annotate ete_species_tree_generator.py @ 1:a4ba317fc713 draft

planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
author earlhaminst
date Fri, 17 Mar 2017 16:23:39 -0400
parents
children 03c10736e497
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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1 import optparse
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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3 from ete3 import NCBITaxa
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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5 ncbi = NCBITaxa()
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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7 parser = optparse.OptionParser()
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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8 parser.add_option('-s', '--species', dest="input_species_filename",
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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9 help='Species list in text format one species in each line')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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11 parser.add_option('-f', '--format', type='choice', choices=['0', '1', '2', '3', '4', '5', '6', '7', '8', '9', '100'], dest="format",
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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12 default='8', help='outpur format for tree')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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14 parser.add_option('-t', '--treebest', type='choice', choices=['yes', 'no'], dest="treebest",
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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15 default='no', help='To be used in TreeBest')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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17 parser.add_option('-d', '--database', type='choice', choices=['yes', 'no'], dest="database",
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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18 default='no', help='Update database')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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20 options, args = parser.parse_args()
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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22 if options.database == "yes":
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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23 try:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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24 ncbi.update_taxonomy_database()
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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25 except:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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26 pass
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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28 if options.input_species_filename is None:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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29 raise Exception('-s option must be specified, Species list in text format one species in each line')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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31 with open(options.input_species_filename) as f:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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32 species_name = [_.strip().replace('_', ' ') for _ in f.readlines()]
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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34 name2taxid = ncbi.get_name_translator(species_name)
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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36 taxid = [name2taxid[_][0] for _ in species_name]
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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38 tree = ncbi.get_topology(taxid)
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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39
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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40 if options.treebest == "yes":
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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41 inv_map = {str(v[0]): k.replace(" ", "") + "*" for k, v in name2taxid.items()}
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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42 else:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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43 inv_map = {str(v[0]): k for k, v in name2taxid.items()}
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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45
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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46 for leaf in tree:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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47 leaf.name = inv_map[leaf.name]
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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49 newickTree = tree.write(format=int(options.format))
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
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50
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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51 if options.treebest == "yes":
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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52 newickTree = newickTree.rstrip(';')
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
parents:
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53 newickTree = newickTree + "root;"
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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54
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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55 with open('newickTree.nhx', 'w') as newickFile:
a4ba317fc713 planemo upload for repository https://github.com/TGAC/earlham-galaxytools/tree/master/tools/ete commit df73a2add6dba8550867034e157ed0699b3b2f53
earlhaminst
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56 newickFile.write(newickTree)