Mercurial > repos > devteam > samtools_bedcov
changeset 1:8c3472790020 draft
Uploaded
author | devteam |
---|---|
date | Tue, 21 Apr 2015 15:05:45 -0400 |
parents | c04bee391baa |
children | 12749212f61b |
files | macros.xml samtools_bedcov.xml |
diffstat | 2 files changed, 54 insertions(+), 5 deletions(-) [+] |
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--- a/macros.xml Wed Mar 18 16:43:50 2015 -0400 +++ b/macros.xml Tue Apr 21 15:05:45 2015 -0400 @@ -7,7 +7,37 @@ </xml> <xml name="citations"> <citations> + <citation type="bibtex"> + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + </citation> <citation type="doi">10.1093/bioinformatics/btp352</citation> + <citation type="doi">10.1093/bioinformatics/btr076</citation> + <citation type="doi">10.1093/bioinformatics/btr509</citation> + <citation type="bibtex"> + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + </citation> + <citation type="bibtex"> + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + </citation> + <citation type="bibtex"> + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + </citation> + <citation type="bibtex"> + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + </citation> </citations> </xml> <xml name="version_command"> @@ -18,4 +48,23 @@ <exit_code range="1:" level="fatal" description="Error" /> </stdio> </xml> + <token name="@no-chrom-options@"> +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + </token> + </macros>
--- a/samtools_bedcov.xml Wed Mar 18 16:43:50 2015 -0400 +++ b/samtools_bedcov.xml Tue Apr 21 15:05:45 2015 -0400 @@ -1,11 +1,11 @@ -<tool id="samtools_bedcov" name="Calculate read depth" version="1.0.1"> - <description>on BAM files</description> +<tool id="samtools_bedcov" name="BedCov" version="2.0"> + <description>calculate read depth for a set of genomic intervals</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"></expand> + <expand macro="stdio"></expand> <expand macro="version_command"></expand> - <expand macro="stdio"></expand> <command><![CDATA[ for bamfile in #for dataset in $input_bams: @@ -47,9 +47,9 @@ <help> **What it does** -This tool runs the ``samtools bedcov`` command in the SAMtools toolkit. +Calculates read depth for regions listed in a BED dataset using ``samtools bedcov`` command:: -Show read depth per BED region. + samtools bedcov [INPUT BED] [INPUT BAM1] ... [INPUT BAMn] > [OUTPUT] </help> <expand macro="citations"></expand>