# HG changeset patch # User devteam # Date 1429649830 14400 # Node ID 020e144b5f78c1b3082eb645b1ff15b57b8a78b1 Uploaded diff -r 000000000000 -r 020e144b5f78 macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Tue Apr 21 16:57:10 2015 -0400 @@ -0,0 +1,70 @@ + + + + samtools + + + + + + + @misc{SAM_def, + title={Definition of SAM/BAM format}, + url = {https://samtools.github.io/hts-specs/SAMv1.pdf},} + + 10.1093/bioinformatics/btp352 + 10.1093/bioinformatics/btr076 + 10.1093/bioinformatics/btr509 + + @misc{Danecek_et_al, + Author={Danecek, P., Schiffels, S., Durbin, R.}, + title={Multiallelic calling model in bcftools (-m)}, + url = {http://samtools.github.io/bcftools/call-m.pdf},} + + + @misc{Durbin_VCQC, + Author={Durbin, R.}, + title={Segregation based metric for variant call QC}, + url = {http://samtools.github.io/bcftools/rd-SegBias.pdf},} + + + @misc{Li_SamMath, + Author={Li, H.}, + title={Mathematical Notes on SAMtools Algorithms}, + url = {http://www.broadinstitute.org/gatk/media/docs/Samtools.pdf},} + + + @misc{SamTools_github, + title={SAMTools GitHub page}, + url = {https://github.com/samtools/samtools},} + + + + + samtools --version | head -n 1 | awk '{ print $2 }' + + + + + + + +----- + +.. class:: warningmark + +**No options available? How to re-detect metadata** + +If you see a "No options available" within the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop down, you need to re-detect metadata for the dataset you are trying to process. To do this follow these steps: + +1. Click on the **pencil** icon adjacent to the dataset in the history +2. A new menu will appear in the center pane of the interface +3. Click **Datatype** tab +4. Set **New Type** to **BAM** +5. Click **Save** + +The medatada will be re-detected and you will be able to see the list of reference sequences in the "**Select references (chromosomes and contigs) you would like to restrict bam to**" drop-down. + + + + diff -r 000000000000 -r 020e144b5f78 samtools_reheader.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/samtools_reheader.xml Tue Apr 21 16:57:10 2015 -0400 @@ -0,0 +1,32 @@ + + copy SAM/BAM header between datasets + + macros.xml + + + + + "${output}"]]> + + + + + + + + + + + + + + + +**What it does** + +Copies header from *source* dataset into *target* dataset using ``samtools reheader`` command. + + + + + diff -r 000000000000 -r 020e144b5f78 test-data/reheader_input_file.bam Binary file test-data/reheader_input_file.bam has changed diff -r 000000000000 -r 020e144b5f78 test-data/reheader_input_header.bam Binary file test-data/reheader_input_header.bam has changed diff -r 000000000000 -r 020e144b5f78 test-data/reheader_out.bam Binary file test-data/reheader_out.bam has changed diff -r 000000000000 -r 020e144b5f78 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Tue Apr 21 16:57:10 2015 -0400 @@ -0,0 +1,6 @@ + + + + + +