comparison tools/ncbi_blast_plus/ncbi_blastn_wrapper.xml @ 6:a23b0627623c draft

Uploaded v0.0.15, clearer warning about searching against a FASTA file.
author peterjc
date Thu, 24 Jan 2013 12:40:41 -0500
parents 393a7a35383c
children 1f546099212f
comparison
equal deleted inserted replaced
5:393a7a35383c 6:a23b0627623c
1 <tool id="ncbi_blastn_wrapper" name="NCBI BLAST+ blastn" version="0.0.14"> 1 <tool id="ncbi_blastn_wrapper" name="NCBI BLAST+ blastn" version="0.0.15">
2 <description>Search nucleotide database with nucleotide query sequence(s)</description> 2 <description>Search nucleotide database with nucleotide query sequence(s)</description>
3 <!-- If job splitting is enabled, break up the query file into parts --> 3 <!-- If job splitting is enabled, break up the query file into parts -->
4 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" shared_inputs="subject,histdb" merge_outputs="output1"></parallelism> 4 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" shared_inputs="subject,histdb" merge_outputs="output1"></parallelism>
5 <version_command>blastn -version</version_command> 5 <version_command>blastn -version</version_command>
6 <command> 6 <command>
53 <param name="query" type="data" format="fasta" label="Nucleotide query sequence(s)"/> 53 <param name="query" type="data" format="fasta" label="Nucleotide query sequence(s)"/>
54 <conditional name="db_opts"> 54 <conditional name="db_opts">
55 <param name="db_opts_selector" type="select" label="Subject database/sequences"> 55 <param name="db_opts_selector" type="select" label="Subject database/sequences">
56 <option value="db" selected="True">BLAST Database</option> 56 <option value="db" selected="True">BLAST Database</option>
57 <option value="histdb">BLAST database from your history</option> 57 <option value="histdb">BLAST database from your history</option>
58 <option value="file">FASTA file from your history (pairwise e-values)</option> 58 <option value="file">FASTA file from your history (see warning note below)</option>
59 </param> 59 </param>
60 <when value="db"> 60 <when value="db">
61 <param name="database" type="select" label="Nucleotide BLAST database"> 61 <param name="database" type="select" label="Nucleotide BLAST database">
62 <options from_file="blastdb.loc"> 62 <options from_file="blastdb.loc">
63 <column name="value" index="0"/> 63 <column name="value" index="0"/>
160 160
161 Search a *nucleotide database* using a *nucleotide query*, 161 Search a *nucleotide database* using a *nucleotide query*,
162 using the NCBI BLAST+ blastn command line tool. 162 using the NCBI BLAST+ blastn command line tool.
163 Algorithms include blastn, megablast, and discontiguous megablast. 163 Algorithms include blastn, megablast, and discontiguous megablast.
164 164
165 .. class:: warningmark
166
167 You can also search against a FASTA file of subject nucleotide
168 sequences. This is *not* advised because it is slower (only one
169 CPU is used), but more importantly gives e-values for pairwise
170 searches (very small e-values which will look overly signficiant).
171 In most cases you should instead turn the other FASTA file into a
172 database first using *makeblastdb* and search against that.
173
165 ----- 174 -----
166 175
167 **Output format** 176 **Output format**
168 177
169 Because Galaxy focuses on processing tabular data, the default output of this 178 Because Galaxy focuses on processing tabular data, the default output of this