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author | earlhaminst |
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date | Wed, 12 Jul 2017 14:29:51 -0400 |
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<tool id="plotheatmap" name="plotHeatmap" version="1.0"> <description>Plot heatmap</description> <requirements> <requirement type="package" version="1.34.0">bioconductor-preprocesscore</requirement> <requirement type="package" version="3.0.1">r-gplots</requirement> </requirements> <command detect_errors="aggressive"><![CDATA[ Rscript '$__tool_directory__/script.R' '$count_matrix' '$stats_matrix' $filter.log_fc $filter.log_cpm $filter.pvalue $filter.fdr $heatmap.cluster_row $heatmap.cluster_col $heatmap.hclust_method $plotparm.col_marg $plotparm.row_marg $plotparm.pdf_width $plotparm.pdf_height ]]></command> <inputs> <param name="count_matrix" type="data" format="tabular" label="Output dataset 'count_matrix' from tool countMatrixForEdger"/> <param name="stats_matrix" type="data" format="tabular" label="Output dataset 'output' from tool edgeR"/> <section name="filter" title="Filtering parameters" expanded="true"> <param name="log_fc" type="float" value="0.5" label="A log FC threshold"/> <param name="log_cpm" type="float" value="0.5" label="A log CPM threshold"/> <param name="pvalue" type="float" min="0" max="1" value="0.5" label="A p-value threshold"/> <param name="fdr" type="float" min="0" max="1" value="0.5" label="An FDR threshold"/> </section> <section name="heatmap" title="Heatmap parameters"> <param name="cluster_row" type="boolean" truevalue="Yes" falsevalue="No" checked="true" label="Determines if the row dendrogram should be reordered"/> <param name="cluster_col" type="boolean" truevalue="Yes" falsevalue="No" label="Determines if the column dendrogram should be reordered"/> <param name="hclust_method" type="select" label="Hierarchical clustering method"> <option value="ward.D">ward.D</option> <option value="ward.D2">ward.D2</option> <option value="single">single</option> <option value="complete">complete</option> <option value="average" selected="true">average</option> <option value="mcquitty">mcquitty</option> <option value="median">median</option> <option value="centroid">centroid</option> </param> </section> <section name="plotparm" title="Plot parameters"> <param name="col_marg" type="float" value="7" label="Margins for column names"/> <param name="row_marg" type="float" value="8" label="Margins for row names"/> <param name="pdf_width" type="float" value="7" label="Width of the graphics region in inches (pdf width)"/> <param name="pdf_height" type="float" value="7" label="Height of the graphics region in inches (pdf height)"/> </section> </inputs> <outputs> <data name="haetamappdf" format="pdf" from_work_dir="heatmap.pdf"/> </outputs> <tests> <test> <param name="count_matrix" value="count.matrix"/> <param name="stats_matrix" value="stats.data"/> <param name="log_fc" value="1"/> <param name="log_cpm" value="2"/> <param name="pvalue" value="0.05"/> <param name="fdr" value="0.1"/> <output name="haetamappdf" file="heatmap.pdf" ftype="pdf" compare="sim_size"/> </test> </tests> <help><![CDATA[ **What it does** It takes a list of genes based on set filtering criteria and draws them on heatmap. **Example** The following filtering options: A log FC threshold = 0.5 A log CPM threshold = 0.5 A p-value threshold = 0.05 An FDR threshold = 0.1 mean that you select genes whose values in output table from edgeR are: * absolute value of log FC is greater than or equal to 0.5 * log CPM is greater than or equal to 0.5 * p-value is less than or equal to 0.05 * FDR is less than or equal to 0.1 ]]></help> <citations> </citations> </tool>