view tools/ncbi_blast_plus/ncbi_dustmasker_wrapper.xml @ 13:623f727cdff1 draft

Uploaded v0.1.00, uses BLAST+ 2.2.29, allows custom column selection for tabular output - including taxonomy fields.

<tool id="ncbi_dustmasker_wrapper" name="NCBI BLAST+ dustmasker" version="0.1.00">
    <!-- dustmasker wrapper from Edward Kirton and Nicola Soranzo -->
    <description>masks low complexity regions</description>
    <macros>
        <token name="@BINARY@">dustmasker</token>
        <import>ncbi_macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <command>
## The command is a Cheetah template which allows some Python based syntax.
## Lines starting hash hash are comments. Galaxy will turn newlines into spaces
dustmasker
#if $db_opts.db_opts_selector == "db":
  -in "${db_opts.database.fields.path}" -infmt blastdb
#elif $db_opts.db_opts_selector == "histdb":
  -in "${os.path.join($db_opts.histdb.extra_files_path, 'blastdb')}" -infmt blastdb
#else:
  -in "$subject" -infmt fasta
#end if
-out "$outfile"
-window $window -level $level -linker $linker -outfmt $outformat
    </command>
    <expand macro="stdio" />
    <inputs>
        <expand macro="input_conditional_nucleotide_db" />
        <param name="window" type="integer" value="64" label="DUST window length" />
        <param name="level" type="integer" value="20" label="DUST level" help="Score threshold for subwindows" />
        <param name="linker" type="integer" value="1" label="DUST linker" help="How close masked intervals should be to get merged together" />
        <param name="outformat" type="select" label="Output format">
            <!-- seqloc_* formats are not very useful
                 and what BLAST+ calls 'interval' is not what Galaxy calls interval format
            -->
            <option value="fasta">FASTA</option>
            <option value="maskinfo_asn1_bin">maskinfo ASN.1 binary</option>
            <option value="maskinfo_asn1_text" selected="true">maskinfo ASN.1 text</option>
            <option value="maskinfo_xml">maskinfo_xml</option>
        </param>
    </inputs>
    <outputs>
        <data name="outfile" format="maskinfo-asn1" label="DUST Masked File">
            <change_format>
                <when input="outformat" value="fasta" format="fasta" />
                <when input="outformat" value="maskinfo_asn1_bin" format="maskinfo-asn1-binary" />
		<!--
                <when input="outformat" value="maskinfo_asn1_text" format="maskinfo-asn1" />
		-->
                <when input="outformat" value="maskinfo_xml" format="xml" />
            </change_format>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="three_human_mRNA.fasta" ftype="fasta" />
            <param name="window" value="64" />
            <param name="level" value="20" />
            <param name="linker" value="1" />
            <param name="outformat" value="fasta" />
            <output name="outfile" file="dustmasker_three_human.fasta" />
        </test>
        <test>
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="three_human_mRNA.fasta" ftype="fasta" />
            <param name="window" value="64" />
            <param name="level" value="20" />
            <param name="linker" value="1" />
            <param name="outformat" value="maskinfo_asn1_bin" />
            <output name="outfile" file="dustmasker_three_human.maskinfo-asn1-binary" />
        </test>
        <test>
            <param name="db_opts_selector" value="file" />
            <param name="subject" value="three_human_mRNA.fasta" ftype="fasta" />
            <param name="window" value="64" />
            <param name="level" value="20" />
            <param name="linker" value="1" />
            <param name="outformat" value="maskinfo_asn1_text" />
            <output name="outfile" file="dustmasker_three_human.maskinfo-asn1" />
        </test>
    </tests>
    <help>
**What it does**

This tool identifies and masks out low complexity regions of a nucleotide database (or sequences in FASTA format) by using the symmetric DUST_ algorithm.

If you select *maskinfo ASN.1* (binary or text) as output format, the output file can be used as masking data for NCBI BLAST+ makeblastdb tool.

More information about dustmasker can be found in the `BLAST Command Line Applications User Manual`_.

.. _BLAST Command Line Applications User Manual: http://www.ncbi.nlm.nih.gov/books/NBK1763/
.. _DUST: http://www.ncbi.nlm.nih.gov/pubmed/16796549

**References**

If you use this Galaxy tool in work leading to a scientific publication please
cite the following papers (a more specific paper covering this wrapper is planned):

@REFERENCES@
    </help>
</tool>