comparison fastx_nucleotides_distribution.xml @ 2:9353010b95df draft

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author devteam
date Tue, 03 Jun 2014 15:09:10 -0400
parents 63d6e2daad48
children 21796f46e0ba
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1:63d6e2daad48 2:9353010b95df
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27 **Output Examples** 27 **Output Examples**
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29 The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT** 29 The following chart clearly shows the barcode used at the 5'-end of the library: **GATCT**
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31 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_1.png 31 .. image:: fastq_nucleotides_distribution_1.png
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33 In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...** 33 In the following chart, one can almost 'read' the most abundant sequence by looking at the dominant values: **TGATA TCGTA TTGAT GACTG AA...**
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35 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_2.png 35 .. image:: fastq_nucleotides_distribution_2.png
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37 The following chart shows a growing number of unknown (N) nucleotides towards later cycles (which might indicate a sequencing problem): 37 The following chart shows a growing number of unknown (N) nucleotides towards later cycles (which might indicate a sequencing problem):
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39 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_3.png 39 .. image:: fastq_nucleotides_distribution_3.png
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41 But most of the time, the chart will look rather random: 41 But most of the time, the chart will look rather random:
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43 .. image:: ${static_path}/fastx_icons/fastq_nucleotides_distribution_4.png 43 .. image:: fastq_nucleotides_distribution_4.png
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45 ------ 45 ------
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47 This tool is based on `FASTX-toolkit`__ by Assaf Gordon. 47 This tool is based on `FASTX-toolkit`__ by Assaf Gordon.
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