diff signature.py @ 0:a2f293717ce3 draft

planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/msp_sr_signature commit fe40dec87779c1fcfbd03330e653aa886f4a2cda

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/signature.py	Wed Oct 21 11:35:25 2015 -0400
@@ -0,0 +1,122 @@
+#!/usr/bin/python
+# script for computing overlap signatures from a bowtie output
+# Christophe Antoniewski <[e-mail]>
+# Usage signature.py <1:input> <2:format of input> <3:minsize query> <4:maxsize query> <5:minsize target> <6:maxsize target>
+#			  <7:minscope> <8:maxscope> <9:output> <10:bowtie index> <11:procedure option> <12: graph (global or lattice)>
+# 			  <13: R code>
+# version 2.0.0
+
+import sys
+import subprocess
+import argparse
+from smRtools import *
+from collections import defaultdict  # test whether it is required
+
+
+def Parser():
+    the_parser = argparse.ArgumentParser()
+    the_parser.add_argument(
+        '--input', action="store", type=str, help="input alignment file")
+    the_parser.add_argument('--inputFormat', action="store", type=str, choices=[
+                            "tabular", "bam", "sam"], help="format of alignment file (tabular/bam/sam)")
+    the_parser.add_argument(
+        '--minquery', type=int, help="Minimum readsize of query reads (nt) - must be an integer")
+    the_parser.add_argument(
+        '--maxquery', type=int, help="Maximum readsize of query reads (nt) - must be an integer")
+    the_parser.add_argument(
+        '--mintarget', type=int, help="Minimum readsize of target reads (nt) - must be an integer")
+    the_parser.add_argument(
+        '--maxtarget', type=int, help="Maximum readsize of target reads (nt) - must be an integer")
+    the_parser.add_argument(
+        '--minscope', type=int, help="Minimum overlap analyzed (nt) - must be an integer")
+    the_parser.add_argument(
+        '--maxscope', type=int, help="Maximum overlap analyzed (nt) - must be an integer")
+    the_parser.add_argument(
+        '--outputOverlapDataframe', action="store", type=str, help="Overlap dataframe")
+    the_parser.add_argument('--referenceGenome', action='store',
+                            help="path to the bowtie-indexed or fasta reference")
+    the_parser.add_argument('--extract_index', action='store_true',
+                            help="specify if the reference is an indexed Bowtie reference")
+    the_parser.add_argument('--graph', action='store', choices=[
+                            "global", "lattice"], help="small RNA signature is computed either globally or by item (global-lattice)")
+    the_parser.add_argument(
+        '--rcode', type=str, help="R code to be passed to the python script")
+    args = the_parser.parse_args()
+    return args
+
+args = Parser()
+
+if args.extract_index:
+    GenomeFormat = "bowtieIndex"
+else:
+    GenomeFormat = "fastaSource"
+
+if args.inputFormat == "tabular":
+    Genome = HandleSmRNAwindows(
+        args.input, args.inputFormat, args.referenceGenome, GenomeFormat)
+elif args.inputFormat == "sam":
+    Genome = HandleSmRNAwindows(
+        args.input, args.inputFormat, args.referenceGenome, GenomeFormat)
+else:
+    Genome = HandleSmRNAwindows(
+        args.input, args.inputFormat, args.referenceGenome, GenomeFormat)
+
+# replace objDic by Genome.instanceDict or... objDic = Genome.instanceDict
+objDic = Genome.instanceDict
+
+args.maxscope += 1
+
+general_frequency_table = dict(
+    [(i, 0) for i in range(args.minscope, args.maxscope)])
+general_percent_table = dict(
+    [(i, 0) for i in range(args.minscope, args.maxscope)])
+OUT = open(args.outputOverlapDataframe, "w")
+
+if args.graph == "global":
+    # for normalized summing of local_percent_table(s)
+    readcount_dic = {}
+    Total_read_in_objDic = 0
+    for item in objDic:
+        readcount_dic[item] = objDic[item].readcount(
+            args.minquery, args.maxquery)
+        Total_read_in_objDic += readcount_dic[item]
+    ######
+    for x in (objDic):
+        local_frequency_table = objDic[x].signature(
+            args.minquery, args.maxquery, args.mintarget, args.maxtarget, range(args.minscope, args.maxscope))
+        local_percent_table = objDic[x].hannon_signature(
+            args.minquery, args.maxquery, args.mintarget, args.maxtarget, range(args.minscope, args.maxscope))
+        try:
+            for overlap in local_frequency_table.keys():
+                general_frequency_table[overlap] = general_frequency_table.get(
+                    overlap, 0) + local_frequency_table[overlap]
+        except:
+            pass
+        try:
+            for overlap in local_percent_table.keys():
+                general_percent_table[overlap] = general_percent_table.get(
+                    overlap, 0) + (1. / Total_read_in_objDic * readcount_dic[x] * local_percent_table[overlap])
+        except:
+            pass
+    print >> OUT, "overlap\tnum of pairs\tprobability"
+    for classe in sorted(general_frequency_table):
+        print >> OUT, "%i\t%i\t%f" % (
+            classe, general_frequency_table[classe], general_percent_table[classe])
+
+else:
+    print >> OUT, "overlap\tnum of pairs\tprobability\titem"
+    for x in (objDic):
+        local_frequency_table = objDic[x].signature(
+            args.minquery, args.maxquery, args.mintarget, args.maxtarget, range(args.minscope, args.maxscope))
+        local_percent_table = objDic[x].hannon_signature(
+            args.minquery, args.maxquery, args.mintarget, args.maxtarget, range(args.minscope, args.maxscope))
+        for classe in range(args.minscope, args.maxscope):
+            print >> OUT, "%i\t%i\t%f\t%s" % (
+                classe, local_frequency_table[classe], local_percent_table[classe], x)
+
+OUT.close()
+
+# Run the R script that is defined in the xml using the Rscript binary
+# provided with R.
+R_command = "Rscript " + args.rcode
+process = subprocess.Popen(R_command.split())