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view test-data/fastqc_data_customlimits.txt @ 18:3e1cdf5406db draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fastqc commit 8a5d4f0470a7778a87d01dc84578f94a30ab7f43
| author | iuc |
|---|---|
| date | Mon, 05 Nov 2018 12:08:29 -0500 |
| parents | |
| children | 3d0c7bdf12f5 |
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##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4905 Sequences flagged as poor quality 0 Sequence length 1-108 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.282772680937818 31.0 27.0 33.0 23.0 34.0 2 28.832925336597306 30.0 27.0 32.0 22.0 33.0 3 28.896953588223266 30.0 27.0 32.0 23.0 34.0 4 29.006785934608267 30.0 27.0 32.0 23.0 34.0 5 28.9377990430622 30.0 27.0 32.0 22.0 34.0 6 29.002938706968934 30.0 27.0 32.0 23.0 34.0 7 28.899830220713074 30.0 27.0 32.0 22.0 34.0 8 28.900706184463942 30.0 27.0 32.0 22.0 33.0 9 28.754602555772145 30.0 27.0 32.0 22.0 34.0 10-11 28.822083022456294 30.0 27.0 32.0 22.5 33.0 12-13 28.74898578710789 30.0 26.5 32.0 22.0 33.0 14-15 28.53370750727077 30.0 26.0 32.0 22.0 33.5 16-17 28.52661127583153 30.0 26.0 32.0 22.0 33.0 18-19 28.68343192244741 30.0 26.0 32.0 22.5 33.0 20-21 28.370130166336985 29.5 26.0 32.0 22.0 33.0 22-23 28.185946266098476 29.0 26.0 32.0 22.0 33.0 24-25 28.13009469533431 29.0 26.0 32.0 21.5 33.0 26-27 28.075768267577 29.0 26.0 32.0 21.5 33.0 28-29 27.828024188665616 29.0 25.0 32.0 21.0 33.0 30-31 27.792826876513317 29.0 25.0 31.5 21.0 33.0 32-33 27.490989073616355 28.0 25.0 31.0 21.0 33.0 34-35 27.357199679261562 28.0 24.5 31.0 21.0 33.0 36-37 27.062158129410527 28.0 24.0 31.0 20.5 33.0 38-39 27.238022216783758 28.0 24.0 31.0 21.0 33.0 40-41 27.028963434226593 28.0 24.0 31.0 20.5 33.0 42-43 26.90862078496675 27.0 24.0 31.0 20.5 33.0 44-45 26.632003369751054 27.0 24.0 30.0 20.0 32.0 46-47 26.83042786373109 27.5 24.0 31.0 20.5 32.0 48-49 26.431681832255798 27.0 23.5 30.0 20.0 32.0 50-51 26.219000735104444 27.0 23.0 30.0 20.0 32.0 52-53 25.925690423864786 26.5 22.5 29.5 19.5 31.5 54-55 27.995241016389798 29.5 25.5 31.5 20.5 33.0 56-57 30.410857897242785 31.5 28.5 33.0 25.5 34.0 58-59 30.4083885209713 31.5 28.5 33.0 25.5 34.0 60-61 30.444812362030905 31.5 29.0 33.0 26.0 34.0 62-63 30.44039735099338 31.5 29.0 33.0 25.5 34.0 64-65 30.441712697003883 31.5 29.0 33.0 25.5 34.0 66-67 30.51141384388807 32.0 29.0 33.0 25.5 34.0 68-69 30.05117820324006 31.0 28.0 33.0 24.5 34.0 70-71 30.276141384388808 31.0 29.0 33.0 25.0 34.0 72-73 30.385493372606774 31.5 29.0 33.0 25.5 34.0 74-75 30.213549337260677 31.0 28.5 33.0 25.5 34.0 76-77 29.678939617083948 31.0 28.0 33.0 24.5 34.0 78-79 30.019145802650957 31.0 28.0 33.0 24.5 34.0 80-81 29.664212076583212 31.0 27.5 33.0 24.0 34.0 82-83 29.653534609720175 31.0 28.0 32.5 24.5 34.0 84-85 29.425625920471283 30.5 27.0 32.0 24.0 34.0 86-87 29.34057437407953 30.0 27.0 32.5 24.0 34.0 88-89 28.785714285714285 30.0 27.0 32.0 22.5 33.5 90-91 28.667525773195877 29.5 26.0 32.0 23.0 33.0 92-93 28.565905743740796 29.0 26.0 32.0 23.0 33.0 94-95 28.271723122238583 29.0 26.0 32.0 22.5 33.0 96-97 27.94955817378498 29.0 25.5 31.0 22.0 33.0 98-99 27.641132075471695 28.0 25.0 31.0 21.5 33.0 100-101 27.614716981132077 28.0 25.0 31.0 21.5 33.0 102-103 27.196226415094337 28.0 24.5 31.0 21.0 32.5 104-105 26.870943396226416 27.5 24.0 31.0 20.0 32.5 106-107 26.367593937711003 27.0 23.5 30.0 20.5 32.0 108 27.5650623885918 28.0 24.0 31.0 22.0 33.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 20 7.0 21 24.0 22 47.0 23 78.0 24 226.0 25 513.0 26 830.0 27 1017.0 28 947.0 29 645.0 30 352.0 31 157.0 32 55.0 33 6.0 34 1.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.223241590214066 33.00713557594291 25.97349643221203 19.79612640163099 2 23.76580987352101 31.415748674010608 27.315381476948186 17.503059975520195 3 21.876916785933346 28.70578613780413 29.46227765283173 19.95501942343079 4 21.55048324079786 28.295290972650626 28.850503804236066 21.303721982315444 5 21.219055543998337 30.205949656750576 28.271271063033076 20.303723736218014 6 22.124265323257767 28.946263643996637 26.364399664147776 22.565071368597817 7 21.943972835314092 29.9660441426146 28.395585738539896 19.69439728353141 8 21.378129681147016 28.825165846351382 27.8621870318853 21.934517440616304 9 20.077972709551656 29.088152479965345 28.654970760233915 22.17890405024908 10-11 21.243237275035884 29.468919068124105 27.680247322512976 21.607596334327038 12-13 20.8276950834461 29.03698691926026 29.18358141632837 20.951736580965267 14-15 20.24001846295869 28.975305792753286 29.471497807523654 21.313177936764365 16-17 19.83872880351002 29.396418830783826 29.621724178821296 21.14312818688486 18-19 20.845552297165202 27.871456500488755 29.973118279569892 21.309872922776147 20-21 20.538172715894866 28.973717146433042 29.111389236545683 21.376720901126408 22-23 20.88082901554404 30.129533678756477 29.300518134715027 19.689119170984455 24-25 20.506974248927037 29.07725321888412 29.05042918454936 21.365343347639485 26-27 20.86603139013453 28.054932735426007 30.339125560538115 20.739910313901344 28-29 19.32600732600733 30.842490842490843 28.776556776556777 21.054945054945055 30-31 20.638820638820636 29.19226044226044 29.49938574938575 20.669533169533167 32-33 19.47989016314004 29.009853012437407 29.38136003876595 22.1288967856566 34-35 19.6674020023757 29.713219073477003 30.205328355676226 20.41405056847107 36-37 20.443482963764197 29.853975121687398 30.61114115738237 19.091400757166035 38-39 20.992661259173424 28.041714947856317 30.088837388953266 20.876786404016993 40-41 19.860197368421055 28.680098684210524 30.32483552631579 21.134868421052634 42-43 20.01321003963012 27.873183619550858 30.823425803610743 21.29018053720828 44-45 18.941371943982908 30.192262046047947 29.954901495371466 20.911464514597675 46-47 20.1888718734048 28.126595201633485 31.470137825421133 20.21439509954058 48-49 19.56159822419534 29.689234184239734 28.77358490566038 21.975582685904552 50-51 20.50656087885261 28.2575526396094 30.759841318278912 20.47604516325908 52-53 17.691256830601095 29.918032786885245 31.864754098360653 20.525956284153004 54-55 17.214912280701753 32.30994152046784 29.53216374269006 20.942982456140353 56-57 19.30856932695844 30.893710923133504 30.893710923133504 18.90400882677455 58-59 20.014716703458426 29.72774098601913 31.42016188373804 18.8373804267844 60-61 17.402501839587934 33.18616629874908 29.506990434142754 19.904341427520237 62-63 18.543046357615893 29.72774098601913 32.2663723325975 19.462840323767477 64-65 16.893632683106365 32.94074346705926 30.180345969819655 19.985277880014724 66-67 18.519882179675996 28.902798232695137 32.80559646539028 19.771723122238587 68-69 17.2680412371134 30.375552282768776 31.66421207658321 20.692194403534607 70-71 17.673048600883654 27.798232695139912 35.34609720176731 19.182621502209134 72-73 16.200294550810014 29.528718703976438 33.87334315169367 20.39764359351988 74-75 18.372606774668633 28.019145802650957 34.5360824742268 19.072164948453608 76-77 16.678939617083945 31.70103092783505 31.73784977908689 19.88217967599411 78-79 18.77761413843888 28.387334315169365 32.62150220913107 20.213549337260677 80-81 16.384388807069218 30.927835051546392 32.9160530191458 19.771723122238587 82-83 17.083946980854197 30.927835051546392 32.54786450662739 19.44035346097202 84-85 16.936671575846834 31.590574374079527 31.443298969072163 20.029455081001473 86-87 17.488954344624446 27.908689248895435 34.20471281296024 20.39764359351988 88-89 16.789396170839467 32.36377025036819 31.075110456553755 19.771723122238587 90-91 19.587628865979383 29.749631811487482 33.5419734904271 17.120765832106038 92-93 17.746686303387335 30.04418262150221 31.33284241531664 20.876288659793815 94-95 18.924889543446245 28.460972017673047 33.39469808541973 19.219440353460975 96-97 16.42120765832106 29.418262150220915 32.47422680412371 21.686303387334316 98-99 18.158090976882924 29.34377330350485 33.1469052945563 19.351230425055927 100-101 17.77358490566038 29.849056603773583 33.28301886792453 19.09433962264151 102-103 18.339622641509436 29.509433962264154 30.90566037735849 21.245283018867926 104-105 18.037735849056606 28.67924528301887 31.77358490566038 21.50943396226415 106-107 17.2782874617737 29.357798165137616 33.52446483180428 19.839449541284402 108 20.053475935828878 26.38146167557932 31.72905525846702 21.836007130124777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 15.5 2 16.5 3 17.0 4 18.0 5 21.5 6 26.5 7 30.0 8 33.5 9 36.0 10 41.0 11 47.0 12 47.5 13 56.0 14 65.5 15 69.0 16 72.5 17 78.0 18 86.5 19 94.5 20 105.5 21 113.0 22 123.0 23 132.5 24 149.5 25 172.5 26 200.0 27 222.0 28 247.0 29 282.0 30 310.5 31 337.0 32 370.0 33 403.5 34 438.0 35 465.0 36 481.5 37 511.0 38 523.5 39 506.0 40 491.0 41 491.5 42 482.0 43 483.0 44 487.5 45 471.5 46 471.0 47 468.5 48 473.5 49 472.0 50 437.5 51 415.5 52 407.5 53 398.0 54 381.0 55 364.0 56 349.0 57 341.0 58 330.5 59 320.0 60 320.5 61 301.0 62 275.0 63 245.0 64 207.0 65 190.5 66 182.0 67 173.0 68 167.0 69 151.5 70 131.5 71 121.0 72 117.5 73 110.5 74 104.0 75 90.5 76 75.0 77 67.5 78 62.5 79 61.5 80 59.5 81 57.0 82 54.5 83 47.0 84 39.5 85 38.0 86 36.5 87 35.5 88 28.0 89 21.0 90 19.5 91 17.0 92 15.5 93 14.5 94 13.5 95 13.5 96 15.0 97 15.5 98 17.0 99 16.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-4 98.0 5-9 250.0 10-14 257.0 15-19 279.0 20-24 321.0 25-29 396.0 30-34 401.0 35-39 433.0 40-44 401.0 45-49 385.0 50-54 320.0 55-59 5.0 60-64 1.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 33.0 100-104 0.0 105-109 1325.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.73598369011212 #Duplication Level Percentage of deduplicated Percentage of total 1 99.44249432170142 98.1855249745158 2 0.47491224447656405 0.9378185524974516 3 0.041296716911005574 0.12232415902140673 4 0.020648358455502787 0.08154943934760449 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.020648358455502787 0.672782874617737 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672782874617737 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.12232415902140673 0.0 0.0 0.0 0.0 22-23 0.12232415902140673 0.0 0.0 0.0 0.0 24-25 0.12232415902140673 0.0 0.0 0.0 0.0 26-27 0.1325178389398573 0.0 0.0 0.0 0.0 28-29 0.2038735983690112 0.0 0.0 0.0 0.0 30-31 0.22426095820591233 0.0 0.0 0.0 0.0 32-33 0.22426095820591233 0.0 0.0 0.0 0.0 34-35 0.24464831804281345 0.0 0.0 0.0 0.0 36-37 0.3058103975535168 0.0 0.0 0.0 0.0 38-39 0.4383282364933741 0.0 0.0 0.0 0.0 40-41 0.4689092762487258 0.0 0.0 0.0 0.0 42-43 0.4689092762487258 0.0 0.0 0.0 0.0 44-45 0.4689092762487258 0.0 0.0 0.0 0.0 46-47 0.4689092762487258 0.0 0.0 0.0 0.0 48-49 0.4689092762487258 0.0 0.0 0.0 0.0 50-51 0.4689092762487258 0.0 0.0 0.0 0.0 52-53 0.4689092762487258 0.0 0.0 0.0 0.0 54-55 0.4689092762487258 0.0 0.0 0.0 0.0 56-57 0.4689092762487258 0.0 0.0 0.0 0.0 58-59 0.4689092762487258 0.0 0.0 0.0 0.0 60-61 0.4689092762487258 0.0 0.0 0.0 0.0 62-63 0.4689092762487258 0.0 0.0 0.0 0.0 64-65 0.4689092762487258 0.0 0.0 0.0 0.0 66-67 0.4689092762487258 0.0 0.0 0.0 0.0 68-69 0.4689092762487258 0.0 0.0 0.0 0.0 70-71 0.4689092762487258 0.0 0.0 0.0 0.0 72-73 0.4689092762487258 0.0 0.0 0.0 0.0 74-75 0.4689092762487258 0.0 0.0 0.0 0.0 76-77 0.4689092762487258 0.0 0.0 0.0 0.0 78-79 0.4689092762487258 0.0 0.0 0.0 0.0 80-81 0.4689092762487258 0.0 0.0 0.0 0.0 82-83 0.4689092762487258 0.0 0.0 0.0 0.0 84-85 0.4689092762487258 0.0 0.0 0.0 0.0 86-87 0.4689092762487258 0.0 0.0 0.0 0.0 88-89 0.4689092762487258 0.0 0.0 0.0 0.0 90-91 0.4689092762487258 0.0 0.0 0.0 0.0 92-93 0.4689092762487258 0.0 0.0 0.0 0.0 94-95 0.4689092762487258 0.0 0.0 0.0 0.0 96-97 0.47910295616717635 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE