Mercurial > repos > devteam > sicer
diff test-data/peakcalling_sicer/test_2/output_log_file.contains @ 0:82a8234e03f2 draft
Imported from capsule None
author | devteam |
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date | Mon, 27 Jan 2014 09:28:33 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/peakcalling_sicer/test_2/output_log_file.contains Mon Jan 27 09:28:33 2014 -0500 @@ -0,0 +1,287 @@ +############################################# +###### SICER v1.1 ###### +############################################# +Input library directory: +ChIP library: input_bed_file.bed +Control library: input_control_file.bed +Output directory: +Species: mm8 +Threshold for redundancy allowed for chip reads: 1 +Threshold for redundancy allowed for control reads: 1 +Window size: 200 bps +Fragment size: 150 bps. The shift for reads is half of 150 +Effective genome size as a fraction of the reference genome of mm8: 0.740000 +Gap size: 600 bps +Evalue for identification of candidate islands that exhibit clustering: 1000 +False discovery rate controlling significance: 0.01 +Preprocess the raw input_bed_file file to remove redundancy with threshold 1... +chr1 Plus reads: 4989 Retained plus reads: 4935 ; Minus reads: 5011 Retained minus reads: 4963 +chr2 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr3 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr4 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr5 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr6 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr7 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr8 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr9 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr10 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr11 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr12 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr13 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr14 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr15 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr16 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr17 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr18 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr19 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrX Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrY Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrM Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +Preprocess the raw input_control_file file to remove redundancy with threshold 1... +chr1 Plus reads: 4980 Retained plus reads: 4937 ; Minus reads: 5020 Retained minus reads: 4959 +chr2 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr3 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr4 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr5 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr6 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr7 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr8 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr9 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr10 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr11 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr12 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr13 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr14 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr15 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr16 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr17 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr18 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chr19 Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrX Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrY Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +chrM Plus reads: 0 Retained plus reads: 0 ; Minus reads: 0 Retained minus reads: 0 +Partion the genome in windows ... +Generate summary files ... +total tag count in chr1.bed is: 9898.0 = 4935.0+4963.0 +total tag count in chr2.bed is: 0.0 = 0.0+0.0 +total tag count in chr3.bed is: 0.0 = 0.0+0.0 +total tag count in chr4.bed is: 0.0 = 0.0+0.0 +total tag count in chr5.bed is: 0.0 = 0.0+0.0 +total tag count in chr6.bed is: 0.0 = 0.0+0.0 +total tag count in chr7.bed is: 0.0 = 0.0+0.0 +total tag count in chr8.bed is: 0.0 = 0.0+0.0 +total tag count in chr9.bed is: 0.0 = 0.0+0.0 +total tag count in chr10.bed is: 0.0 = 0.0+0.0 +total tag count in chr11.bed is: 0.0 = 0.0+0.0 +total tag count in chr12.bed is: 0.0 = 0.0+0.0 +total tag count in chr13.bed is: 0.0 = 0.0+0.0 +total tag count in chr14.bed is: 0.0 = 0.0+0.0 +total tag count in chr15.bed is: 0.0 = 0.0+0.0 +total tag count in chr16.bed is: 0.0 = 0.0+0.0 +total tag count in chr17.bed is: 0.0 = 0.0+0.0 +total tag count in chr18.bed is: 0.0 = 0.0+0.0 +total tag count in chr19.bed is: 0.0 = 0.0+0.0 +total tag count in chrX.bed is: 0.0 = 0.0+0.0 +total tag count in chrY.bed is: 0.0 = 0.0+0.0 +total tag count in chrM.bed is: 0.0 = 0.0+0.0 +Normalize summary graph by total island filtered reads per million for input_bed_file ... +Convert the normalized summary graph into wig vstep format... +Find candidate islands exhibiting clustering ... +Species: mm8 +Window_size: 200 +Gap size: 600 +E value is: 1000.0 +Total read count: 9898.0 +Genome Length: 2644093988 +Effective genome Length: 1956629551 +Window average: 0.0010117398048 +Window pvalue: 0.2 +Minimum num of tags in a qualified window: 1 +Generate the enriched probscore summary graph and filter the summary graph to get rid of ineligible windows +Determine the score threshold from random background +The score threshold is: 6.898 +Make and write islands +Total number of islands: 417 +Calculate significance of candidate islands using the control library ... +chip library size 9898.0 +control library size 9896.0 +Total number of chip reads on islands is: 9811 +Total number of control reads on islands is: 8514 +Identify significant islands using FDR criterion ... +Given significance 0.01 , there are 184 significant islands +Convert island summary to island bed file of format chr start end ChIP-read-count +Filter reads with identified significant islands... +Make summary graph with filtered reads... +total tag count in chr1.bed is: 4303.0 = 2148.0+2155.0 +total tag count in chr2.bed is: 0.0 = 0.0+0.0 +total tag count in chr3.bed is: 0.0 = 0.0+0.0 +total tag count in chr4.bed is: 0.0 = 0.0+0.0 +total tag count in chr5.bed is: 0.0 = 0.0+0.0 +total tag count in chr6.bed is: 0.0 = 0.0+0.0 +total tag count in chr7.bed is: 0.0 = 0.0+0.0 +total tag count in chr8.bed is: 0.0 = 0.0+0.0 +total tag count in chr9.bed is: 0.0 = 0.0+0.0 +total tag count in chr10.bed is: 0.0 = 0.0+0.0 +total tag count in chr11.bed is: 0.0 = 0.0+0.0 +total tag count in chr12.bed is: 0.0 = 0.0+0.0 +total tag count in chr13.bed is: 0.0 = 0.0+0.0 +total tag count in chr14.bed is: 0.0 = 0.0+0.0 +total tag count in chr15.bed is: 0.0 = 0.0+0.0 +total tag count in chr16.bed is: 0.0 = 0.0+0.0 +total tag count in chr17.bed is: 0.0 = 0.0+0.0 +total tag count in chr18.bed is: 0.0 = 0.0+0.0 +total tag count in chr19.bed is: 0.0 = 0.0+0.0 +total tag count in chrX.bed is: 0.0 = 0.0+0.0 +total tag count in chrY.bed is: 0.0 = 0.0+0.0 +total tag count in chrM.bed is: 0.0 = 0.0+0.0 +Normalize summary graph with filtered reads for input_bed_file by total island filtered reads per million... +Convert the summary graph made with the filtered reads into wig vstep format and normalize by total island-filtered read count per million... +Done! +Additionally, these warnings were reported: +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in +Warning: chr2 reads do not exist in +Warning: chr3 reads do not exist in +Warning: chr4 reads do not exist in +Warning: chr5 reads do not exist in +Warning: chr6 reads do not exist in +Warning: chr7 reads do not exist in +Warning: chr8 reads do not exist in +Warning: chr9 reads do not exist in +Warning: chr10 reads do not exist in +Warning: chr11 reads do not exist in +Warning: chr12 reads do not exist in +Warning: chr13 reads do not exist in +Warning: chr14 reads do not exist in +Warning: chr15 reads do not exist in +Warning: chr16 reads do not exist in +Warning: chr17 reads do not exist in +Warning: chr18 reads do not exist in +Warning: chr19 reads do not exist in +Warning: chrX reads do not exist in +Warning: chrY reads do not exist in +Warning: chrM reads do not exist in