view samtools_calmd.xml @ 1:33208952b99d draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_calmd commit 411130b45dc30f7f24f41cdeec5e148c5d8faf40

<tool id="samtools_calmd" name="CalMD" version="2.0.1">
    <description>recalculate MD/NM tags</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements"/>
    <expand macro="stdio"/>
    <expand macro="version_command"/>
    <command><![CDATA[
        #if str( $reference_source.reference_source_selector ) == "history":
            #set ref_fa = 'ref.fa'
            ln -s '${reference_source.ref_fasta}' ref.fa &&
            samtools faidx ref.fa &&
        #else:
            #set ref_fa = str( $reference_source.ref_fasta.fields.path )
        #end if

        samtools calmd
            #if str($option_set.option_sets) == 'advanced':
                $option_set.change_identical $option_set.modify_quality $option_set.compute_cap $option_set.extended_baq
            #end if
            -b
            '$input_bam'
            '$ref_fa'
            > '$calmd_output'
    ]]></command>
    <inputs>
        <param name="input_bam" type="data" format="bam" label="BAM file to recalculate" />
        <conditional name="reference_source">
            <param name="reference_source_selector" type="select" label="Choose the source for the reference genome">
                <option value="cached">Use a built-in genome</option>
                <option value="history">Use a genome from the history</option>
            </param>
            <when value="cached">
                <param name="ref_fasta" type="select" label="Using reference genome">
                    <options from_data_table="fasta_indexes">
                        <filter type="data_meta" column="1" key="dbkey" ref="input_bam" />
                        <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file" />
                    </options>
                </param>
            </when>
            <when value="history">
                <param name="ref_fasta" type="data" format="fasta" label="Using reference file" />
            </when>
        </conditional>
        <conditional name="option_set">
            <param name="option_sets" type="select" label="Options">
                <option value="default">Use defaults</option>
                <option value="advanced">Advanced options</option>
            </param>
            <when value="default" />
            <when value="advanced">
                <param name="change_identical" argument="-e" type="boolean" truevalue="-e" falsevalue="" checked="False" label="Change identical bases to '='" />
                <param name="modify_quality" argument="-A" type="boolean" truevalue="-A" falsevalue="" checked="False" label="Modify the quality string" />
                <param name="compute_cap" argument="-r" type="boolean" truevalue="-r" falsevalue="" checked="False" label="Compute BQ or cap baseQ by BAQ" />
                <param name="extended_baq" argument="-E" type="boolean" truevalue="-E" falsevalue="" checked="False" label="Extended BAQ for better sensitivity" />
            </when>
        </conditional>
    </inputs>
    <outputs>
        <data name="calmd_output" format="bam" label="${tool.name} on ${on_string}" />
    </outputs>
    <tests>
        <test>
            <param name="option_sets" value="default" />
            <param name="input_bam" value="phiX.bam"/>
            <param name="reference_source_selector" value="history" />
            <param name="ref_fasta" value="phiX.fasta" />
            <output name="calmd_output" file="samtools_calmd_out_1.bam" ftype="bam" />
        </test>
        <test>
            <param name="option_sets" value="advanced" />
            <param name="change_identical" value="true" />
            <param name="extended_baq" value="true" />
            <param name="input_bam" value="phiX.bam"/>
            <param name="reference_source_selector" value="history" />
            <param name="ref_fasta" value="phiX.fasta" />
            <output name="calmd_output" file="samtools_calmd_out_2.bam" ftype="bam" />
        </test>
    </tests>
    <help><![CDATA[
**What it does**

Generates the MD tag using the ``samtools calmd`` command. If the MD tag (see SAM format reference below for explanation of SAM/BAM tags) is already present, this command will give a warning if the MD tag generated is different from the existing tag. Outputs a BAM file.

-----

**NM and MD tags**

From the SAM format specification::

  MD (string)  String for mismatching positions. Regex : [0-9]+(([A-Z]|\^[A-Z]+)[0-9]+)*7
  NM (indeger) Edit distance to the reference, including ambiguous bases but excluding clipping

See references for more information about SAM format tags.
    ]]></help>
    <expand macro="citations"/>
</tool>