Mercurial > repos > devteam > ncbi_blast_plus
annotate tools/ncbi_blast_plus/ncbi_macros.xml @ 22:6f386c5dc4fb draft
v0.2.01 add -max_hsps, -use_sw_tback; lists args; internal updates
author | peterjc |
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date | Mon, 18 Sep 2017 06:21:27 -0400 |
parents | 7538e2bfcd41 |
children | 31e517610e1f |
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11
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1 <macros> |
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2 <token name="@WRAPPER_VERSION@">0.2.01</token> |
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v0.1.05 - Update citation information now GigaScience paper is out
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3 <xml name="parallelism"> |
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4 <!-- If job splitting is enabled, break up the query file into parts --> |
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5 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" merge_outputs="output1" /> |
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6 </xml> |
15
c16c30e9ad5b
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7 <xml name="preamble"> |
c16c30e9ad5b
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8 <requirements> |
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v0.2.00, for NCBI BLAST+ 2.5.0 via bioconda or tool_dependencies.xml
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9 <requirement type="package" version="2.5.0">blast</requirement> |
15
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10 </requirements> |
c16c30e9ad5b
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11 <version_command>@BINARY@ -version</version_command> |
c16c30e9ad5b
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12 </xml> |
11
4c4a0da938ff
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13 <xml name="output_change_format"> |
4c4a0da938ff
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14 <change_format> |
13
623f727cdff1
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15 <when input="output.out_format" value="0" format="txt"/> |
623f727cdff1
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16 <when input="output.out_format" value="0 -html" format="html"/> |
623f727cdff1
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peterjc
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17 <when input="output.out_format" value="2" format="txt"/> |
623f727cdff1
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18 <when input="output.out_format" value="2 -html" format="html"/> |
623f727cdff1
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peterjc
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19 <when input="output.out_format" value="4" format="txt"/> |
623f727cdff1
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peterjc
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20 <when input="output.out_format" value="4 -html" format="html"/> |
623f727cdff1
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peterjc
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21 <when input="output.out_format" value="5" format="blastxml"/> |
11
4c4a0da938ff
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peterjc
parents:
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22 </change_format> |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
peterjc
parents:
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23 </xml> |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
peterjc
parents:
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24 <xml name="input_out_format"> |
13
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25 <conditional name="output"> |
22
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26 <param name="out_format" argument="-outfmt" type="select" label="Output format"> |
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27 <option value="6">Tabular (standard 12 columns)</option> |
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28 <option value="ext" selected="true">Tabular (extended 25 columns)</option> |
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29 <option value="cols">Tabular (select which columns)</option> |
623f727cdff1
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30 <option value="5">BLAST XML</option> |
623f727cdff1
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31 <option value="0">Pairwise text</option> |
623f727cdff1
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32 <option value="0 -html">Pairwise HTML</option> |
623f727cdff1
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33 <option value="2">Query-anchored text</option> |
623f727cdff1
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34 <option value="2 -html">Query-anchored HTML</option> |
623f727cdff1
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peterjc
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35 <option value="4">Flat query-anchored text</option> |
623f727cdff1
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peterjc
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36 <option value="4 -html">Flat query-anchored HTML</option> |
623f727cdff1
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peterjc
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37 <!-- |
623f727cdff1
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38 <option value="-outfmt 11">BLAST archive format (ASN.1)</option> |
623f727cdff1
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39 --> |
623f727cdff1
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40 </param> |
623f727cdff1
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41 <when value="6"/> |
623f727cdff1
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42 <when value="ext"/> |
623f727cdff1
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43 <when value="cols"> |
623f727cdff1
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peterjc
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44 <param name="std_cols" type="select" multiple="true" display="checkboxes" label="Standard columns"> |
623f727cdff1
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peterjc
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45 <option selected="true" value="qseqid">qseqid = Query Seq-id (ID of your sequence)</option> |
623f727cdff1
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peterjc
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46 <option selected="true" value="sseqid">sseqid = Subject Seq-id (ID of the database hit)</option> |
623f727cdff1
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peterjc
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47 <option selected="true" value="pident">pident = Percentage of identical matches</option> |
623f727cdff1
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peterjc
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48 <option selected="true" value="length">length = Alignment length</option> |
623f727cdff1
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49 <option selected="true" value="mismatch">mismatch = Number of mismatches</option> |
623f727cdff1
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peterjc
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50 <option selected="true" value="gapopen">gapopen = Number of gap openings</option> |
623f727cdff1
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51 <option selected="true" value="qstart">qstart = Start of alignment in query</option> |
623f727cdff1
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peterjc
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52 <option selected="true" value="qend">qend = End of alignment in query</option> |
623f727cdff1
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peterjc
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53 <option selected="true" value="sstart">sstart = Start of alignment in subject (database hit)</option> |
623f727cdff1
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54 <option selected="true" value="send">send = End of alignment in subject (database hit)</option> |
623f727cdff1
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55 <option selected="true" value="evalue">evalue = Expectation value (E-value)</option> |
623f727cdff1
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peterjc
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56 <option selected="true" value="bitscore">bitscore = Bit score</option> |
623f727cdff1
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57 </param> |
623f727cdff1
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peterjc
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58 <param name="ext_cols" type="select" multiple="true" display="checkboxes" label="Extended columns"> |
623f727cdff1
Uploaded v0.1.00, uses BLAST+ 2.2.29, allows custom column selection for tabular output - including taxonomy fields.
peterjc
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59 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option> |
623f727cdff1
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peterjc
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60 <option value="score">score = Raw score</option> |
623f727cdff1
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61 <option value="nident">nident = Number of identical matches</option> |
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62 <option value="positive">positive = Number of positive-scoring matches</option> |
623f727cdff1
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peterjc
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63 <option value="gaps">gaps = Total number of gaps</option> |
623f727cdff1
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64 <option value="ppos">ppos = Percentage of positive-scoring matches</option> |
623f727cdff1
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peterjc
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65 <option value="qframe">qframe = Query frame</option> |
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66 <option value="sframe">sframe = Subject frame</option> |
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67 <option value="qseq">qseq = Aligned part of query sequence</option> |
623f727cdff1
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68 <option value="sseq">sseq = Aligned part of subject sequence</option> |
623f727cdff1
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peterjc
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69 <option value="qlen">qlen = Query sequence length</option> |
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70 <option value="slen">slen = Subject sequence length</option> |
623f727cdff1
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71 <option value="salltitles">salltitles = All subject title(s), separated by a '<>'</option> |
623f727cdff1
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72 </param> |
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73 <param name="ids_cols" type="select" multiple="true" display="checkboxes" label="Other identifier columns"> |
13
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74 <option value="qgi">qgi = Query GI</option> |
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75 <option value="qacc">qacc = Query accesion</option> |
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76 <option value="qaccver">qaccver = Query accesion.version</option> |
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77 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option> |
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78 <option value="sgi">sgi = Subject GI</option> |
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79 <option value="sallgi">sallgi = All subject GIs</option> |
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80 <option value="sacc">sacc = Subject accession</option> |
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81 <option value="saccver">saccver = Subject accession.version</option> |
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82 <option value="sallacc">sallacc = All subject accessions</option> |
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83 <option value="stitle">stitle = Subject Title</option> |
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84 </param> |
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85 <param name="misc_cols" type="select" multiple="true" display="checkboxes" label="Miscellaneous columns"> |
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86 <option value="sstrand">sstrand = Subject Strand</option> |
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87 <!-- Is it really worth including 'frames' given have 'qframe' and 'sframe'? --> |
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88 <option value="frames">frames = Query and subject frames separated by a '/'</option> |
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89 <option value="btop">btop = Blast traceback operations (BTOP)</option> |
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90 <option value="qcovs">qcovs = Query Coverage Per Subject</option> |
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91 <option value="qcovhsp">qcovhsp = Query Coverage Per HSP</option> |
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92 </param> |
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93 <param name="tax_cols" type="select" multiple="true" display="checkboxes" label="Taxonomy columns"> |
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94 <option value="staxids">staxids = unique Subject Taxonomy ID(s), separated by a ';' (in numerical order)</option> |
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95 <!-- TODO, how to handle the taxonomy data file dependency? If missing these give N/A --> |
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96 <option value="sscinames">sscinames = unique Subject Scientific Name(s), separated by a ';'</option> |
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97 <option value="scomnames">scomnames = unique Subject Common Name(s), separated by a ';'</option> |
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98 <option value="sblastnames">sblastnames = unique Subject Blast Name(s), separated by a ';' (in alphabetical order)</option> |
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99 <option value="sskingdoms">sskingdoms = unique Subject Super Kingdom(s), separated by a ';' (in alphabetical order)</option> |
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100 </param> |
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101 </when> |
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102 <when value="5"/> |
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103 <when value="0"/> |
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104 <when value="0 -html"/> |
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105 <when value="2"/> |
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106 <when value="2 -html"/> |
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107 <when value="4"/> |
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108 <when value="4 -html"/> |
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109 </conditional> |
11
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110 </xml> |
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111 <xml name="input_scoring_matrix"> |
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112 <param argument="-matrix" type="select" label="Scoring matrix"> |
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113 <option value="BLOSUM90">BLOSUM90</option> |
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114 <option value="BLOSUM80">BLOSUM80</option> |
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115 <option value="BLOSUM62" selected="true">BLOSUM62 (default)</option> |
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116 <option value="BLOSUM50">BLOSUM50</option> |
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117 <option value="BLOSUM45">BLOSUM45</option> |
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118 <option value="PAM250">PAM250</option> |
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119 <option value="PAM70">PAM70</option> |
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120 <option value="PAM30">PAM30</option> |
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121 </param> |
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122 </xml> |
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123 <xml name="input_query_gencode"> |
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124 <param argument="-query_gencode" type="select" label="Query genetic code"> |
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125 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details --> |
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126 <option value="1" selected="true">1. Standard</option> |
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127 <option value="2">2. Vertebrate Mitochondrial</option> |
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128 <option value="3">3. Yeast Mitochondrial</option> |
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129 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option> |
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130 <option value="5">5. Invertebrate Mitochondrial</option> |
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131 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option> |
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132 <option value="9">9. Echinoderm Mitochondrial</option> |
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133 <option value="10">10. Euplotid Nuclear</option> |
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134 <option value="11">11. Bacteria and Archaea</option> |
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135 <option value="12">12. Alternative Yeast Nuclear</option> |
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136 <option value="13">13. Ascidian Mitochondrial</option> |
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137 <option value="14">14. Flatworm Mitochondrial</option> |
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138 <option value="15">15. Blepharisma Macronuclear</option> |
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139 <option value="16">16. Chlorophycean Mitochondrial Code</option> |
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140 <option value="21">21. Trematode Mitochondrial Code</option> |
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141 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option> |
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142 <option value="23">23. Thraustochytrium Mitochondrial Code</option> |
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143 <option value="24">24. Pterobranchia mitochondrial code</option> |
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144 </param> |
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145 </xml> |
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146 <xml name="input_db_gencode"> |
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147 <param argument="-db_gencode" type="select" label="Database/subject genetic code"> |
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148 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details --> |
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149 <option value="1" selected="true">1. Standard</option> |
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150 <option value="2">2. Vertebrate Mitochondrial</option> |
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151 <option value="3">3. Yeast Mitochondrial</option> |
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152 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option> |
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153 <option value="5">5. Invertebrate Mitochondrial</option> |
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154 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option> |
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155 <option value="9">9. Echinoderm Mitochondrial</option> |
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156 <option value="10">10. Euplotid Nuclear</option> |
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157 <option value="11">11. Bacteria and Archaea</option> |
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158 <option value="12">12. Alternative Yeast Nuclear</option> |
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159 <option value="13">13. Ascidian Mitochondrial</option> |
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160 <option value="14">14. Flatworm Mitochondrial</option> |
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161 <option value="15">15. Blepharisma Macronuclear</option> |
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162 <option value="16">16. Chlorophycean Mitochondrial Code</option> |
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163 <option value="21">21. Trematode Mitochondrial Code</option> |
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164 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option> |
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165 <option value="23">23. Thraustochytrium Mitochondrial Code</option> |
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166 <option value="24">24. Pterobranchia mitochondrial code</option> |
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167 </param> |
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168 </xml> |
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169 <xml name="input_conditional_nucleotide_db"> |
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170 <!-- Usually database argument mapped to -db, but -in in dustmasker --> |
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171 <conditional name="db_opts"> |
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172 <param name="db_opts_selector" type="select" label="Subject database/sequences"> |
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173 <option value="db" selected="true">Locally installed BLAST database</option> |
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174 <option value="histdb">BLAST database from your history</option> |
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175 <option value="file">FASTA file from your history (see warning note below)</option> |
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176 </param> |
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177 <when value="db"> |
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178 <param name="database" type="select" multiple="true" label="Nucleotide BLAST database"> |
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179 <options from_data_table="blastdb" /> |
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180 </param> |
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181 <param name="histdb" type="hidden" value="" /> |
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182 <param name="subject" type="hidden" value="" /> |
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183 </when> |
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184 <when value="histdb"> |
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185 <param name="database" type="hidden" value="" /> |
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186 <param name="histdb" type="data" format="blastdbn" label="Nucleotide BLAST database" /> |
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187 <param name="subject" type="hidden" value="" /> |
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188 </when> |
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189 <when value="file"> |
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190 <param name="database" type="hidden" value="" /> |
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191 <param name="histdb" type="hidden" value="" /> |
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192 <param argument="-subject" type="data" format="fasta" label="Nucleotide FASTA subject file to use instead of a database"/> |
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193 </when> |
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194 </conditional> |
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195 </xml> |
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196 <xml name="input_conditional_protein_db"> |
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197 <!-- Usually database argument mapped to -db, but -in in segmasker --> |
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198 <conditional name="db_opts"> |
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199 <param name="db_opts_selector" type="select" label="Subject database/sequences"> |
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200 <option value="db" selected="true">Locally installed BLAST database</option> |
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201 <option value="histdb">BLAST database from your history</option> |
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202 <option value="file">FASTA file from your history (see warning note below)</option> |
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203 </param> |
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204 <when value="db"> |
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205 <param name="database" type="select" multiple="true" label="Protein BLAST database"> |
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206 <options from_data_table="blastdb_p" /> |
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207 </param> |
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208 <param name="histdb" type="hidden" value="" /> |
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209 <param name="subject" type="hidden" value="" /> |
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210 </when> |
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211 <when value="histdb"> |
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212 <param name="database" type="hidden" value="" /> |
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213 <param name="histdb" type="data" format="blastdbp" label="Protein BLAST database" /> |
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214 <param name="subject" type="hidden" value="" /> |
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215 </when> |
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216 <when value="file"> |
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217 <param name="database" type="hidden" value="" /> |
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218 <param name="histdb" type="hidden" value="" /> |
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219 <param argument="-subject" type="data" format="fasta" label="Protein FASTA subject file to use instead of a database"/> |
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220 </when> |
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221 </conditional> |
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222 </xml> |
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223 <xml name="input_conditional_pssm"> |
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224 <conditional name="db_opts"> |
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225 <param name="db_opts_selector" type="select" label="Protein domain database (PSSM)"> |
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226 <option value="db" selected="true">Locally installed BLAST protein domain database</option> |
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227 <option value="histdb">BLAST protein domain database from your history</option> |
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228 </param> |
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229 <when value="db"> |
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230 <param name="database" argument="-db" type="select" label="Protein domain database"> |
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231 <options from_data_table="blastdb_d" /> |
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232 </param> |
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233 <param name="histdb" type="hidden" value="" /> |
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234 <param name="subject" type="hidden" value="" /> |
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235 </when> |
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236 <when value="histdb"> |
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237 <param name="database" type="hidden" value="" /> |
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238 <param name="histdb" argument="-db" type="data" format="blastdbd" label="Protein domain database" /> |
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239 <param name="subject" type="hidden" value="" /> |
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240 </when> |
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241 </conditional> |
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242 </xml> |
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243 <xml name="input_conditional_choose_db_type"> |
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244 <conditional name="db_opts"> |
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245 <param argument="-db_type" type="select" label="Type of BLAST database"> |
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246 <option value="nucl" selected="true">Nucleotide</option> |
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247 <option value="prot">Protein</option> |
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248 </param> |
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249 <when value="nucl"> |
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250 <param name="database" argument="-db" type="select" multiple="true" label="Nucleotide BLAST database"> |
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251 <options from_data_table="blastdb" /> |
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252 </param> |
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253 </when> |
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254 <when value="prot"> |
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255 <param name="database" argument="-db" type="select" multiple="true" label="Protein BLAST database"> |
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256 <options from_data_table="blastdb_p" /> |
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257 </param> |
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258 </when> |
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259 </conditional> |
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260 </xml> |
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261 <xml name="input_parse_deflines"> |
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262 <param argument="-parse_deflines" type="boolean" label="Should the query and subject defline(s) be parsed?" truevalue="-parse_deflines" falsevalue="" checked="false" help="This affects the formatting of the query/subject ID strings"/> |
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263 </xml> |
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264 <xml name="input_filter_query_default_false"> |
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265 <param name="filter_query" argument="-seg" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="false" /> |
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266 </xml> |
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267 <xml name="input_filter_query_default_true"> |
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268 <param name="filter_query" argument="-seg" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="true" /> |
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269 </xml> |
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270 <xml name="input_max_hits"> |
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271 <param name="max_hits" type="integer" min="0" value="0" label="Maximum hits to show" help="Use zero for default limits" /> |
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272 <param argument="-max_hsps" type="integer" min="1" optional="true" value="" label="Maximum number of HSPs (alignments) to keep for any single query-subject pair" help="The HSPs shown will be the best as judged by expect value. If this option is not set, BLAST shows all HSPs meeting the expect value criteria" /> |
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273 </xml> |
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274 <xml name="input_evalue"> |
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275 <param name="evalue_cutoff" argument="-evalue" type="float" size="15" value="0.001" label="Set expectation value cutoff" /> |
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276 </xml> |
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277 <xml name="input_word_size"> |
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278 <param argument="-word_size" type="integer" min="0" value="0" label="Word size for wordfinder algorithm" help="Use zero for default, otherwise minimum 2" /> |
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279 </xml> |
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280 <xml name="input_strand"> |
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281 <param argument="-strand" type="select" label="Query strand(s) to search against database/subject"> |
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282 <option value="-strand both">Both</option> |
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283 <option value="-strand plus">Plus (forward)</option> |
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284 <option value="-strand minus">Minus (reverse complement)</option> |
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285 </param> |
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286 </xml> |
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287 <xml name="input_qcov_hsp_perc"> |
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288 <param argument="-qcov_hsp_perc" type="float" value="0" min="0" max="100" label="Minimum query coverage per hsp (percentage, 0 to 100)" help="See also the output column qcovhsp"/> |
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289 </xml> |
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290 <xml name="advanced_options"> |
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291 <conditional name="adv_opts"> |
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292 <param name="adv_opts_selector" type="select" label="Advanced Options"> |
22
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293 <option value="basic" selected="true">Hide Advanced Options</option> |
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294 <option value="advanced">Show Advanced Options</option> |
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295 </param> |
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296 <when value="basic" /> |
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297 <when value="advanced"> |
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298 <yield /> |
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299 </when> |
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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300 </conditional> |
11
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301 </xml> |
14
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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302 <xml name="advanced_optional_id_files"> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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303 <conditional name="adv_optional_id_files_opts"> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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304 <param name="adv_optional_id_files_opts_selector" type="select" |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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305 label="Restrict search of database to a given set of ID's" |
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306 help="This feature provides a means to exclude ID's from a BLAST database search. The expectation values in the BLAST results are based upon the sequences actually searched, and not on the underlying database. Note this cannot be used when comparing against a FASTA file."> |
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307 <option value="none" selected="true">No restriction, search the entire database</option> |
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308 <option value="gilist">GI identifiers</option> |
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309 <option value="negative_gilist">Negative GI identifiers</option> |
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310 <option value="seqidlist">Sequence identifiers (SeqId's)</option> |
14
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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311 </param> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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312 <when value="none" /> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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313 <when value="gilist"> |
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314 <param argument="-gilist" type="data" format="txt" label="Restrict search of database to GI's listed in this file" |
14
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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315 help="This option is only available for database searches."/> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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316 </when> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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317 <when value="negative_gilist"> |
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318 <param argument="-negative_gilist" type="data" format="txt" label="Restrict search of database to everything except the GI's listed in this file" |
14
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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319 help="This option is only available for database searches."/> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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320 </when> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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321 <when value="seqidlist"> |
22
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322 <param argument="-seqidlist" type="data" format="txt" label=" Restrict search of database to list of SeqId's" |
14
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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323 help="This option is only available for database searches."/> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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324 </when> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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325 </conditional> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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326 </xml> |
15
c16c30e9ad5b
Uploaded v0.1.03 (internal changes); v0.1.02 (BLAST+ 2.2.30 etc)
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327 <token name="@ADV_QCOV_HSP_PERC@"> |
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328 #if float(str($adv_opts.qcov_hsp_perc)) > 0: |
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329 -qcov_hsp_perc $adv_opts.qcov_hsp_perc |
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330 #end if |
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331 </token> |
14
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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332 <token name="@ADV_ID_LIST_FILTER@"> |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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333 #if $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'negative_gilist': |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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334 -negative_gilist $adv_opts.adv_optional_id_files_opts.negative_gilist |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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335 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'gilist': |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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336 -gilist $adv_opts.adv_optional_id_files_opts.gilist |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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337 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'seqidlist': |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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338 -seqidlist $adv_opts.adv_optional_id_files_opts.seqidlist |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
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339 #end if |
2fe07f50a41e
Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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340 </token> |
11
4c4a0da938ff
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341 <token name="@THREADS@">-num_threads "\${GALAXY_SLOTS:-8}"</token> |
4c4a0da938ff
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342 <token name="@BLAST_DB_SUBJECT@"> |
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343 #if $db_opts.db_opts_selector == "db": |
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344 -db '${" ".join(str($db_opts.database.fields.path).split(","))}' |
11
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345 #elif $db_opts.db_opts_selector == "histdb": |
22
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346 -db '${os.path.join($db_opts.histdb.extra_files_path, "blastdb")}' |
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347 #else: |
22
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348 -subject '$db_opts.subject' |
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349 #end if |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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350 </token> |
22
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351 <token name="@BLAST_OUTPUT@">-out '$output1' |
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352 ##Set the extended list here so when we add things, saved workflows are not affected |
13
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353 #if str($output.out_format)=="ext": |
22
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354 -outfmt '6 std sallseqid score nident positive gaps ppos qframe sframe qseq sseq qlen slen salltitles' |
13
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355 #elif str($output.out_format)=="cols" |
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356 ##Pick your own columns. Galaxy gives us it comma separated, BLAST+ wants space separated: |
623f727cdff1
Uploaded v0.1.00, uses BLAST+ 2.2.29, allows custom column selection for tabular output - including taxonomy fields.
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357 ##TODO - Can we catch the user picking no columns and raise an error here? |
623f727cdff1
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358 #set cols = (str($output.std_cols)+","+str($output.ext_cols)+","+str($output.ids_cols)+","+str($output.misc_cols)+","+str($output.tax_cols)).replace("None", "").replace(",,", ",").replace(",", " ").strip() |
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359 -outfmt '6 $cols' |
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360 #else: |
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361 ## Note do not quote this as can be '0 -html' which is really two arguments |
13
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362 -outfmt $output.out_format |
11
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363 #end if |
4c4a0da938ff
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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364 </token> |
15
c16c30e9ad5b
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365 <token name="@ADV_FILTER_QUERY@">$adv_opts.filter_query</token> |
c16c30e9ad5b
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366 <token name="@ADV_MAX_HITS@"> |
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367 ## Need int(str(...)) because $adv_opts.max_hits is an InputValueWrapper object not a string |
15
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|
368 ## Note -max_target_seqs used to simply override -num_descriptions and -num_alignments |
c16c30e9ad5b
Uploaded v0.1.03 (internal changes); v0.1.02 (BLAST+ 2.2.30 etc)
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369 ## but this was changed in BLAST+ 2.2.27 onwards to force their use (raised with NCBI) |
11
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370 #if (str($adv_opts.max_hits) and int(str($adv_opts.max_hits)) > 0): |
22
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371 #if str($output.out_format) in ["6", "ext", "cols", "5"]: |
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372 ## Most output formats use this, including tabular and XML: |
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373 -max_target_seqs $adv_opts.max_hits |
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374 #else |
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375 ## Text and HTML output formats 0-4 currently need this instead: |
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376 -num_descriptions $adv_opts.max_hits -num_alignments $adv_opts.max_hits |
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377 #end if |
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378 #end if |
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379 #if str($adv_opts.max_hsps) |
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380 -max_hsps $adv_opts.max_hsps |
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381 #end if |
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382 </token> |
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383 <token name="@ADV_WORD_SIZE@"> |
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384 #if (str($adv_opts.word_size) and int(str($adv_opts.word_size)) > 0): |
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385 -word_size $adv_opts.word_size |
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386 #end if |
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387 $adv_opts.parse_deflines |
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388 </token> |
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389 <!-- @ON_DB_SUBJECT@ is for use with @BLAST_DB_SUBJECT@ --> |
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390 <token name="@ON_DB_SUBJECT@">#if str($db_opts.db_opts_selector)=='db' |
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391 '${db_opts.database}' |
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392 #elif str($db_opts.db_opts_selector)=='histdb' |
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393 '${db_opts.histdb.name}' |
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394 #else |
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395 '${db_opts.subject.name}' |
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396 #end if</token> |
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397 <token name="@REFERENCES@"> |
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398 Peter J. A. Cock, John M. Chilton, Björn Grüning, James E. Johnson, Nicola Soranzo (2015). |
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399 NCBI BLAST+ integrated into Galaxy. *GigaScience* 4:39 |
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400 http://dx.doi.org/10.1186/s13742-015-0080-7 |
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401 |
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402 Christiam Camacho et al. (2009). |
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403 BLAST+: architecture and applications. |
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404 *BMC Bioinformatics* 15;10:421. |
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405 http://dx.doi.org/10.1186/1471-2105-10-421 |
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406 |
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407 This wrapper is available to install into other Galaxy Instances via the Galaxy |
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408 Tool Shed at http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus |
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409 </token> |
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410 <xml name="blast_citations"> |
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411 <citations> |
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412 <citation type="doi">10.1186/1471-2105-10-421</citation> |
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413 <citation type="doi">10.1186/s13742-015-0080-7</citation> |
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414 </citations> |
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415 </xml> |
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416 <token name="@OUTPUT_FORMAT@">**Output format** |
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417 |
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418 Because Galaxy focuses on processing tabular data, the default output of this |
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419 tool is tabular. The standard BLAST+ tabular output contains 12 columns: |
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420 |
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421 ====== ========= ============================================ |
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422 Column NCBI name Description |
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423 ------ --------- -------------------------------------------- |
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424 1 qseqid Query Seq-id (ID of your sequence) |
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425 2 sseqid Subject Seq-id (ID of the database hit) |
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426 3 pident Percentage of identical matches |
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427 4 length Alignment length |
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428 5 mismatch Number of mismatches |
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429 6 gapopen Number of gap openings |
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430 7 qstart Start of alignment in query |
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431 8 qend End of alignment in query |
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432 9 sstart Start of alignment in subject (database hit) |
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433 10 send End of alignment in subject (database hit) |
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434 11 evalue Expectation value (E-value) |
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435 12 bitscore Bit score |
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436 ====== ========= ============================================ |
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437 |
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438 The BLAST+ tools can optionally output additional columns of information, |
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439 but this takes longer to calculate. Many commonly used extra columns are |
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440 included by selecting the extended tabular output. The extra columns are |
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441 included *after* the standard 12 columns. This is so that you can write |
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442 workflow filtering steps that accept either the 12 or 25 column tabular |
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443 BLAST output. Galaxy now uses this extended 25 column output by default. |
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444 |
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445 ====== ============= =========================================== |
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446 Column NCBI name Description |
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447 ------ ------------- ------------------------------------------- |
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448 13 sallseqid All subject Seq-id(s), separated by a ';' |
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449 14 score Raw score |
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450 15 nident Number of identical matches |
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451 16 positive Number of positive-scoring matches |
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452 17 gaps Total number of gaps |
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453 18 ppos Percentage of positive-scoring matches |
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454 19 qframe Query frame |
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455 20 sframe Subject frame |
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456 21 qseq Aligned part of query sequence |
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457 22 sseq Aligned part of subject sequence |
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458 23 qlen Query sequence length |
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459 24 slen Subject sequence length |
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460 25 salltitles All subject title(s), separated by a '<>' |
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461 ====== ============= =========================================== |
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462 |
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463 The third option is to customise the tabular output by selecting which |
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464 columns you want, from the standard set of 12, the default set of 25, |
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465 or any of the additional columns BLAST+ offers (including species name). |
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466 |
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467 The fourth option is BLAST XML output, which is designed to be parsed by |
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468 another program, and is understood by some Galaxy tools. |
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469 |
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470 You can also choose several plain text or HTML output formats which are designed to be read by a person (not by another program). |
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471 The HTML versions use basic webpage formatting and can include links to the hits on the NCBI website. |
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472 The pairwise output (the default on the NCBI BLAST website) shows each match as a pairwise alignment with the query. |
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473 The two query anchored outputs show a multiple sequence alignment between the query and all the matches, |
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474 and differ in how insertions are shown (marked as insertions or with gap characters added to the other sequences). |
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475 </token> |
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476 <token name="@FASTA_WARNING@">.. class:: warningmark |
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477 |
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478 You can also search against a FASTA file of subject (target) |
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479 sequences. This is *not* advised because it is slower (only one |
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480 CPU is used), but more importantly gives e-values for pairwise |
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481 searches (very small e-values which will look overly signficiant). |
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482 In most cases you should instead turn the other FASTA file into a |
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483 database first using *makeblastdb* and search against that. |
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484 </token> |
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485 <token name="@SEARCH_TIME_WARNING@">.. class:: warningmark |
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486 |
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487 **Note**. Database searches may take a substantial amount of time. |
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488 For large input datasets it is advisable to allow overnight processing. |
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489 |
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490 ----- |
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491 </token> |
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492 </macros> |