Mercurial > repos > devteam > ncbi_blast_plus
view tools/ncbi_blast_plus/check_no_duplicates.py @ 11:4c4a0da938ff draft
Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
Supports $GALAXY_SLOTS.
Includes more tests and heavy use of macros.
author | peterjc |
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date | Thu, 05 Dec 2013 06:55:59 -0500 |
parents | |
children | 3034ce97dd33 |
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#!/usr/bin/env python """Check for duplicate sequence identifiers in FASTA files. This is run as a pre-check before makeblastdb, in order to avoid a regression bug in BLAST+ 2.2.28 which fails to catch this. See: http://blastedbio.blogspot.co.uk/2012/10/my-ids-not-good-enough-for-ncbi-blast.html This script takes one or more FASTA filenames as input, and will return a non-zero error if any duplicate identifiers are found. """ import sys import os if "-v" in sys.argv or "--version" in sys.argv: print("v0.0.22") sys.exit(0) def stop_err(msg, error=1): sys.stderr.write("%s\n" % msg) sys.exit(error) identifiers = set() files = 0 for filename in sys.argv[1:]: if not os.path.isfile(filename): stop_err("Missing FASTA file %r" % filename, 2) files += 1 handle = open(filename) for line in handle: if line.startswith(">"): #The split will also take care of the new line character, #e.g. ">test\n" and ">test description here\n" both give "test" seq_id = line[1:].split(None, 1)[0] if seq_id in identifiers: handle.close() stop_err("Repeated identifiers, e.g. %r" % seq_id, 1) identifiers.add(seq_id) handle.close() if not files: stop_err("No FASTA files given to check for duplicates", 3) elif files == 1: print("%i sequences" % len(identifiers)) else: print("%i sequences in %i FASTA files" % (len(identifiers), files))