comparison extractSplitReads_BwaMem.py @ 0:8b3daa745d9b draft

planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/lumpy commit c0bfc4b2215705e1b5fd1d4e60b1d72e5da13c92

0:8b3daa745d9b

#!/usr/bin/env python

import sys
import getopt
import string
from optparse import OptionParser
import re

def extractSplitsFromBwaMem(inFile,numSplits,includeDups,minNonOverlap):
    if inFile == "stdin":
        data = sys.stdin
    else:
        data = open(inFile, 'r')
    for line in data:
        split = 0
        if line[0] == '@':
            print line.strip()
            continue
        samList = line.strip().split('\t')
        sam = SAM(samList)
        if includeDups==0 and (1024 & sam.flag)==1024:
            continue
        for el in sam.tags:
            if "SA:" in el:
                if(len(el.split(";")))<=numSplits:
                    split = 1
                    mate = el.split(",")
                    mateCigar = mate[3]
                    mateFlag = int(0)
                    if mate[2]=="-": mateFlag = int(16)
        if split:
            read1 = sam.flag & 64
            if read1 == 64: tag = "_1"
            else: tag="_2"
            samList[0] = sam.query + tag
            readCigar = sam.cigar
            readCigarOps = extractCigarOps(readCigar,sam.flag)
            readQueryPos = calcQueryPosFromCigar(readCigarOps)
            mateCigarOps = extractCigarOps(mateCigar,mateFlag)
            mateQueryPos = calcQueryPosFromCigar(mateCigarOps)
            overlap = calcQueryOverlap(readQueryPos.qsPos,readQueryPos.qePos,mateQueryPos.qsPos,mateQueryPos.qePos)
            nonOverlap1 = 1 + readQueryPos.qePos - readQueryPos.qsPos - overlap
            nonOverlap2 = 1 + mateQueryPos.qePos - mateQueryPos.qsPos - overlap
            mno = min(nonOverlap1, nonOverlap2)
            if mno >= minNonOverlap:
                print "\t".join(samList)

#--------------------------------------------------------------------------------------------------
# functions
#--------------------------------------------------------------------------------------------------

class SAM (object):
    """
    __very__ basic class for SAM input.
    """
    def __init__(self, samList = []):
        if len(samList) > 0:
            self.query    = samList[0]
            self.flag     = int(samList[1])
            self.ref      = samList[2]
            self.pos      = int(samList[3])
            self.mapq     = int(samList[4])
            self.cigar    = samList[5]
            self.matRef   = samList[6]
            self.matePos  = int(samList[7])
            self.iSize    = int(samList[8])
            self.seq      = samList[9]
            self.qual     = samList[10]
            self.tags     = samList[11:]#tags is a list of each tag:vtype:value sets
            self.valid    = 1
        else:
            self.valid = 0
            self.query = 'null'

    def extractTagValue (self, tagID):
        for tag in self.tags:
            tagParts = tag.split(':', 2);
            if (tagParts[0] == tagID):
                if (tagParts[1] == 'i'):
                    return int(tagParts[2]);
                elif (tagParts[1] == 'H'):
                    return int(tagParts[2],16);
                return tagParts[2];
        return None;

#-----------------------------------------------
cigarPattern = '([0-9]+[MIDNSHP])'
cigarSearch = re.compile(cigarPattern)
atomicCigarPattern = '([0-9]+)([MIDNSHP])'
atomicCigarSearch = re.compile(atomicCigarPattern)

def extractCigarOps(cigar,flag):
    if (cigar == "*"):
        cigarOps = []
    elif (flag & 0x0010):
        cigarOpStrings = cigarSearch.findall(cigar)
        cigarOps = []
        for opString in cigarOpStrings:
            cigarOpList = atomicCigarSearch.findall(opString)
#            print cigarOpList
            # "struct" for the op and it's length
            cigar = cigarOp(cigarOpList[0][0], cigarOpList[0][1])
            # add to the list of cigarOps
            cigarOps.append(cigar)
            cigarOps = cigarOps
        cigarOps.reverse()
        ##do in reverse order because negative strand##
    else:
        cigarOpStrings = cigarSearch.findall(cigar)
        cigarOps = []
        for opString in cigarOpStrings:
            cigarOpList = atomicCigarSearch.findall(opString)
            # "struct" for the op and it's length
            cigar = cigarOp(cigarOpList[0][0], cigarOpList[0][1])
            # add to the list of cigarOps
            cigarOps.append(cigar)
#            cigarOps = cigarOps
    return(cigarOps)

def calcQueryPosFromCigar(cigarOps):
    qsPos = 0
    qePos = 0
    qLen  = 0
    # if first op is a H, need to shift start position
    # the opPosition counter sees if the for loop is looking at the first index of the cigar object
    opPosition = 0
    for cigar in cigarOps:
        if opPosition == 0 and (cigar.op == 'H' or cigar.op == 'S'):
            qsPos += cigar.length
            qePos += cigar.length
            qLen  += cigar.length
        elif opPosition > 0 and (cigar.op == 'H' or cigar.op == 'S'):
            qLen  += cigar.length
        elif cigar.op == 'M' or cigar.op == 'I':
            qePos += cigar.length
            qLen  += cigar.length
            opPosition += 1
    d = queryPos(qsPos, qePos, qLen);
    return d

class cigarOp (object):
    """
    sturct to store a discrete CIGAR operations
    """
    def __init__(self, opLength, op):
        self.length = int(opLength)
        self.op     = op

class queryPos (object):
    """
    struct to store the start and end positions of query CIGAR operations
    """
    def __init__(self, qsPos, qePos, qLen):
        self.qsPos = int(qsPos)
        self.qePos = int(qePos)
        self.qLen  = int(qLen)


def calcQueryOverlap(s1,e1,s2,e2):
    o = 1 + min(e1, e2) - max(s1, s2)
    return max(0, o)

###############################################

class Usage(Exception):
    def __init__(self, msg):
        self.msg = msg

def main():

    usage = """%prog -i <file>

extractSplitReads_BwaMem v0.1.0
Author: Ira Hall
Description: Get split-read alignments from bwa-mem in lumpy compatible format. Ignores reads marked as duplicates.
Works on read or position sorted SAM input. Tested on bwa mem v0.7.5a-r405.
    """
    parser = OptionParser(usage)

    parser.add_option("-i", "--inFile", dest="inFile",
        help="A SAM file or standard input (-i stdin).",
        metavar="FILE")
    parser.add_option("-n", "--numSplits", dest="numSplits", default=2, type = "int",
        help="The maximum number of split-read mappings to allow per read. Reads with more are excluded. Default=2",
        metavar="INT")
    parser.add_option("-d", "--includeDups", dest="includeDups", action="store_true",default=0,
        help="Include alignments marked as duplicates. Default=False")
    parser.add_option("-m", "--minNonOverlap", dest="minNonOverlap", default=20, type = "int",
        help="minimum non-overlap between split alignments on the query (default=20)",
        metavar="INT")
    (opts, args) = parser.parse_args()
    if opts.inFile is None:
        parser.print_help()
        print
    else:
        try:
            extractSplitsFromBwaMem(opts.inFile, opts.numSplits, opts.includeDups, opts.minNonOverlap)
        except IOError as err:
            sys.stderr.write("IOError " + str(err) + "\n");
            return
if __name__ == "__main__":
    sys.exit(main())