 32.0 +37 23.8612 25.0 20.0 29.0 13.0 32.0 +38 23.7544 25.0 20.0 29.0 13.0 32.0 +39 23.485 25.0 19.0 29.0 13.0 32.0 +40 23.1984 24.0 19.0 29.0 13.0 32.0 +41 23.0976 24.0 19.0 29.0 13.0 32.0 +42 22.9212 24.0 19.0 28.0 13.0 31.0 +43 22.5172 24.0 18.0 28.0 12.0 31.0 +44 22.3234 23.0 18.0 28.0 12.0 31.0 +45 22.1372 23.0 18.0 28.0 11.0 31.0 +46 22.2166 23.0 18.0 28.0 11.0 31.0 +47 22.075 23.0 18.0 28.0 11.0 31.0 +48 21.4592 22.0 17.0 27.0 11.0 31.0 +49 21.0954 22.0 16.0 27.0 10.0 30.0 +50 20.9356 22.0 16.0 27.0 10.0 30.0 +51 20.6152 21.0 15.0 27.0 9.0 30.0 +52 20.6588 21.0 16.0 27.0 9.0 30.0 +53 19.5852 20.0 14.0 25.0 9.0 29.0 +54 19.9424 21.0 14.0 26.0 7.0 30.0 +>>END_MODULE +>>Per tile sequence quality pass +#Tile Base Mean +1 1 0.4542485249902164 +1 2 0.02493171505959424 +1 3 0.004493134032774293 +1 4 0.6119404948560927 +1 5 0.5283275008594686 +1 6 0.3741933003058371 +1 7 -0.2985554485298749 +1 8 0.6040181642739206 +1 9 0.43795745033596845 +1 10 0.05327706110314523 +1 11 0.8097461135057031 +1 12 0.9171964750225641 +1 13 1.0716973449965757 +1 14 0.562649938864773 +1 15 0.5112535943737946 +1 16 0.7098461441939676 +1 17 0.3989886413671577 +1 18 0.2351551176870892 +1 19 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3.2918952034041524 +10 41 1.3115749098869323 +10 42 2.911702897841007 +10 43 2.9292302270051636 +10 44 1.964400342482186 +10 45 0.5477757053971892 +10 46 1.9135298205630704 +10 47 1.2741568491184836 +10 48 2.799334226444202 +10 49 3.441999390209105 +10 50 4.391043406721156 +10 51 3.7815999232367545 +10 52 1.3217607371315836 +10 53 3.0125314696670173 +10 54 2.2316249763308633 +>>END_MODULE +>>Per sequence quality scores warn +#Quality Count +8 4.0 +9 4.0 +10 2.0 +11 2.0 +12 4.0 +13 6.0 +14 4.0 +15 11.0 +16 20.0 +17 50.0 +18 98.0 +19 149.0 +20 210.0 +21 275.0 +22 367.0 +23 425.0 +24 518.0 +25 540.0 +26 539.0 +27 532.0 +28 468.0 +29 360.0 +30 234.0 +31 122.0 +32 51.0 +33 5.0 +>>END_MODULE +>>Per base sequence content warn +#Base G A T C +1 21.5 32.64 25.919999999999998 19.939999999999998 +2 24.04 31.72 26.540000000000003 17.7 +3 21.58 28.860000000000003 29.42 20.14 +4 21.58 28.28 28.9 21.240000000000002 +5 21.16 29.720000000000002 28.32 20.8 +6 22.38 29.24 25.72 22.66 +7 21.34 30.320000000000004 28.24 20.1 +8 21.58 29.060000000000002 27.939999999999998 21.42 +9 20.3 28.48 28.58 22.64 +10 21.2 30.84 26.56 21.4 +11 21.14 28.64 27.279999999999998 22.939999999999998 +12 21.32 28.88 28.999999999999996 20.8 +13 20.36 29.220000000000002 28.939999999999998 21.48 +14 20.02 29.2 29.220000000000002 21.560000000000002 +15 20.200000000000003 29.220000000000002 28.38 22.2 +16 20.34 29.38 29.299999999999997 20.979999999999997 +17 20.599999999999998 29.14 28.499999999999996 21.759999999999998 +18 20.599999999999998 27.92 29.4 22.08 +19 21.66 28.46 28.58 21.3 +20 20.0 29.24 28.96 21.8 +21 21.72 28.499999999999996 28.18 21.6 +22 20.52 30.880000000000003 27.3 21.3 +23 20.72 28.9 29.720000000000002 20.66 +24 21.12 28.62 27.58 22.68 +25 21.099999999999998 29.060000000000002 29.160000000000004 20.68 +26 22.18887555022009 28.391356542617046 28.131252501000397 21.288515406162464 +27 21.344268853770753 28.525705141028208 28.765753150630125 21.364272854570913 +28 19.34 30.54 27.46 22.66 +29 20.90418083616723 29.12582516503301 29.045809161832363 20.924184836967395 +30 21.099999999999998 29.4 27.439999999999998 22.06 +31 20.84 28.04 28.660000000000004 22.46 +32 19.847939175670266 28.371348539415763 28.7515006002401 23.02921168467387 +33 22.084416883376676 28.62572514502901 27.265453090618124 22.024404880976196 +34 21.304260852170433 29.285857171434287 27.265453090618124 22.144428885777156 +35 21.084216843368676 29.425885177035404 28.225645129025807 21.264252850570113 +36 21.404280856171233 28.665733146629325 28.805761152230446 21.124224844968996 +37 20.888355342136855 29.43177270908363 28.53141256502601 21.1484593837535 +38 21.864372874574915 29.02580516103221 28.065613122624526 21.044208841768352 +39 21.928771508603443 28.251300520208083 27.050820328131252 22.769107643057225 +40 21.32 28.499999999999996 27.82 22.36 +41 21.673003802281368 28.116870122073244 27.61656994196518 22.59355613368021 +42 22.29337602561537 28.036822093255953 27.71662997798679 21.953171903141886 +43 21.244248849769953 28.425685137027408 27.825565113022606 22.504500900180034 +44 21.404280856171233 28.985797159431886 27.545509101820365 22.064412882576516 +45 21.86 29.28 26.740000000000002 22.12 +46 21.26 27.54 27.74 23.46 +47 21.628651460584233 28.271308523409367 28.051220488195277 22.048819527811126 +48 21.279999999999998 28.439999999999998 26.400000000000002 23.880000000000003 +49 21.044208841768352 29.045809161832363 27.185437087417487 22.724544908981798 +50 21.82873149259704 28.15126050420168 26.810724289715886 23.209283713485394 +51 21.028411364545818 27.73109243697479 28.011204481792717 23.229291716686674 +52 21.824364872974595 30.146029205841167 25.94518903780756 22.084416883376676 +53 21.164232846569313 28.325665133026607 28.045609121824366 22.464492898579717 +54 21.53907976692787 27.024311834438418 26.90375728350412 24.5328511151296 +>>END_MODULE +>>Per sequence GC content warn +#GC Content Count +0 3.0 +1 5.0 +2 7.0 +3 7.0 +4 7.0 +5 5.0 +6 2.0 +7 1.0 +8 1.5 +9 2.0 +10 1.5 +11 1.0 +12 2.5 +13 4.0 +14 4.0 +15 4.0 +16 5.0 +17 6.0 +18 11.0 +19 19.5 +20 23.0 +21 37.5 +22 52.0 +23 61.0 +24 70.0 +25 79.0 +26 88.0 +27 117.5 +28 147.0 +29 159.5 +30 172.0 +31 203.0 +32 243.0 +33 252.0 +34 290.0 +35 328.0 +36 330.0 +37 332.0 +38 332.5 +39 333.0 +40 342.0 +41 351.0 +42 343.5 +43 336.0 +44 318.5 +45 279.5 +46 258.0 +47 254.5 +48 251.0 +49 234.5 +50 218.0 +51 209.5 +52 201.0 +53 198.5 +54 196.0 +55 177.0 +56 162.5 +57 167.0 +58 147.0 +59 127.0 +60 106.5 +61 86.0 +62 85.0 +63 84.0 +64 61.5 +65 39.0 +66 42.5 +67 46.0 +68 34.0 +69 17.0 +70 12.0 +71 14.0 +72 16.0 +73 12.5 +74 9.0 +75 6.5 +76 4.0 +77 4.5 +78 5.0 +79 5.0 +80 5.0 +81 3.0 +82 1.0 +83 1.0 +84 1.0 +85 1.0 +86 1.0 +87 1.0 +88 1.0 +89 1.0 +90 2.0 +91 3.0 +92 2.5 +93 2.0 +94 2.0 +95 3.0 +96 4.0 +97 5.0 +98 6.0 +99 3.5 +100 1.0 +>>END_MODULE +>>Per base N content pass +#Base N-Count +1 0.0 +2 0.0 +3 0.0 +4 0.0 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +10 0.0 +11 0.0 +12 0.0 +13 0.0 +14 0.0 +15 0.0 +16 0.0 +17 0.0 +18 0.0 +19 0.0 +20 0.0 +21 0.0 +22 0.0 +23 0.0 +24 0.0 +25 0.0 +26 0.04 +27 0.02 +28 0.0 +29 0.02 +30 0.0 +31 0.0 +32 0.04 +33 0.02 +34 0.02 +35 0.02 +36 0.02 +37 0.04 +38 0.02 +39 0.04 +40 0.0 +41 0.06 +42 0.06 +43 0.02 +44 0.02 +45 0.0 +46 0.0 +47 0.04 +48 0.0 +49 0.02 +50 0.04 +51 0.04 +52 0.02 +53 0.02 +54 0.45999999999999996 +>>END_MODULE +>>Sequence Length Distribution pass +#Length Count +54 5000.0 +>>END_MODULE +>>Sequence Duplication Levels pass +#Total Deduplicated Percentage 99.52 +#Duplication Level Percentage of deduplicated Percentage of total +1 99.81913183279742 99.33999999999999 +2 0.08038585209003216 0.16 +3 0.04019292604501608 0.12 +4 0.0 0.0 +5 0.02009646302250804 0.1 +6 0.0 0.0 +7 0.04019292604501608 0.27999999999999997 +8 0.0 0.0 +9 0.0 0.0 +>10 0.0 0.0 +>50 0.0 0.0 +>100 0.0 0.0 +>500 0.0 0.0 +>1k 0.0 0.0 +>5k 0.0 0.0 +>10k+ 0.0 0.0 +>>END_MODULE +>>Overrepresented sequences warn +#Sequence Count Percentage Possible Source +CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (96% over 33bp) +CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC 7 0.13999999999999999 Illumina Paired End PCR Primer 2 (100% over 35bp) +>>END_MODULE +>>Adapter Content pass +#Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence +1 0.0 0.0 0.0 +2 0.02 0.0 0.0 +3 0.02 0.0 0.0 +4 0.02 0.0 0.0 +5 0.02 0.0 0.0 +6 0.02 0.0 0.0 +7 0.02 0.0 0.0 +8 0.02 0.0 0.0 +9 0.02 0.0 0.0 +10 0.02 0.0 0.0 +11 0.02 0.0 0.0 +12 0.02 0.0 0.0 +13 0.02 0.0 0.0 +14 0.02 0.0 0.0 +15 0.02 0.0 0.0 +16 0.02 0.0 0.0 +17 0.02 0.0 0.0 +18 0.02 0.0 0.0 +19 0.06 0.0 0.0 +20 0.36 0.0 0.0 +21 0.36 0.0 0.0 +22 0.36 0.0 0.0 +23 0.36 0.0 0.0 +24 0.36 0.0 0.0 +25 0.4 0.0 0.0 +26 0.4 0.0 0.0 +27 0.42 0.0 0.0 +28 0.62 0.0 0.0 +29 0.66 0.0 0.0 +30 0.68 0.0 0.0 +31 0.68 0.0 0.0 +32 0.68 0.0 0.0 +33 0.68 0.0 0.0 +34 0.7 0.0 0.0 +35 0.76 0.0 0.0 +36 0.8 0.0 0.0 +37 0.88 0.0 0.0 +38 1.0 0.0 0.0 +39 1.12 0.0 0.0 +40 1.22 0.0 0.0 +41 1.38 0.0 0.0 +42 1.48 0.0 0.0 +>>END_MODULE +>>Kmer Content pass +>>END_MODULE