Mercurial > repos > devteam > fastx_nucleotides_distribution
comparison fastx_nucleotides_distribution.xml @ 2:9353010b95df draft
Uploaded tool help images.
author | devteam |
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date | Tue, 03 Jun 2014 15:09:10 -0400 |
parents | 63d6e2daad48 |
children | 21796f46e0ba |
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1:63d6e2daad48 | 2:9353010b95df |
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27 **Output Examples** | 27 **Output Examples** |
28 | 28 |
29 The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT** | 29 The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT** |
30 | 30 |
31 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_1.png | 31 .. image:: fastq_nucleotides_distribution_1.png |
32 | 32 |
33 In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...** | 33 In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...** |
34 | 34 |
35 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_2.png | 35 .. image:: fastq_nucleotides_distribution_2.png |
36 | 36 |
37 The following chart shows a growing number of unknown (N) nucleotides towards later cycles (which might indicate a sequencing problem): | 37 The following chart shows a growing number of unknown (N) nucleotides towards later cycles (which might indicate a sequencing problem): |
38 | 38 |
39 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_3.png | 39 .. image:: fastq_nucleotides_distribution_3.png |
40 | 40 |
41 But most of the time, the chart will look rather random: | 41 But most of the time, the chart will look rather random: |
42 | 42 |
43 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_4.png | 43 .. image:: fastq_nucleotides_distribution_4.png |
44 | 44 |
45 ------ | 45 ------ |
46 | 46 |
47 This tool is based on `FASTX-toolkit`__ by Assaf Gordon. | 47 This tool is based on `FASTX-toolkit`__ by Assaf Gordon. |
48 | 48 |