Galaxy tool MarkDuplicates run at 12/05/2011 14:34:29
The following output files were created (click the filename to view/download a copy):
Picard on line resources
Picard output (transposed to make it easier to see)
## net.sf.picard.metrics.StringHeader |
# net.sf.picard.sam.MarkDuplicates INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true ASSUME_SORTED=true READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 TMP_DIR=/tmp/rerla VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false |
## net.sf.picard.metrics.StringHeader |
# Started on: Thu May 12 14:34:28 EDT 2011 |
## METRICS CLASS net.sf.picard.sam.DuplicationMetrics |
LIBRARY | |
UNPAIRED_READS_EXAMINED | 1 |
READ_PAIRS_EXAMINED | 3 |
UNMAPPED_READS | 1 |
UNPAIRED_READ_DUPLICATES | 1 |
READ_PAIR_DUPLICATES | 1 |
READ_PAIR_OPTICAL_DUPLICATES | 0 |
PERCENT_DUPLICATION | 0.428571 |
ESTIMATED_LIBRARY_SIZE
| 3
|
## HISTOGRAM java.lang.Double |
BIN VALUE |
1.0 0.948181 |
2.0 1.296997 |
3.0 1.425319 |
4.0 1.472527 |
5.0 1.489893 |
6.0 1.496282 |
7.0 1.498632 |
8.0 1.499497 |
9.0 1.499815 |
10.0 1.499932 |
11.0 1.499975 |
12.0 1.499991 |
13.0 1.499997 |
14.0 1.499999 |
15.0 1.5 |
16.0 1.5 |
17.0 1.5 |
18.0 1.5 |
19.0 1.5 |
20.0 1.5 |
21.0 1.5 |
22.0 1.5 |
23.0 1.5 |
24.0 1.5 |
25.0 1.5 |
26.0 1.5 |
27.0 1.5 |
28.0 1.5 |
29.0 1.5 |
30.0 1.5 |
31.0 1.5 |
32.0 1.5 |
33.0 1.5 |
34.0 1.5 |
35.0 1.5 |
36.0 1.5 |
37.0 1.5 |
38.0 1.5 |
39.0 1.5 |
40.0 1.5 |
41.0 1.5 |
42.0 1.5 |
43.0 1.5 |
44.0 1.5 |
45.0 1.5 |
46.0 1.5 |
47.0 1.5 |
48.0 1.5 |
49.0 1.5 |
50.0 1.5 |
51.0 1.5 |
52.0 1.5 |
53.0 1.5 |
54.0 1.5 |
55.0 1.5 |
56.0 1.5 |
57.0 1.5 |
58.0 1.5 |
59.0 1.5 |
60.0 1.5 |
61.0 1.5 |
62.0 1.5 |
63.0 1.5 |
64.0 1.5 |
65.0 1.5 |
66.0 1.5 |
67.0 1.5 |
68.0 1.5 |
69.0 1.5 |
70.0 1.5 |
71.0 1.5 |
72.0 1.5 |
73.0 1.5 |
74.0 1.5 |
75.0 1.5 |
76.0 1.5 |
77.0 1.5 |
78.0 1.5 |
79.0 1.5 |
80.0 1.5 |
81.0 1.5 |
82.0 1.5 |
83.0 1.5 |
84.0 1.5 |
85.0 1.5 |
86.0 1.5 |
87.0 1.5 |
88.0 1.5 |
89.0 1.5 |
90.0 1.5 |
91.0 1.5 |
92.0 1.5 |
93.0 1.5 |
94.0 1.5 |
95.0 1.5 |
96.0 1.5 |
97.0 1.5 |
98.0 1.5 |
99.0 1.5 |
Picard Tool Run Log
Thu, 12 May 2011 14:34:29 INFO
## executing java -Xmx2g -jar /udd/rerla/galaxy-central/tool-data/shared/jars/MarkDuplicates.jar VALIDATION_STRINGENCY=LENIENT ASSUME_SORTED=true INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true READ_NAME_REGEX="[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).*" OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 returned status 0 and stderr:
[Thu May 12 14:34:28 EDT 2011] net.sf.picard.sam.MarkDuplicates INPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1097.dat OUTPUT=/tmp/6729253.1.all.q/tmpM5wI_h/database/files/001/dataset_1098.dat METRICS_FILE=/udd/rerla/galaxy-central/database/job_working_directory/1032/dataset_1099_files/MarkDuplicates.metrics.txt REMOVE_DUPLICATES=true ASSUME_SORTED=true READ_NAME_REGEX=[a-zA-Z0-9]+:[0-9]:([0-9]+):([0-9]+):([0-9]+).* OPTICAL_DUPLICATE_PIXEL_DISTANCE=100 VALIDATION_STRINGENCY=LENIENT MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP=50000 MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=8000 TMP_DIR=/tmp/rerla VERBOSITY=INFO QUIET=false COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false
INFO 2011-05-12 14:34:28 MarkDuplicates Start of doWork freeMemory: 8644632; totalMemory: 9109504; maxMemory: 1908932608
INFO 2011-05-12 14:34:28 MarkDuplicates Reading input file and constructing read end information.
INFO 2011-05-12 14:34:28 MarkDuplicates Will retain up to 7575129 data points before spilling to disk.
INFO 2011-05-12 14:34:28 MarkDuplicates Read 7 records. 0 pairs never matched.
INFO 2011-05-12 14:34:29 MarkDuplicates After buildSortedReadEndLists freeMemory: 8316168; totalMemory: 130351104; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates Will retain up to 59654144 duplicate indices before spilling to disk.
INFO 2011-05-12 14:34:29 MarkDuplicates Traversing read pair information and detecting duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Traversing fragment information and detecting duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Sorting list of duplicate records.
INFO 2011-05-12 14:34:29 MarkDuplicates After generateDuplicateIndexes freeMemory: 129615120; totalMemory: 607649792; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates Marking 3 records as duplicates.
INFO 2011-05-12 14:34:29 MarkDuplicates Found 0 optical duplicate clusters.
INFO 2011-05-12 14:34:29 MarkDuplicates Before output close freeMemory: 128997520; totalMemory: 607649792; maxMemory: 1908932608
INFO 2011-05-12 14:34:29 MarkDuplicates After output close freeMemory: 128997280; totalMemory: 607649792; maxMemory: 1908932608
[Thu May 12 14:34:29 EDT 2011] net.sf.picard.sam.MarkDuplicates done.
Runtime.totalMemory()=607649792
The freely available
Picard software
generated all outputs reported here running as a
Galaxy tool