Mercurial > repos > devteam > ncbi_blast_plus
annotate tools/ncbi_blast_plus/ncbi_blastp_wrapper.xml @ 35:cbf3f518b668 draft default tip
planemo upload for repository https://github.com/peterjc/galaxy_blast/tree/master/tools/ncbi_blast_plus commit 45f13845a19ba74f7a6284cc06371ec64213d832
author | peterjc |
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date | Thu, 22 Feb 2024 15:07:36 +0000 |
parents | a52d2d93e595 |
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rev | line source |
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"planemo upload for repository https://github.com/peterjc/galaxy_blast/tree/master/tools/ncbi_blast_plus commit 3f9f39ad808325a11d9967980d2cb82c96d69324"
peterjc
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1 <tool id="ncbi_blastp_wrapper" name="NCBI BLAST+ blastp" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> |
3
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2 <description>Search protein database with protein query sequence(s)</description> |
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3 <macros> |
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4 <token name="@BINARY@">blastp</token> |
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5 <import>ncbi_macros.xml</import> |
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6 </macros> |
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v0.1.05 - Update citation information now GigaScience paper is out
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7 <expand macro="parallelism" /> |
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8 <expand macro="preamble" /> |
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9 <command detect_errors="aggressive"> |
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10 ## The command is a Cheetah template which allows some Python based syntax. |
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11 ## Lines starting hash hash are comments. Galaxy will turn newlines into spaces |
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12 blastp |
25 | 13 @QUERY@ |
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14 @BLAST_DB_SUBJECT@ |
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15 -task $blast_type |
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16 -evalue $evalue_cutoff |
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17 @BLAST_OUTPUT@ |
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18 @THREADS@ |
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19 #if $adv_opts.adv_opts_selector=="advanced": |
23 | 20 @ADV_MATRIX_GAPCOSTS@ |
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21 @ADV_FILTER_QUERY@ |
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22 @ADV_MAX_HITS@ |
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23 @ADV_WORD_SIZE@ |
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24 ##Ungapped disabled for now - see comments below |
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25 ##$adv_opts.ungapped |
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26 @ADV_ID_LIST_FILTER@ |
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27 @ADV_QCOV_HSP_PERC@ |
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28 $adv_opts.use_sw_tback |
23 | 29 @ADV_WINDOW_SIZE@ |
30 @ADV_THRESHOLD@ | |
31 @ADV_COMP_BASED_STATS@ | |
32 ## End of advanced options: | |
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33 #end if |
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34 </command> |
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35 <inputs> |
25 | 36 <expand macro="prot_query" /> |
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37 |
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38 <expand macro="input_conditional_protein_db" /> |
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39 |
23 | 40 <param name="blast_type" argument="-task" type="select" display="radio" |
41 label="Type of BLAST" | |
42 help="See help text for default parameter values for each BLAST type."> | |
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43 <option value="blastp">blastp - Traditional BLASTP to compare a protein query to a protein database</option> |
23 | 44 <option value="blastp-short">blastp-short - BLASTP optimized for queries shorter than 30 residues</option> |
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45 <option value="blastp-fast">blastp-fast - Use longer words for seeding, faster but less accurate</option> |
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46 </param> |
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47 <expand macro="input_evalue" /> |
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48 <expand macro="input_out_format" /> |
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49 <expand macro="advanced_options"> |
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50 <!-- Could use a select (yes, no, other) where other allows setting 'window locut hicut' --> |
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51 <expand macro="input_filter_query_default_false" /> |
23 | 52 <expand macro="input_matrix_gapcosts" /> |
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53 <expand macro="input_max_hits" /> |
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54 <expand macro="input_word_size" /> |
23 | 55 <expand macro="input_window_size" /> |
56 <expand macro="input_threshold" /> | |
57 <expand macro="input_comp_based_stats" /> | |
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58 <!-- |
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59 Can't use '-ungapped' on its own, error back is: |
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60 Composition-adjusted searched are not supported with an ungapped search, please add -comp_based_stats F or do a gapped search |
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61 Tried using '-ungapped -comp_based_stats F' and blastp crashed with 'Attempt to access NULL pointer.' |
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62 <param argument="-ungapped" type="boolean" label="Perform ungapped alignment only?" truevalue="-ungapped -comp_based_stats F" falsevalue="" checked="false" /> |
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63 --> |
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64 <expand macro="input_parse_deflines" /> |
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Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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65 <expand macro="advanced_optional_id_files" /> |
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66 <expand macro="input_qcov_hsp_perc" /> |
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67 <param argument="-use_sw_tback" type="boolean" truevalue="-use_sw_tback" falsevalue="" label="Compute locally optimal Smith-Waterman alignments" /> |
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68 </expand> |
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69 </inputs> |
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70 <outputs> |
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71 <data name="output1" format="tabular" label="${blast_type.value} $query.name vs @ON_DB_SUBJECT@"> |
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72 <expand macro="output_change_format" /> |
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73 </data> |
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74 </outputs> |
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75 <tests> |
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76 <test> |
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77 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
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78 <param name="db_opts_selector" value="file" /> |
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79 <param name="subject" value="rhodopsin_proteins.fasta" ftype="fasta" /> |
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80 <param name="database" value="" /> |
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81 <param name="evalue_cutoff" value="1e-8" /> |
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82 <param name="blast_type" value="blastp" /> |
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83 <param name="out_format" value="5" /> |
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84 <param name="adv_opts_selector" value="advanced" /> |
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85 <param name="filter_query" value="false" /> |
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86 <param name="matrix" value="BLOSUM62" /> |
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87 <param name="max_hits" value="0" /> |
23 | 88 <param name="word_size" value="" /> |
89 <param name="window_size" value="40" /> | |
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90 <param name="parse_deflines" value="true" /> |
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91 <param name="qcov_hsp_perc" value="25" /> |
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92 <output name="output1" file="blastp_four_human_vs_rhodopsin.xml" ftype="blastxml" /> |
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93 </test> |
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94 <test> |
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95 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
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96 <param name="db_opts_selector" value="file" /> |
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97 <param name="subject" value="rhodopsin_proteins.fasta" ftype="fasta" /> |
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98 <param name="database" value="" /> |
643338ac83c0
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99 <param name="evalue_cutoff" value="1e-8" /> |
643338ac83c0
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100 <param name="blast_type" value="blastp" /> |
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101 <param name="out_format" value="6" /> |
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102 <param name="adv_opts_selector" value="advanced" /> |
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103 <param name="filter_query" value="false" /> |
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104 <param name="matrix" value="BLOSUM62" /> |
643338ac83c0
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105 <param name="max_hits" value="0" /> |
23 | 106 <param name="word_size" value="" /> |
107 <param name="window_size" value="40" /> | |
22
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108 <param name="parse_deflines" value="true" /> |
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109 <param name="qcov_hsp_perc" value="25" /> |
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110 <output name="output1" file="blastp_four_human_vs_rhodopsin.tabular" ftype="tabular" /> |
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111 </test> |
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112 <test> |
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113 <param name="query" value="four_human_proteins.fasta" ftype="fasta" /> |
643338ac83c0
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114 <param name="db_opts_selector" value="file" /> |
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115 <param name="subject" value="rhodopsin_proteins.fasta" ftype="fasta" /> |
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116 <param name="database" value="" /> |
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117 <param name="evalue_cutoff" value="1e-8" /> |
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118 <param name="blast_type" value="blastp" /> |
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119 <param name="out_format" value="ext" /> |
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120 <param name="adv_opts_selector" value="advanced" /> |
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121 <param name="filter_query" value="false" /> |
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122 <param name="matrix" value="BLOSUM62" /> |
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123 <param name="max_hits" value="0" /> |
23 | 124 <param name="word_size" value="" /> |
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125 <param name="parse_deflines" value="true" /> |
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126 <param name="qcov_hsp_perc" value="25" /> |
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127 <output name="output1" file="blastp_four_human_vs_rhodopsin_ext.tabular" ftype="tabular" /> |
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128 </test> |
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129 <test> |
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130 <param name="query" value="rhodopsin_proteins.fasta" ftype="fasta" /> |
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131 <param name="db_opts_selector" value="file" /> |
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132 <param name="subject" value="four_human_proteins.fasta" ftype="fasta" /> |
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133 <param name="database" value="" /> |
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134 <param name="evalue_cutoff" value="1e-8" /> |
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135 <param name="blast_type" value="blastp" /> |
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136 <param name="out_format" value="6" /> |
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137 <param name="adv_opts_selector" value="basic" /> |
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138 <output name="output1" file="blastp_rhodopsin_vs_four_human.tabular" ftype="tabular" /> |
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139 </test> |
23 | 140 <test> |
141 <param name="query" value="rhodopsin_peptides.fasta" ftype="fasta" /> | |
142 <param name="db_opts_selector" value="file" /> | |
143 <param name="subject" value="four_human_proteins.fasta" ftype="fasta" /> | |
144 <param name="database" value="" /> | |
145 <param name="evalue_cutoff" value="200000" /> | |
146 <param name="blast_type" value="blastp-short" /> | |
147 <param name="out_format" value="6" /> | |
148 <param name="adv_opts_selector" value="basic" /> | |
149 <output name="output1" file="blastp_rhodopsin_peptides_vs_four_human.tabular" ftype="tabular" /> | |
150 </test> | |
151 <test> | |
152 <param name="query" value="rhodopsin_proteins.fasta" ftype="fasta" /> | |
153 <param name="db_opts_selector" value="file" /> | |
154 <param name="subject" value="four_human_proteins.fasta" ftype="fasta" /> | |
32 | 155 <param name="database" value="" /> |
23 | 156 <param name="evalue_cutoff" value="1e-8"/> |
157 <param name="blast_type" value="blastp" /> | |
158 <param name="out_format" value="6"/> | |
159 <param name="adv_opts_selector" value="advanced"/> | |
160 <param name="window_size" value="35" /> | |
161 <param name="matrix" value="BLOSUM62" /> | |
162 <param name="gap_costs" value="-gapopen 11 -gapextend 1"/> | |
163 <param name="comp_based_stats" value="3" /> | |
164 <output name="output1" file="blastp_rhodopsin_adv_vs_four_human.tabular" ftype="tabular" /> | |
165 </test> | |
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166 </tests> |
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167 <help> |
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168 |
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169 @SEARCH_TIME_WARNING@ |
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170 |
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171 **What it does** |
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172 |
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173 Search a *protein database* using a *protein query*, |
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174 using the NCBI BLAST+ blastp command line tool. |
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175 |
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176 @FASTA_WARNING@ |
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177 |
23 | 178 ------- |
3
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179 |
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180 @OUTPUT_FORMAT@ |
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181 |
23 | 182 ------ |
183 | |
184 @CLI_OPTIONS@ | |
185 | |
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186 ------- |
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187 |
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188 **References** |
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189 |
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190 If you use this Galaxy tool in work leading to a scientific publication please |
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191 cite the following papers: |
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192 |
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193 @REFERENCES@ |
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194 </help> |
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195 <expand macro="blast_citations" /> |
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196 </tool> |