view freebayes.xml @ 0:33a0e6aca936 draft default tip

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<?xml version="1.0"?>
<tool id="freebayes_wrapper" name="Call SNPS with Freebayes" version="0.5.0">
  <requirements>
    <requirement type="package" version="0.9.6_9608597d12e127c847ae03aa03440ab63992fedf">freebayes</requirement>
    <requirement type="package" version="0.1.16">samtools</requirement>
  </requirements>
  <description>Bayesian genetic variant detector</description>
  <command>
    ln -s $reference localref.fa &amp;&amp;
    ln -s $bamfile localbam.bam &amp;&amp;
    samtools faidx localref.fa 2&gt;&amp;1 || echo "Error running samtools faidx for FreeBayes" &gt;&amp;2 &amp;&amp;
    samtools sort localbam.bam localbam.bam 2&gt;&amp;1 || echo "Error running samtools sort for FreeBayes" &gt;&amp;2 &amp;&amp;
    samtools index localbam.bam 2&gt;&amp;1 || echo "Error running samtools index for FreeBayes" &gt;&amp;2 &amp;&amp;
    freebayes --fasta-reference localref.fa localbam.bam --vcf $output
            #if $params.source_select == "full":
                $params.showRefRepeats
                -T $params.theta
                -p $params.ploidy
                $params.pooled
                $params.mnps
                $params.nosnps
                -n $params.bestAlleles
                $params.allAlleles
                $params.duplicateReads
                -M $params.refMapQuality
                $params.ignoreRefAllele
                $params.haploidReference
                -m $params.minMapQuality
                -q $params.minBaseQuality
                $params.noFilters
                -x $params.indelExclusionWindow
                <!-- -D $readDependenceFactor -->
                -V $params.diffusionPriorScalar
                -W $params.postIntegBandwidth
                -Y $params.postIntegBanddepth
                -F $params.minAltFraction
                -C $params.minAltCount
                -G $params.minAltTotal
                --min-coverage $params.minCoverage
            #end if
  </command>
  <inputs>
    <param format="fasta" name="reference" type="data" metadata_name="dbkey" label="Reference File"/>
    <param format="bam" name="bamfile" type="data" label="Bam Alignment File"/>
    <conditional name="params">
      <param name="source_select" type="select" label="Freebayes Settings to Use" help="For straight forward mapping needs use Commonly Used settings. If you want full control use Full Parameter List">
        <option value="pre_set">Commonly Used</option>
        <option value="full">Full Parameter List</option>
      </param>
      <when value="pre_set"/>
      <when value="full">
        <param name="indels" type="select" label="Include insertion and deletion alleles in the analysis">
          <option value="">No</option>
          <option value="-i -N --report-all-alternates --left-align-indels">Yes</option>
        </param>
        <param name="theta" size="5" type="float" value="0.001" label="Theta" help="The expected mutation rate or pairwise nucleotide diversity among the population under analysis.  This serves as the single parameter to the Ewens Sampling Formula prior model"/>
        <param name="showRefRepeats" type="select" label="Show Reference Repeats" help="Calculate and show information about reference repeats in the VCF output">
          <option value="">No</option>
          <option value="-_">Yes</option>
        </param>
        <param name="ploidy" size="5" type="integer" value="2" label="Ploidy" help="Sets the default ploidy for the analysis"/>
        <param name="pooled" type="select" label="Pooled" help="Assume that samples result from pooled sequencing. When using this flag, set --ploidy to the number of alleles in each sample">
          <option value="">No</option>
          <option value="-J">Yes</option>
        </param>
        <param name="mnps" type="select" label="Include multi-nuceotide polymorphisms in the analysis">
          <option value="">No</option>
          <option value="--mnps">Yes</option>
        </param>
        <param name="nosnps" type="select" label="Ignore SNP alleles">
          <option value="">No</option>
          <option value="--no-snps">Yes</option>
        </param>
        <param name="duplicateReads" type="select" label="Include duplicate-marked alignments in the analysis">
          <option value="">No</option>
          <option value="--use-duplicate-reads">Yes</option>
        </param>
        <param name="bestAlleles" size="5" type="integer" value="2" label="Use Best N Alleles" help="Evaluate only the best N alleles, ranked by sum of supporting quality scores"/>
        <param name="allAlleles" type="select" label="Evaluate all possible alleles">
          <option value="">No</option>
          <option value="--use-all-alleles">Yes</option>
        </param>
        <param name="refMapQuality" size="5" type="integer" value="100" label="Assign mapping quality of Q to the reference allele at each site"/>
        <param name="refBaseQuality" size="5" type="integer" value="60" label="Reference Base Quality" help="Assign a base quality of Q to the reference allele at each site"/>
        <param name="minMapQuality" size="5" type="integer" value="10" label="Minimum Mapping Quality" help="Exclude alignments from analysis if they have a mapping quality less than Q"/>
        <param name="minBaseQuality" size="5" type="integer" value="5" label="Minimum Base Quality" help="Exclude alleles from analysis if their supporting base quality is less than Q"/>
        <param name="indelExclusionWindow" size="5" type="integer" value="0" label="Indel Exclusion Window" help="Ignore portions of alignments N bases from a putative insertion or deletion allele"/>
        <param name="ignoreRefAllele" type="select" label="Ignore Reference Allele" help="By default, the reference allele is considered as another sample.  This flag excludes it from the analysis">
          <option value="">No</option>
          <option value="--ignore-reference-allele">Yes</option>
        </param>
        <param name="haploidReference" type="select" label="Haploid Reference" help="If using the reference sequence as a sample, consider it to be haploid">
          <option value="">No</option>
          <option value="--haploid-reference">Yes</option>
        </param>
        <param name="noFilters" type="select" label="No Filters" help="Do not use any input base and mapping quality filters. Equivalent to -m 0 -q 0 -R 0 -S 0">
          <option value="">No</option>
          <option value="--no-filters">Yes</option>
        </param>
        <!-- <param name="readDependenceFactor" size="5" type="float" value="0.9" label="Read Dependence Factor" help="Incorporate non-independence of reads by scaling successive observations by this factor during data likelihood calculations"/> -->
        <param name="diffusionPriorScalar" size="5" type="float" value="1" label="Diffusion Prior Scalar" help="Downgrade the significance of P(genotype combo | allele frequency) by taking the Nth root of this component of the prior"/>
        <param name="postIntegBandwidth" size="5" type="integer" value="2" label="Posterior Integratoin Bandwidth" help="Integrate all genotype combinations in our posterior space which lie no more than N steps from the most likely combination in terms of data likelihoods, taking the N steps from the most to least likely genotype for each individual"/>
        <param name="postIntegBanddepth" size="5" type="integer" value="2" label="Posterior Integratoin Banddepth" help="Generate all genotype combinations for which up to this number of samples have up to their -W'th worst genotype according to data likelihood"/>
        <param name="minAltFraction" size="5" type="integer" value="0" label="Minimum Alternative Fraction" help="Require at least this fraction of observations supporting an alternate allele within a single individual in the in order to evaluate the position"/>
        <param name="minAltCount" size="5" type="integer" value="1" label="Minimum Alternative Count" help="Require at least this count of observations supporting an alternate allele within a single individual in order to evaluate the position"/>
        <param name="minAltTotal" size="5" type="integer" value="1" label="Minimum Alternative Total" help="Require at least this count of observations supporting an alternate allele within the total population in order to use the allele in analysis"/>
        <param name="minCoverage" size="5" type="integer" value="0" label="Minimum Coverage" help="Require at least this coverage to process a site"/>
      </when>
    </conditional>
  </inputs>
  <outputs>
    <data format="vcf" name="output" metadata_source="reference" />
  </outputs>
  <tests>
    <test>
      <param name="reference" ftype="fasta" value="mosaik_test_ref.fasta"/>
      <param name="bamfile" ftype="bam" value="freebayes_in.bam"/>
      <param name="source_select" value="pre_set"/>
      <output name="output" file="freebayes_out.vcf" lines_diff="4"/>
    </test>
  </tests>
  <help>
This tool uses Freebayes to call SNPS given a reference sequence and a BAM alignment file.
  </help>
</tool>