Mercurial > repos > devteam > fastqc
diff test-data/fastqc_data_customlimits.txt @ 20:ddf5c37952ac draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 89976e27bd11f1da3c95a5df6b67297496a8a89b
| author | iuc |
|---|---|
| date | Thu, 16 May 2019 07:45:56 -0400 |
| parents | 3e1cdf5406db |
| children | 3d0c7bdf12f5 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastqc_data_customlimits.txt Thu May 16 07:45:56 2019 -0400 @@ -0,0 +1,426 @@ +##FastQC 0.11.8 +>>Basic Statistics pass +#Measure Value +Filename 1000trimmed_fastq +File type Conventional base calls +Encoding Sanger / Illumina 1.9 +Total Sequences 4905 +Sequences flagged as poor quality 0 +Sequence length 1-108 +%GC 40 +>>END_MODULE +>>Per base sequence quality pass +#Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile +1 29.282772680937818 31.0 27.0 33.0 23.0 34.0 +2 28.832925336597306 30.0 27.0 32.0 22.0 33.0 +3 28.896953588223266 30.0 27.0 32.0 23.0 34.0 +4 29.006785934608267 30.0 27.0 32.0 23.0 34.0 +5 28.9377990430622 30.0 27.0 32.0 22.0 34.0 +6 29.002938706968934 30.0 27.0 32.0 23.0 34.0 +7 28.899830220713074 30.0 27.0 32.0 22.0 34.0 +8 28.900706184463942 30.0 27.0 32.0 22.0 33.0 +9 28.754602555772145 30.0 27.0 32.0 22.0 34.0 +10-11 28.822083022456294 30.0 27.0 32.0 22.5 33.0 +12-13 28.74898578710789 30.0 26.5 32.0 22.0 33.0 +14-15 28.53370750727077 30.0 26.0 32.0 22.0 33.5 +16-17 28.52661127583153 30.0 26.0 32.0 22.0 33.0 +18-19 28.68343192244741 30.0 26.0 32.0 22.5 33.0 +20-21 28.370130166336985 29.5 26.0 32.0 22.0 33.0 +22-23 28.185946266098476 29.0 26.0 32.0 22.0 33.0 +24-25 28.13009469533431 29.0 26.0 32.0 21.5 33.0 +26-27 28.075768267577 29.0 26.0 32.0 21.5 33.0 +28-29 27.828024188665616 29.0 25.0 32.0 21.0 33.0 +30-31 27.792826876513317 29.0 25.0 31.5 21.0 33.0 +32-33 27.490989073616355 28.0 25.0 31.0 21.0 33.0 +34-35 27.357199679261562 28.0 24.5 31.0 21.0 33.0 +36-37 27.062158129410527 28.0 24.0 31.0 20.5 33.0 +38-39 27.238022216783758 28.0 24.0 31.0 21.0 33.0 +40-41 27.028963434226593 28.0 24.0 31.0 20.5 33.0 +42-43 26.90862078496675 27.0 24.0 31.0 20.5 33.0 +44-45 26.632003369751054 27.0 24.0 30.0 20.0 32.0 +46-47 26.83042786373109 27.5 24.0 31.0 20.5 32.0 +48-49 26.431681832255798 27.0 23.5 30.0 20.0 32.0 +50-51 26.219000735104444 27.0 23.0 30.0 20.0 32.0 +52-53 25.925690423864786 26.5 22.5 29.5 19.5 31.5 +54-55 27.995241016389798 29.5 25.5 31.5 20.5 33.0 +56-57 30.410857897242785 31.5 28.5 33.0 25.5 34.0 +58-59 30.4083885209713 31.5 28.5 33.0 25.5 34.0 +60-61 30.444812362030905 31.5 29.0 33.0 26.0 34.0 +62-63 30.44039735099338 31.5 29.0 33.0 25.5 34.0 +64-65 30.441712697003883 31.5 29.0 33.0 25.5 34.0 +66-67 30.51141384388807 32.0 29.0 33.0 25.5 34.0 +68-69 30.05117820324006 31.0 28.0 33.0 24.5 34.0 +70-71 30.276141384388808 31.0 29.0 33.0 25.0 34.0 +72-73 30.385493372606774 31.5 29.0 33.0 25.5 34.0 +74-75 30.213549337260677 31.0 28.5 33.0 25.5 34.0 +76-77 29.678939617083948 31.0 28.0 33.0 24.5 34.0 +78-79 30.019145802650957 31.0 28.0 33.0 24.5 34.0 +80-81 29.664212076583212 31.0 27.5 33.0 24.0 34.0 +82-83 29.653534609720175 31.0 28.0 32.5 24.5 34.0 +84-85 29.425625920471283 30.5 27.0 32.0 24.0 34.0 +86-87 29.34057437407953 30.0 27.0 32.5 24.0 34.0 +88-89 28.785714285714285 30.0 27.0 32.0 22.5 33.5 +90-91 28.667525773195877 29.5 26.0 32.0 23.0 33.0 +92-93 28.565905743740796 29.0 26.0 32.0 23.0 33.0 +94-95 28.271723122238583 29.0 26.0 32.0 22.5 33.0 +96-97 27.94955817378498 29.0 25.5 31.0 22.0 33.0 +98-99 27.641132075471695 28.0 25.0 31.0 21.5 33.0 +100-101 27.614716981132077 28.0 25.0 31.0 21.5 33.0 +102-103 27.196226415094337 28.0 24.5 31.0 21.0 32.5 +104-105 26.870943396226416 27.5 24.0 31.0 20.0 32.5 +106-107 26.367593937711003 27.0 23.5 30.0 20.5 32.0 +108 27.5650623885918 28.0 24.0 31.0 22.0 33.0 +>>END_MODULE +>>Per sequence quality scores warn +#Quality Count +20 7.0 +21 24.0 +22 47.0 +23 78.0 +24 226.0 +25 513.0 +26 830.0 +27 1017.0 +28 947.0 +29 645.0 +30 352.0 +31 157.0 +32 55.0 +33 6.0 +34 1.0 +>>END_MODULE +>>Per base sequence content pass +#Base G A T C +1 21.223241590214066 33.00713557594291 25.97349643221203 19.79612640163099 +2 23.76580987352101 31.415748674010608 27.315381476948186 17.503059975520195 +3 21.876916785933346 28.70578613780413 29.46227765283173 19.95501942343079 +4 21.55048324079786 28.295290972650626 28.850503804236066 21.303721982315444 +5 21.219055543998337 30.205949656750576 28.271271063033076 20.303723736218014 +6 22.124265323257767 28.946263643996637 26.364399664147776 22.565071368597817 +7 21.943972835314092 29.9660441426146 28.395585738539896 19.69439728353141 +8 21.378129681147016 28.825165846351382 27.8621870318853 21.934517440616304 +9 20.077972709551656 29.088152479965345 28.654970760233915 22.17890405024908 +10-11 21.243237275035884 29.468919068124105 27.680247322512976 21.607596334327038 +12-13 20.8276950834461 29.03698691926026 29.18358141632837 20.951736580965267 +14-15 20.24001846295869 28.975305792753286 29.471497807523654 21.313177936764365 +16-17 19.83872880351002 29.396418830783826 29.621724178821296 21.14312818688486 +18-19 20.845552297165202 27.871456500488755 29.973118279569892 21.309872922776147 +20-21 20.538172715894866 28.973717146433042 29.111389236545683 21.376720901126408 +22-23 20.88082901554404 30.129533678756477 29.300518134715027 19.689119170984455 +24-25 20.506974248927037 29.07725321888412 29.05042918454936 21.365343347639485 +26-27 20.86603139013453 28.054932735426007 30.339125560538115 20.739910313901344 +28-29 19.32600732600733 30.842490842490843 28.776556776556777 21.054945054945055 +30-31 20.638820638820636 29.19226044226044 29.49938574938575 20.669533169533167 +32-33 19.47989016314004 29.009853012437407 29.38136003876595 22.1288967856566 +34-35 19.6674020023757 29.713219073477003 30.205328355676226 20.41405056847107 +36-37 20.443482963764197 29.853975121687398 30.61114115738237 19.091400757166035 +38-39 20.992661259173424 28.041714947856317 30.088837388953266 20.876786404016993 +40-41 19.860197368421055 28.680098684210524 30.32483552631579 21.134868421052634 +42-43 20.01321003963012 27.873183619550858 30.823425803610743 21.29018053720828 +44-45 18.941371943982908 30.192262046047947 29.954901495371466 20.911464514597675 +46-47 20.1888718734048 28.126595201633485 31.470137825421133 20.21439509954058 +48-49 19.56159822419534 29.689234184239734 28.77358490566038 21.975582685904552 +50-51 20.50656087885261 28.2575526396094 30.759841318278912 20.47604516325908 +52-53 17.691256830601095 29.918032786885245 31.864754098360653 20.525956284153004 +54-55 17.214912280701753 32.30994152046784 29.53216374269006 20.942982456140353 +56-57 19.30856932695844 30.893710923133504 30.893710923133504 18.90400882677455 +58-59 20.014716703458426 29.72774098601913 31.42016188373804 18.8373804267844 +60-61 17.402501839587934 33.18616629874908 29.506990434142754 19.904341427520237 +62-63 18.543046357615893 29.72774098601913 32.2663723325975 19.462840323767477 +64-65 16.893632683106365 32.94074346705926 30.180345969819655 19.985277880014724 +66-67 18.519882179675996 28.902798232695137 32.80559646539028 19.771723122238587 +68-69 17.2680412371134 30.375552282768776 31.66421207658321 20.692194403534607 +70-71 17.673048600883654 27.798232695139912 35.34609720176731 19.182621502209134 +72-73 16.200294550810014 29.528718703976438 33.87334315169367 20.39764359351988 +74-75 18.372606774668633 28.019145802650957 34.5360824742268 19.072164948453608 +76-77 16.678939617083945 31.70103092783505 31.73784977908689 19.88217967599411 +78-79 18.77761413843888 28.387334315169365 32.62150220913107 20.213549337260677 +80-81 16.384388807069218 30.927835051546392 32.9160530191458 19.771723122238587 +82-83 17.083946980854197 30.927835051546392 32.54786450662739 19.44035346097202 +84-85 16.936671575846834 31.590574374079527 31.443298969072163 20.029455081001473 +86-87 17.488954344624446 27.908689248895435 34.20471281296024 20.39764359351988 +88-89 16.789396170839467 32.36377025036819 31.075110456553755 19.771723122238587 +90-91 19.587628865979383 29.749631811487482 33.5419734904271 17.120765832106038 +92-93 17.746686303387335 30.04418262150221 31.33284241531664 20.876288659793815 +94-95 18.924889543446245 28.460972017673047 33.39469808541973 19.219440353460975 +96-97 16.42120765832106 29.418262150220915 32.47422680412371 21.686303387334316 +98-99 18.158090976882924 29.34377330350485 33.1469052945563 19.351230425055927 +100-101 17.77358490566038 29.849056603773583 33.28301886792453 19.09433962264151 +102-103 18.339622641509436 29.509433962264154 30.90566037735849 21.245283018867926 +104-105 18.037735849056606 28.67924528301887 31.77358490566038 21.50943396226415 +106-107 17.2782874617737 29.357798165137616 33.52446483180428 19.839449541284402 +108 20.053475935828878 26.38146167557932 31.72905525846702 21.836007130124777 +>>END_MODULE +>>Per sequence GC content warn +#GC Content Count +0 15.0 +1 15.5 +2 16.5 +3 17.0 +4 18.0 +5 21.5 +6 26.5 +7 30.0 +8 33.5 +9 36.0 +10 41.0 +11 47.0 +12 47.5 +13 56.0 +14 65.5 +15 69.0 +16 72.5 +17 78.0 +18 86.5 +19 94.5 +20 105.5 +21 113.0 +22 123.0 +23 132.5 +24 149.5 +25 172.5 +26 200.0 +27 222.0 +28 247.0 +29 282.0 +30 310.5 +31 337.0 +32 370.0 +33 403.5 +34 438.0 +35 465.0 +36 481.5 +37 511.0 +38 523.5 +39 506.0 +40 491.0 +41 491.5 +42 482.0 +43 483.0 +44 487.5 +45 471.5 +46 471.0 +47 468.5 +48 473.5 +49 472.0 +50 437.5 +51 415.5 +52 407.5 +53 398.0 +54 381.0 +55 364.0 +56 349.0 +57 341.0 +58 330.5 +59 320.0 +60 320.5 +61 301.0 +62 275.0 +63 245.0 +64 207.0 +65 190.5 +66 182.0 +67 173.0 +68 167.0 +69 151.5 +70 131.5 +71 121.0 +72 117.5 +73 110.5 +74 104.0 +75 90.5 +76 75.0 +77 67.5 +78 62.5 +79 61.5 +80 59.5 +81 57.0 +82 54.5 +83 47.0 +84 39.5 +85 38.0 +86 36.5 +87 35.5 +88 28.0 +89 21.0 +90 19.5 +91 17.0 +92 15.5 +93 14.5 +94 13.5 +95 13.5 +96 15.0 +97 15.5 +98 17.0 +99 16.0 +100 13.5 +>>END_MODULE +>>Per base N content pass +#Base N-Count +1 0.0 +2 0.0 +3 0.0 +4 0.0 +5 0.0 +6 0.0 +7 0.0 +8 0.0 +9 0.0 +10-11 0.0 +12-13 0.0 +14-15 0.0 +16-17 0.0 +18-19 0.0 +20-21 0.0 +22-23 0.0 +24-25 0.0 +26-27 0.0 +28-29 0.0 +30-31 0.0 +32-33 0.0 +34-35 0.0 +36-37 0.0 +38-39 0.0 +40-41 0.0 +42-43 0.0 +44-45 0.0 +46-47 0.0 +48-49 0.0 +50-51 0.0 +52-53 0.0 +54-55 0.0 +56-57 0.0 +58-59 0.0 +60-61 0.0 +62-63 0.0 +64-65 0.0 +66-67 0.0 +68-69 0.0 +70-71 0.0 +72-73 0.0 +74-75 0.0 +76-77 0.0 +78-79 0.0 +80-81 0.0 +82-83 0.0 +84-85 0.0 +86-87 0.0 +88-89 0.0 +90-91 0.0 +92-93 0.0 +94-95 0.0 +96-97 0.0 +98-99 0.0 +100-101 0.0 +102-103 0.0 +104-105 0.0 +106-107 0.0 +108 0.0 +>>END_MODULE +>>Sequence Length Distribution warn +#Length Count +0-4 98.0 +5-9 250.0 +10-14 257.0 +15-19 279.0 +20-24 321.0 +25-29 396.0 +30-34 401.0 +35-39 433.0 +40-44 401.0 +45-49 385.0 +50-54 320.0 +55-59 5.0 +60-64 1.0 +65-69 0.0 +70-74 0.0 +75-79 0.0 +80-84 0.0 +85-89 0.0 +90-94 0.0 +95-99 33.0 +100-104 0.0 +105-109 1325.0 +>>END_MODULE +>>Sequence Duplication Levels pass +#Total Deduplicated Percentage 98.73598369011212 +#Duplication Level Percentage of deduplicated Percentage of total +1 99.44249432170142 98.1855249745158 +2 0.47491224447656405 0.9378185524974516 +3 0.041296716911005574 0.12232415902140673 +4 0.020648358455502787 0.08154943934760449 +5 0.0 0.0 +6 0.0 0.0 +7 0.0 0.0 +8 0.0 0.0 +9 0.0 0.0 +>10 0.020648358455502787 0.672782874617737 +>50 0.0 0.0 +>100 0.0 0.0 +>500 0.0 0.0 +>1k 0.0 0.0 +>5k 0.0 0.0 +>10k+ 0.0 0.0 +>>END_MODULE +>>Overrepresented sequences warn +#Sequence Count Percentage Possible Source +ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit +>>END_MODULE +>>Adapter Content pass +#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter +1 0.0 0.0 0.0 0.0 0.0 +2 0.0 0.0 0.0 0.0 0.0 +3 0.0 0.0 0.0 0.0 0.0 +4 0.0 0.0 0.0 0.0 0.0 +5 0.0 0.0 0.0 0.0 0.0 +6 0.0 0.0 0.0 0.0 0.0 +7 0.0 0.0 0.0 0.0 0.0 +8 0.0 0.0 0.0 0.0 0.0 +9 0.0 0.0 0.0 0.0 0.0 +10-11 0.0 0.0 0.0 0.0 0.0 +12-13 0.0 0.0 0.0 0.0 0.0 +14-15 0.0 0.0 0.0 0.0 0.0 +16-17 0.0 0.0 0.0 0.0 0.0 +18-19 0.0 0.0 0.0 0.0 0.0 +20-21 0.12232415902140673 0.0 0.0 0.0 0.0 +22-23 0.12232415902140673 0.0 0.0 0.0 0.0 +24-25 0.12232415902140673 0.0 0.0 0.0 0.0 +26-27 0.1325178389398573 0.0 0.0 0.0 0.0 +28-29 0.2038735983690112 0.0 0.0 0.0 0.0 +30-31 0.22426095820591233 0.0 0.0 0.0 0.0 +32-33 0.22426095820591233 0.0 0.0 0.0 0.0 +34-35 0.24464831804281345 0.0 0.0 0.0 0.0 +36-37 0.3058103975535168 0.0 0.0 0.0 0.0 +38-39 0.4383282364933741 0.0 0.0 0.0 0.0 +40-41 0.4689092762487258 0.0 0.0 0.0 0.0 +42-43 0.4689092762487258 0.0 0.0 0.0 0.0 +44-45 0.4689092762487258 0.0 0.0 0.0 0.0 +46-47 0.4689092762487258 0.0 0.0 0.0 0.0 +48-49 0.4689092762487258 0.0 0.0 0.0 0.0 +50-51 0.4689092762487258 0.0 0.0 0.0 0.0 +52-53 0.4689092762487258 0.0 0.0 0.0 0.0 +54-55 0.4689092762487258 0.0 0.0 0.0 0.0 +56-57 0.4689092762487258 0.0 0.0 0.0 0.0 +58-59 0.4689092762487258 0.0 0.0 0.0 0.0 +60-61 0.4689092762487258 0.0 0.0 0.0 0.0 +62-63 0.4689092762487258 0.0 0.0 0.0 0.0 +64-65 0.4689092762487258 0.0 0.0 0.0 0.0 +66-67 0.4689092762487258 0.0 0.0 0.0 0.0 +68-69 0.4689092762487258 0.0 0.0 0.0 0.0 +70-71 0.4689092762487258 0.0 0.0 0.0 0.0 +72-73 0.4689092762487258 0.0 0.0 0.0 0.0 +74-75 0.4689092762487258 0.0 0.0 0.0 0.0 +76-77 0.4689092762487258 0.0 0.0 0.0 0.0 +78-79 0.4689092762487258 0.0 0.0 0.0 0.0 +80-81 0.4689092762487258 0.0 0.0 0.0 0.0 +82-83 0.4689092762487258 0.0 0.0 0.0 0.0 +84-85 0.4689092762487258 0.0 0.0 0.0 0.0 +86-87 0.4689092762487258 0.0 0.0 0.0 0.0 +88-89 0.4689092762487258 0.0 0.0 0.0 0.0 +90-91 0.4689092762487258 0.0 0.0 0.0 0.0 +92-93 0.4689092762487258 0.0 0.0 0.0 0.0 +94-95 0.4689092762487258 0.0 0.0 0.0 0.0 +96-97 0.47910295616717635 0.0 0.0 0.0 0.0 +>>END_MODULE +>>Kmer Content pass +>>END_MODULE