annotate gi2taxonomy.xml @ 0:7b1b03c4465d draft default tip

Imported from capsule None

<tool id="Fetch Taxonomic Ranks" name="Fetch taxonomic representation" version="1.1.0">
  <description></description>
    <requirements>
        <requirement type="package" version="1.0.0">taxonomy</requirement>
    </requirements>
  <command interpreter="python">gi2taxonomy.py $input $giField $idField $out_file1 ${GALAXY_DATA_INDEX_DIR}</command>
  <inputs>
    <param format="tabular" name="input" type="data" label="Show taxonomic representation for"></param>
    <param name="giField" label="GIs column" type="data_column" data_ref="input" numerical="True" help="select column containing GI numbers"/>
    <param name="idField" label="Name column" type="data_column" data_ref="input" help="select column containing identifiers you want to include into output"/>
  </inputs>
  <outputs>
    <data format="taxonomy" name="out_file1" />
  </outputs>
  <requirements>
    <requirement type="binary">taxBuilder</requirement>
  </requirements>
  <tests>
    <test>
      <param name="input" ftype="tabular" value="taxonomy2gi-input.tabular"/>
      <param name="giField" value="1"/>
      <param name="idField" value="2"/>
      <output name="out_file1" file="taxonomy2gi-output.tabular"/>
    </test>
  </tests>

  <help>

.. class:: infomark

Use *Filter and Sort->Filter* to restrict output of this tool to desired taxonomic ranks. You can also use *Text Manipulation->Cut* to remove unwanted columns from the output.

------

**What it does**

Fetches taxonomic information for a list of GI numbers (sequences identifiers used by the National Center for Biotechnology Information http://www.ncbi.nlm.nih.gov).

-------

**Example**

Suppose you have BLAST output that looks like this::
  
   +-----------------------+----------+----------+-----------------+------------+------+--------+
   | queryId               | targetGI | identity | alignmentLength | mismatches | gaps | score  |
   +-----------------------+----------+----------+-----------------+------------+------+--------+
   | 1L_EYKX4VC01BXWX1_265 |  1430919 |    90.09 |             212 |         15 |    6 | 252.00 | 
   +-----------------------+----------+----------+-----------------+------------+------+--------+

and you want to obtain full taxonomic representation for GIs listed in *targetGI* column. If you set parameters as shown here:

.. image:: ${static_path}/images/fetchTax.png


the tool will generate the following output (you may need to scroll sideways to see the entire line)::

  1                     2    3    4         5       6 7 8        9        10            11       12 13               14       15         16          17        18  19  20 21  22  23           24 25
  1L_EYKX4VC01BXWX1_265 9606 root Eukaryota Metazoa n n Chordata Craniata Gnathostomata Mammalia n  Euarchontoglires Primates Haplorrhini Hominoidea Hominidae n   n   n  Homo n  Homo sapiens n  1430919

In other words the tool printed *Name column*, *taxonomy Id*, appended 22 columns containing taxonomic ranks from Superkingdom to Subspecies and added *GI* as the last column. Below is a formal definition of the output columns::

    Column Definition
   ------- ------------------------------------------
         1 Name (specified by 'Name column' dropdown)
         2 GI   (specified by 'GI column' dropdown)
         3 root
         4 superkingdom
         5 kingdom
         6 subkingdom
         7 superphylum
         8 phylum
         9 subphylum
        10 superclass
        11 class
        12 subclass
        13 superorder
        14 order
        15 suborder
        16 superfamily
        17 family
        18 subfamily
        19 tribe
        20 subtribe
        21 genus
        22 subgenus
        23 species
        24 subspecies

------

.. class:: warningmark

**Why do I have these "n" things?** 

Be aware that the NCBI taxonomy (ftp://ftp.ncbi.nih.gov/pub/taxonomy/) this tool relies upon is incomplete.  This means that for many species one or more ranks are absent and represented as "**n**". In the above example *subkingdom*, *superphylum* etc. are missing.


</help>
</tool>