# HG changeset patch # User devteam # Date 1429646431 14400 # Node ID 87398ae795c7b49263b856bfa79e2b9faa5ade4f Uploaded diff -r 000000000000 -r 87398ae795c7 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Apr 21 16:00:31 2015 -0400 @@ -0,0 +1,70 @@ + + + + samtools + + + + + + + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + + 10.1093/bioinformatics/btp352 + 10.1093/bioinformatics/btr076 + 10.1093/bioinformatics/btr509 + + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + + + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + + + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + + + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + + + + + samtools --version | head -n 1 | awk '{ print $2 }' + + + + + + + +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + + + diff -r 000000000000 -r 87398ae795c7 samtools_idxstats.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/samtools_idxstats.xml Tue Apr 21 16:00:31 2015 -0400 @@ -0,0 +1,72 @@ + + tabulate mapping statistics for BAM dataset + + macros.xml + + + + + "${output}" ; +else + samtools idxstats "${input}" > "${output}" ; +fi]]> + + + + + + + + + + + + + + +`_ for the samtools idxstats command +]]> + + + diff -r 000000000000 -r 87398ae795c7 test-data/phiX.bam Binary file test-data/phiX.bam has changed diff -r 000000000000 -r 87398ae795c7 test-data/samtools_idxstats_out.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/samtools_idxstats_out.tabular Tue Apr 21 16:00:31 2015 -0400 @@ -0,0 +1,2 @@ +phiX174 5386 972 18 +* 0 0 10 diff -r 000000000000 -r 87398ae795c7 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Tue Apr 21 16:00:31 2015 -0400 @@ -0,0 +1,6 @@ + + + + + +