Mercurial > repos > devteam > sam_pileup
diff sam_pileup.xml @ 4:a3b4ad6858ff draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/sam_pileup commit 8301d37348be25a038b3c63b049b1178d05f5003"
| author | devteam |
|---|---|
| date | Thu, 06 Feb 2020 07:11:33 -0500 |
| parents | 3ff8935743a9 |
| children |
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--- a/sam_pileup.xml Thu Jan 09 14:28:39 2014 -0500 +++ b/sam_pileup.xml Thu Feb 06 07:11:33 2020 -0500 @@ -1,124 +1,126 @@ -<tool id="sam_pileup" name="Generate pileup" version="1.1.2"> - <description>from BAM dataset</description> - <requirements> - <requirement type="package" version="0.1.16">samtools</requirement> - </requirements> - <command interpreter="python"> - sam_pileup.py - --input1=$input1 - --output=$output1 - --ref=$refOrHistory.reference - #if $refOrHistory.reference == "history": - --ownFile=$refOrHistory.ownFile - #else: - --index=${refOrHistory.index.fields.path} - #end if - --bamIndex=${input1.metadata.bam_index} - --lastCol=$lastCol - --indels=$indels - --mapCap=$mapCap - --consensus=$c.consensus - #if $c.consensus == "yes": - --theta=$c.theta - --hapNum=$c.hapNum - --fraction=$c.fraction - --phredProb=$c.phredProb - #else: - --theta="None" - --hapNum="None" - --fraction="None" - --phredProb="None" - #end if - </command> - <inputs> - <conditional name="refOrHistory"> - <param name="reference" type="select" label="Will you select a reference genome from your history or use a built-in index?"> - <option value="indexed">Use a built-in index</option> - <option value="history">Use one from the history</option> - </param> - <when value="indexed"> - <param name="input1" type="data" format="bam" label="Select the BAM file to generate the pileup file for"> - <validator type="unspecified_build" /> - <validator type="dataset_metadata_in_data_table" table_name="fasta_indexes" metadata_name="dbkey" metadata_column="1" message="Sequences are not currently available for the specified build." /> - - </param> - <param name="index" type="select" label="Using reference genome"> - <options from_data_table="fasta_indexes"> - <filter type="data_meta" ref="input1" key="dbkey" column="1" /> - <validator type="no_options" message="No reference genome is available for the build associated with the selected input dataset" /> - </options> +<tool id="sam_pileup" name="Generate pileup" version="1.1.3" profile="16.04"> + <description>from BAM dataset</description> + <requirements> + <requirement type="package" version="0.1.16">samtools</requirement> + </requirements> + <command><![CDATA[ +ln -s '$input1' input1.bam && +ln -s '${input1.metadata.bam_index}' 'input1.bam.bai' && +#if $refOrHistory.reference == 'history': + ln -s '$refOrHistory.ownFile' reference.fasta && + samtools faidx reference.fasta && +#end if +samtools pileup +#if $lastCol == 'yes': + -s +#end if +#if $indels == 'yes': + -i +#end if +-M $mapCap +#if $c.consensus == 'yes': + -c + -T $c.theta + -N $c.hapNum + -r $c.fraction + -I $c.phredProb +#end if +-f +#if $refOrHistory.reference == 'indexed': + '${refOrHistory.index.fields.path}' +#else: + reference.fasta +#end if +input1.bam +> '$output1' + ]]></command> + <inputs> + <conditional name="refOrHistory"> + <param name="reference" type="select" label="Will you select a reference genome from your history or use a built-in index?"> + <option value="indexed">Use a built-in index</option> + <option value="history">Use one from the history</option> + </param> + <when value="indexed"> + <param name="input1" type="data" format="bam" label="Select the BAM file to generate the pileup file for"> + <validator type="unspecified_build" /> + <validator type="dataset_metadata_in_data_table" table_name="fasta_indexes" metadata_name="dbkey" metadata_column="1" message="Sequences are not currently available for the specified build." /> + </param> + <param name="index" type="select" label="Using reference genome"> + <options from_data_table="fasta_indexes"> + <filter type="data_meta" ref="input1" key="dbkey" column="1" /> + <validator type="no_options" message="No reference genome is available for the build associated with the selected input dataset" /> + </options> + </param> + </when> + <when value="history"> + <param name="input1" type="data" format="bam" label="Select the BAM file to generate the pileup file for" /> + <param name="ownFile" argument="-f" type="data" format="fasta" label="Select a reference genome" /> + </when> + </conditional> + <param name="lastCol" argument="-s" type="select" label="Whether or not to print the mapping quality as the last column" help="Makes the output easier to parse, but is space inefficient"> + <option value="no">Do not print the mapping quality as the last column</option> + <option value="yes">Print the mapping quality as the last column</option> </param> - </when> - <when value="history"> - <param name="input1" type="data" format="bam" label="Select the BAM file to generate the pileup file for" /> - <param name="ownFile" type="data" format="fasta" metadata_name="dbkey" label="Select a reference genome" /> - </when> - </conditional> - <param name="lastCol" type="select" label="Whether or not to print the mapping quality as the last column" help="Makes the output easier to parse, but is space inefficient"> - <option value="no">Do not print the mapping quality as the last column</option> - <option value="yes">Print the mapping quality as the last column</option> - </param> - <param name="indels" type="select" label="Whether or not to print only output pileup lines containing indels"> - <option value="no">Print all lines</option> - <option value="yes">Print only lines containing indels</option> - </param> - <param name="mapCap" type="integer" value="60" label="Where to cap mapping quality" /> - <conditional name="c"> - <param name="consensus" type="select" label="Call consensus according to MAQ model?"> - <option selected="true" value="no">No</option> - <option value="yes">Yes</option> - </param> - <when value="no" /> - <when value="yes"> - <param name="theta" type="float" value="0.85" label="Theta parameter (error dependency coefficient) in the MAQ consensus calling model" /> - <param name="hapNum" type="integer" value="2" label="Number of haplotypes in the sample" help="Greater than or equal to 2" /> - <param name="fraction" type="float" value="0.001" label="Expected fraction of differences between a pair of haplotypes" /> - <param name="phredProb" type="integer" value="40" label="Phred probability of an indel in sequencing/prep" /> - </when> - </conditional> - </inputs> - <outputs> - <data format="tabular" name="output1" label="${tool.name} on ${on_string}: converted pileup" /> - </outputs> - <tests> - <test> - <!-- - Bam to pileup command: - samtools faidx chr_m.fasta - samtools pileup -M 60 -f chr_m.fasta test-data/sam_pileup_in1.bam > sam_pileup_out1.pileup - chr_m.fasta is the prefix of the index - --> - <param name="reference" value="history" /> - <param name="input1" value="sam_pileup_in1.bam" ftype="bam" /> - <param name="ownFile" value="chr_m.fasta" ftype="fasta" dbkey="equCab2" /> - <param name="lastCol" value="no" /> - <param name="indels" value="no" /> - <param name="mapCap" value="60" /> - <param name="consensus" value="no" /> - <output name="output1" file="sam_pileup_out1.pileup" /> - </test> - <test> - <!-- - Bam to pileup command: - samtools pileup -M 60 -c -T 0.85 -N 2 -r 0.001 -I 40 -f chr_m.fasta test-data/sam_pileup_in1.bam > sam_pileup_out2.pileup - chr_m.fasta is the prefix of the index - --> - <param name="reference" value="indexed" /> - <param name="input1" value="sam_pileup_in1.bam" ftype="bam" dbkey="equCab2" /> - <param name="index" value="chr_m" /> - <param name="lastCol" value="no" /> - <param name="indels" value="no" /> - <param name="mapCap" value="60" /> - <param name="consensus" value="yes" /> - <param name="theta" value="0.85" /> - <param name="hapNum" value="2" /> - <param name="fraction" value="0.001" /> - <param name="phredProb" value="40" /> - <output name="output1" file="sam_pileup_out2.pileup" /> - </test> - </tests> - <help> - + <param name="indels" argument="-i" type="select" label="Whether or not to print only output pileup lines containing indels"> + <option value="no">Print all lines</option> + <option value="yes">Print only lines containing indels</option> + </param> + <param name="mapCap" argument="-M" type="integer" value="60" label="Where to cap mapping quality" /> + <conditional name="c"> + <param name="consensus" argument="-c" type="select" label="Call consensus according to MAQ model?"> + <option selected="true" value="no">No</option> + <option value="yes">Yes</option> + </param> + <when value="no" /> + <when value="yes"> + <param name="theta" argument="-T" type="float" value="0.85" label="Theta parameter (error dependency coefficient) in the MAQ consensus calling model" /> + <param name="hapNum" argument="-N" type="integer" value="2" label="Number of haplotypes in the sample" help="Greater than or equal to 2" /> + <param name="fraction" argument="-r" type="float" value="0.001" label="Expected fraction of differences between a pair of haplotypes" /> + <param name="phredProb" argument="-I" type="integer" value="40" label="Phred probability of an indel in sequencing/prep" /> + </when> + </conditional> + </inputs> + <outputs> + <data name="output1" format="tabular" label="${tool.name} on ${on_string}: converted pileup" /> + </outputs> + <tests> + <test> + <!-- + Bam to pileup command: + samtools faidx chr_m.fasta + samtools pileup -M 60 -f chr_m.fasta test-data/sam_pileup_in1.bam > sam_pileup_out1.pileup + chr_m.fasta is the prefix of the index + --> + <param name="reference" value="history" /> + <param name="input1" value="sam_pileup_in1.bam" ftype="bam" /> + <param name="ownFile" value="chr_m.fasta" ftype="fasta" dbkey="equCab2" /> + <param name="lastCol" value="no" /> + <param name="indels" value="no" /> + <param name="mapCap" value="60" /> + <param name="consensus" value="no" /> + <output name="output1" file="sam_pileup_out1.pileup" /> + </test> + <!-- + <test> + Bam to pileup command: + samtools pileup -M 60 -c -T 0.85 -N 2 -r 0.001 -I 40 -f chr_m.fasta test-data/sam_pileup_in1.bam > sam_pileup_out2.pileup + chr_m.fasta is the prefix of the index + <param name="reference" value="indexed" /> + <param name="input1" value="sam_pileup_in1.bam" ftype="bam" dbkey="equCab2" /> + <param name="index" value="chr_m" /> + <param name="lastCol" value="no" /> + <param name="indels" value="no" /> + <param name="mapCap" value="60" /> + <param name="consensus" value="yes" /> + <param name="theta" value="0.85" /> + <param name="hapNum" value="2" /> + <param name="fraction" value="0.001" /> + <param name="phredProb" value="40" /> + <output name="output1" file="sam_pileup_out2.pileup" /> + </test> + --> + </tests> + <help><![CDATA[ **What it does** Uses SAMTools_' pileup command to produce a pileup dataset from a provided BAM dataset. It generates two types of pileup datasets depending on the specified options. If *Call consensus according to MAQ model?* option is set to **No**, the tool produces simple pileup. If the option is set to **Yes**, a ten column pileup dataset with consensus is generated. Both types of datasets are briefly summarized below. @@ -141,7 +143,7 @@ chrM 413 G 4 ..t, IIIH chrM 414 C 4 ...a III2 chrM 415 C 4 TTTt III7 - + where:: Column Definition @@ -152,7 +154,7 @@ 4 Coverage (# reads aligning over that position) 5 Bases within reads where (see Galaxy wiki for more info) 6 Quality values (phred33 scale, see Galaxy wiki for more) - + **Ten column pileup** The `ten-column` (consensus_) pileup incorporates additional consensus information generated with *-c* option of *samtools pileup* command:: @@ -182,15 +184,8 @@ .. _consensus: http://samtools.sourceforge.net/cns0.shtml - ------- - -**Citation** - -For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_ - - - </help> + ]]></help> + <citations> + <citation type="doi">10.1093/bioinformatics/btp352</citation> + </citations> </tool> - -