# HG changeset patch # User devteam # Date 1465336695 14400 # Node ID 12aeae6d858713ba7cdc537b32d57efc6a930f2e # Parent 48fe74f391abd6c94446ec340d869f99c5256c2d planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tool_collections/cufflinks/gffread commit 3bc5271145f939d85bb709fc95197be66b348328 diff -r 48fe74f391ab -r 12aeae6d8587 cuff_macros.xml --- a/cuff_macros.xml Wed Nov 11 12:36:04 2015 -0500 +++ b/cuff_macros.xml Tue Jun 07 17:58:15 2016 -0400 @@ -1,11 +1,13 @@ 2.2.1 + cufflinks + diff -r 48fe74f391ab -r 12aeae6d8587 gffread.xml --- a/gffread.xml Wed Nov 11 12:36:04 2015 -0500 +++ b/gffread.xml Tue Jun 07 17:58:15 2016 -0400 @@ -18,7 +18,7 @@ - @@ -54,7 +54,7 @@ #if $reference_genome.source == 'history': ln -s $reference_genome.genome_fasta genomeref.fa && #end if - gffread $input + gffread $input #if $reference_genome.source == 'cached': -g "${reference_genome.fasta_indexes.fields.path}" #if $reference_genome.ref_filtering and str($reference_genome.ref_filtering) != '': @@ -121,7 +121,7 @@ - + @@ -131,21 +131,21 @@ - examples:
- 1000..500000
- chr1:1000..500000
- +chr1:1000..500000
+ (-r [['strand']'chr':]'start'..'end')
+ examples:
+ 1000..500000
+ chr1:1000..500000
+ +chr1:1000..500000
-chr1:1000..500000 ]]>
(([+-])?(\w+:))?\d+\.\.\d+ -
- @@ -154,7 +154,7 @@ NOTE: GFF records on reference sequences that are not found among the "original_ref_ID" entries in this file will be filtered out ]]> - + @@ -201,7 +201,7 @@ - + @@ -209,7 +209,7 @@ - + @@ -227,11 +227,11 @@ - - - @@ -359,11 +359,11 @@ Usage: :: - gffread "input_gff" [-g "genomic_seqs_fasta" | "dir"][-s "seq_info.fsize"] + gffread "input_gff" [-g "genomic_seqs_fasta" | "dir"][-s "seq_info.fsize"] [-o "outfile.gff"] [-t "tname"] [-r [["strand"]"chr":]"start".."end" [-R]] [-CTVNJMKQAFGUBHZWTOLE] [-w "exons.fa"] [-x "cds.fa"] [-y "tr_cds.fa"] - [-i "maxintron"] - + [-i "maxintron"] + Options: :: -g full path to a multi-fasta file with the genomic sequences @@ -376,7 +376,7 @@ -i discard transcripts having an intron larger than -r only show transcripts overlapping coordinate range .. (on chromosome/contig , strand if provided) - -R for -r option, discard all transcripts that are not fully + -R for -r option, discard all transcripts that are not fully contained within the given range -U discard single-exon transcripts -C coding only: discard mRNAs that have no CDS feature @@ -385,12 +385,12 @@ and move them to the mRNA level (useful for GTF input) -A use the description field from and add it as the value for a 'descr' attribute to the GFF record - + -O process also non-transcript GFF records (by default non-transcript records are ignored) -V discard any mRNAs with CDS having in-frame stop codons -H for -V option, check and adjust the starting CDS phase - if the original phase leads to a translation with an + if the original phase leads to a translation with an in-frame stop codon -B for -V option, single-exon transcripts are also checked on the opposite strand @@ -400,7 +400,7 @@ or the terminal STOP codon, or have an in-frame stop codon (only print mRNAs with a fulll, valid CDS) --no-pseudo: filter out records matching the 'pseudo' keyword - + -M/--merge : cluster the input transcripts into loci, collapsing matching transcripts (those with the same exact introns and fully contained) -d : for -M option, write collapsing info to file @@ -410,10 +410,10 @@ -Q for -M option, remove the containment restriction: (multi-exon transcripts will be collapsed if just their introns match, while single-exon transcripts can partially overlap (80%)) - - --force-exons: make sure that the lowest level GFF features are printed as + + --force-exons: make sure that the lowest level GFF features are printed as "exon" features - -E expose (warn about) duplicate transcript IDs and other potential + -E expose (warn about) duplicate transcript IDs and other potential problems with the given GFF/GTF records -D decode url encoded characters within attributes -Z merge close exons into a single exon (for intron size<4)