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author | engineson |
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date | Mon, 24 Jul 2017 08:42:27 -0400 |
parents | 8f25feb85166 |
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##FastQC 0.11.4 >>Basic Statistics pass #Measure Value Filename poulet5_2 File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267849 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.590586487162543 21.0 21.0 21.0 20.0 21.0 2 20.458478471078855 21.0 19.0 22.0 19.0 22.0 3 20.178608096352797 21.0 20.0 21.0 18.0 22.0 4 22.495204387546714 23.0 22.0 24.0 20.0 24.0 5 24.59397272343746 25.0 24.0 26.0 24.0 26.0 6 25.77560491172265 26.0 25.0 27.0 25.0 27.0 7 26.651378201897337 27.0 26.0 28.0 26.0 28.0 8 27.54968284369178 28.0 27.0 29.0 26.0 29.0 9 27.089005372429988 28.0 26.0 28.0 26.0 28.0 10-11 27.058984726469017 28.0 26.0 28.0 25.5 28.0 12-13 26.954911162632676 27.5 26.0 28.0 25.5 28.0 14-15 27.066410552214123 28.0 26.0 28.0 26.0 28.0 16-17 27.04552938409328 28.0 26.0 28.0 25.5 28.0 18-19 27.025118630273028 28.0 26.0 28.0 25.0 28.0 20-21 27.019042445557012 28.0 26.0 28.0 25.0 28.0 22-23 27.010315513591614 28.0 26.0 28.0 25.0 28.0 24-25 27.03931879529138 28.0 26.0 28.0 25.5 28.0 26-27 26.978450544896567 28.0 26.0 28.0 25.0 28.0 28-29 27.003714779595967 28.0 26.0 28.0 25.0 28.0 30-31 27.025971722873706 28.0 26.0 28.0 25.0 28.0 32-33 27.017791740868923 28.0 26.0 28.0 25.0 28.0 34-35 27.00628899118533 28.0 26.0 28.0 25.0 28.0 36-37 27.00906294218011 28.0 26.0 28.0 25.0 28.0 38-39 27.034922661648913 28.0 26.0 28.0 25.5 28.0 40-41 27.005346295860726 28.0 26.0 28.0 25.0 28.0 42-43 27.000909094303132 28.0 26.0 28.0 25.0 28.0 44-45 27.004913216028434 28.0 26.0 28.0 25.0 28.0 46-47 26.979999925331065 28.0 26.0 28.0 25.0 28.0 48-49 26.941037674211962 27.5 26.0 28.0 25.0 28.0 50-51 26.99995146519121 27.5 26.0 28.0 25.5 28.0 52-53 26.95084543903468 27.5 26.0 28.0 25.0 28.0 54-55 26.97609847339359 28.0 26.0 28.0 25.0 28.0 56-57 26.99262270906369 27.5 26.0 28.0 25.0 28.0 58-59 26.942271578389317 27.0 26.0 28.0 25.0 28.0 60-61 26.88024222603034 27.0 26.0 28.0 25.0 28.0 62-63 26.866450873439888 27.0 26.0 28.0 25.0 28.0 64-65 26.947610407356386 28.0 26.0 28.0 25.0 28.0 66-67 26.975012040366025 28.0 26.5 28.0 25.0 28.5 68-69 26.966286975124042 28.0 26.5 28.0 25.0 29.0 70-71 26.917634562757375 28.0 26.0 28.0 25.0 29.0 72-73 26.885127441207544 28.0 26.0 28.0 25.0 29.0 74-75 26.846286527110426 28.0 26.5 28.0 25.0 29.0 76-77 26.769134848366058 28.0 26.0 28.0 25.0 29.0 78-79 26.709703975000842 28.0 26.5 28.0 25.0 29.0 80-81 26.551336760637525 27.5 26.0 28.0 25.0 28.5 82-83 26.42626069165836 27.5 26.0 28.0 25.0 28.5 84-85 26.309463541024982 27.5 26.0 28.0 25.0 29.0 86-87 26.127226534353312 27.5 26.0 28.0 25.0 29.0 88-89 25.91885539987082 27.5 26.0 28.0 24.0 29.0 90-91 25.674260497519125 27.0 26.0 28.0 23.5 29.0 92-93 25.371903572535274 27.0 26.0 28.0 22.0 29.0 94-95 24.758753999454918 27.0 26.0 28.0 20.5 28.5 96-97 23.84710415196622 26.5 25.0 27.5 7.0 28.0 98-99 22.40618968149965 25.5 24.0 26.5 2.0 27.0 100-101 23.009550156991438 27.0 25.0 28.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1408369887985721 1101 2 -0.019030947201084558 1101 3 -0.060059442415557385 1101 4 0.004085939732711097 1101 5 -0.018429270835582656 1101 6 -0.10404524639907464 1101 7 0.05541229462318498 1101 8 0.06703529503928962 1101 9 0.0644374620344621 1101 10-11 0.06977859906234585 1101 12-13 0.048103685880093394 1101 14-15 0.0848396420659725 1101 16-17 0.030233298636137107 1101 18-19 0.05253119669850648 1101 20-21 0.08961672433820667 1101 22-23 0.014190195653434046 1101 24-25 -0.013817302849609803 1101 26-27 0.046003756870838686 1101 28-29 0.04599618351544166 1101 30-31 0.04851104267937956 1101 32-33 0.04656194043190354 1101 34-35 0.057517795385400916 1101 36-37 -0.00326392946157128 1101 38-39 0.0753296536157606 1101 40-41 0.005995075411309614 1101 42-43 -0.013913414669129764 1101 44-45 -0.03438241660346364 1101 46-47 -0.028176852907705552 1101 48-49 0.024338955929199102 1101 50-51 0.028402595301869837 1101 52-53 -0.014925605805846232 1101 54-55 -0.039593664804112194 1101 56-57 -0.02319294555917395 1101 58-59 -0.05831481380350212 1101 60-61 -0.007231705782142939 1101 62-63 0.1346543703300931 1101 64-65 0.019757339374642413 1101 66-67 0.032473131921936016 1101 68-69 0.06580901615117085 1101 70-71 -5.192806625906599E-4 1101 72-73 0.007598890765713406 1101 74-75 0.018862762851210135 1101 76-77 0.032333821260792206 1101 78-79 0.012622327563921232 1101 80-81 0.07758176267470418 1101 82-83 0.0989714754431752 1101 84-85 0.0737115932200112 1101 86-87 0.1197152444492886 1101 88-89 0.2945766871252822 1101 90-91 0.39366183935213783 1101 92-93 0.38892187749654283 1101 94-95 0.4587836546800368 1101 96-97 0.2815282150812948 1101 98-99 0.27882391282161123 1101 100-101 0.2642351714779956 1102 1 -0.07975744334402535 1102 2 -0.03332450286213273 1102 3 -0.06284472012740139 1102 4 0.0368379869674591 1102 5 0.022244684777994905 1102 6 0.04829943079057486 1102 7 0.0964534173385907 1102 8 0.12806291787337543 1102 9 0.017503121896794482 1102 10-11 0.09683028432225882 1102 12-13 0.0801659040144358 1102 14-15 0.09997323155090498 1102 16-17 0.06881366001757883 1102 18-19 0.10386227016159566 1102 20-21 0.034994127613781956 1102 22-23 0.08425050006833601 1102 24-25 0.08546060185013005 1102 26-27 0.1378511637068236 1102 28-29 0.10893752578935434 1102 30-31 0.10323748464472615 1102 32-33 0.009366800389180696 1102 34-35 0.06916329740296234 1102 36-37 0.07905938527851575 1102 38-39 0.0742726574135304 1102 40-41 0.05207269231138412 1102 42-43 0.08908437491074395 1102 44-45 0.14235105716819518 1102 46-47 0.04198358798952029 1102 48-49 0.10279588982428223 1102 50-51 0.12128845454706294 1102 52-53 0.10804941199619833 1102 54-55 0.0783485675718687 1102 56-57 0.06077705800123567 1102 58-59 0.12424141097693919 1102 60-61 0.18279210444809735 1102 62-63 0.061083725896200036 1102 64-65 0.1318323156385901 1102 66-67 0.1546303684517234 1102 68-69 0.06580901615117085 1102 70-71 -0.014914827185258162 1102 72-73 0.020401525467825365 1102 74-75 0.10567182112322726 1102 76-77 0.3096377021057961 1102 78-79 0.12471399324158483 1102 80-81 0.06436931014681235 1102 82-83 0.158391351422285 1102 84-85 0.09494238185043713 1102 86-87 0.14155983262873306 1102 88-89 0.19229839671464788 1102 90-91 0.0736534575576897 1102 92-93 0.2624179758824887 1102 94-95 0.3205025901179681 1102 96-97 0.09446955735520746 1102 98-99 0.10838605678078395 1102 100-101 0.18711339068046584 1103 1 -0.027478622968690303 1103 2 -0.1440385635793433 1103 3 -0.14964709169656842 1103 4 -0.13658475853684848 1103 5 0.09513473720731369 1103 6 -0.05173976869983221 1103 7 0.009584546633909952 1103 8 -0.03515746026171129 1103 9 0.008483739148768876 1103 10-11 -0.0241998922854485 1103 12-13 0.004138142375339271 1103 14-15 -0.04413039510195205 1103 16-17 -0.0629473770889426 1103 18-19 0.019893111760655557 1103 20-21 -0.08973375092379854 1103 22-23 -0.06299608572604498 1103 24-25 -0.08417169293003823 1103 26-27 -0.04350772862757779 1103 28-29 0.02177990883837566 1103 30-31 0.06781094518973774 1103 32-33 0.013662994538655937 1103 34-35 -0.0994557605492794 1103 36-37 0.0023683822615581107 1103 38-39 -0.05731338196795832 1103 40-41 -0.057647381327658564 1103 42-43 -0.032320296828523 1103 44-45 -0.09455561304509175 1103 46-47 -0.03751262855966431 1103 48-49 -0.0791999942292243 1103 50-51 0.03275535547734876 1103 52-53 -0.01692871328817347 1103 54-55 -0.03720593021965968 1103 56-57 -0.04008988072855857 1103 58-59 0.0057732010636684095 1103 60-61 -0.042139471441416276 1103 62-63 -0.05063307178543042 1103 64-65 -0.05497075592148093 1103 66-67 -0.10105530763448556 1103 68-69 -0.18955291414373576 1103 70-71 -0.264748579346481 1103 72-73 -0.2096049844475445 1103 74-75 -0.06053226822360713 1103 76-77 -0.03143811390859241 1103 78-79 -0.024993060566711023 1103 80-81 -0.2590754850708521 1103 82-83 -0.15213861168819065 1103 84-85 -0.20318374250994253 1103 86-87 -0.18613565732989557 1103 88-89 -0.09352538757615747 1103 90-91 -0.265343385483348 1103 92-93 -0.3298985740023639 1103 94-95 -0.2816768315495537 1103 96-97 -0.2808918129469795 1103 98-99 -0.23923642412696466 1103 100-101 -0.4768822273765032 1104 1 0.05302527584276007 1104 2 0.08125278272845193 1104 3 0.02056353609894046 1104 4 -0.04722549898740169 1104 5 0.007003543649805266 1104 6 0.01756335068998638 1104 7 -0.0966286837757977 1104 8 -0.047158782008185796 1104 9 -0.0732022370529819 1104 10-11 -0.030071955402249273 1104 12-13 0.05307256944252714 1104 14-15 -0.06225501399270428 1104 16-17 -0.00528618704019479 1104 18-19 -0.027016965193606524 1104 20-21 -0.059669980062857064 1104 22-23 0.02991536747970258 1104 24-25 -0.0010413339804884458 1104 26-27 -0.03980649783633439 1104 28-29 -0.0640033111131828 1104 30-31 0.012131523215362705 1104 32-33 0.03574518809275773 1104 34-35 0.009702443646922632 1104 36-37 0.04744828319870109 1104 38-39 0.020329003852562977 1104 40-41 -0.025698929440252982 1104 42-43 0.09031852913357241 1104 44-45 1.579120323214056E-4 1104 46-47 -0.0071976236712458785 1104 48-49 -0.02969156128230921 1104 50-51 0.06718804349408458 1104 52-53 0.03710959692027416 1104 54-55 0.03789601118063857 1104 56-57 0.0724467824066366 1104 58-59 0.04586930020827751 1104 60-61 0.06556072375464694 1104 62-63 0.11500083850613407 1104 64-65 0.05466947695117241 1104 66-67 0.12048626435811016 1104 68-69 0.09058665274583433 1104 70-71 0.14136824532793923 1104 72-73 0.12740800477749303 1104 74-75 0.14069426481494318 1104 76-77 0.24723577921389506 1104 78-79 0.23572483853770265 1104 80-81 0.20312954914807335 1104 82-83 0.30653381661216983 1104 84-85 0.2658727417125597 1104 86-87 0.392778228161891 1104 88-89 0.5330045850691434 1104 90-91 0.6952052074357695 1104 92-93 0.659224270069025 1104 94-95 0.7095904439834904 1104 96-97 0.5908425705162017 1104 98-99 0.5160641880324199 1104 100-101 0.6854396519562194 1105 1 -0.028459544703729023 1105 2 0.11491317573407756 1105 3 -0.06757608754422151 1105 4 0.022193662510549217 1105 5 -0.09717231780715352 1105 6 -0.0075684653000749336 1105 7 -0.14541434320128843 1105 8 -0.013421427626180815 1105 9 -0.07258811668224752 1105 10-11 -0.05261928272212657 1105 12-13 -0.10918400093865088 1105 14-15 -0.11637064139284092 1105 16-17 -0.05324254836914477 1105 18-19 -0.09285820800988986 1105 20-21 -0.07322460614836501 1105 22-23 0.021047532151918347 1105 24-25 -0.07990004388792826 1105 26-27 -0.08927302984676899 1105 28-29 -0.08677681064120435 1105 30-31 -0.09086557267066198 1105 32-33 0.00996548296477684 1105 34-35 -0.06826178659010296 1105 36-37 -0.014692530989833585 1105 38-39 -0.05678315400307099 1105 40-41 -0.10301998246035637 1105 42-43 -0.027298872304818644 1105 44-45 -0.03805733794293786 1105 46-47 -0.07843794263688864 1105 48-49 0.040902439185739325 1105 50-51 -0.0378917214752974 1105 52-53 -0.09494513047153674 1105 54-55 0.010719221475245178 1105 56-57 -0.03464261687055625 1105 58-59 -0.05921203540143338 1105 60-61 0.06217128191310195 1105 62-63 0.036309401794301976 1105 64-65 -0.012835859299364927 1105 66-67 0.11030930031164132 1105 68-69 -0.011446855295059066 1105 70-71 0.05196818281645221 1105 72-73 0.0674440980923805 1105 74-75 -0.009656066511634975 1105 76-77 0.05342818579613606 1105 78-79 -0.18503242999310743 1105 80-81 -0.2726849456633147 1105 82-83 -0.32231444853704616 1105 84-85 -0.2874253673935385 1105 86-87 -0.34649233505402677 1105 88-89 -0.30932982624700855 1105 90-91 -0.21300657142435142 1105 92-93 -0.16528101749165813 1105 94-95 -0.08319262074417111 1105 96-97 -0.018117461399207002 1105 98-99 0.07278864902792392 1105 100-101 0.20900161293209152 1106 1 0.07103620744962669 1106 2 -0.19972178414192143 1106 3 -0.10142011547265639 1106 4 0.037252426024195984 1106 5 0.034965003540833095 1106 6 -0.002664732072133802 1106 7 -0.022748136215817283 1106 8 -0.04288727247256574 1106 9 -0.02577911702140412 1106 10-11 -0.017258867439405634 1106 12-13 -0.02078997006713479 1106 14-15 0.027646215452904244 1106 16-17 -0.01655634525171834 1106 18-19 -0.034552974091024424 1106 20-21 0.003783906362532008 1106 22-23 -0.07673983166052523 1106 24-25 -0.10074686634167307 1106 26-27 -0.06543267376202166 1106 28-29 -0.016784042211213546 1106 30-31 -0.07523921499945274 1106 32-33 -0.01794473344977021 1106 34-35 -0.010676906737874248 1106 36-37 -0.009232415340772349 1106 38-39 -0.06977895801070844 1106 40-41 -0.049019354603117904 1106 42-43 -0.05060526944134125 1106 44-45 -0.014737626869386844 1106 46-47 -0.10499574334266626 1106 48-49 -0.046569426363113564 1106 50-51 -0.06151765310052326 1106 52-53 -0.08565200242509619 1106 54-55 -0.106026487933363 1106 56-57 -0.15438045844311432 1106 58-59 -0.0815542232036286 1106 60-61 -0.19528732575740548 1106 62-63 -0.09971418769917761 1106 64-65 -0.09440404487602905 1106 66-67 -0.09661590899746031 1106 68-69 -0.1694517548238892 1106 70-71 -0.10756711873721514 1106 72-73 -0.19672753677500054 1106 74-75 -0.1254712017952535 1106 76-77 -0.2046856077229222 1106 78-79 -0.27057797907388803 1106 80-81 -0.15039152891466046 1106 82-83 -0.1826505483663503 1106 84-85 -0.2169259686229097 1106 86-87 -0.11096528533838423 1106 88-89 -0.19976243109776703 1106 90-91 -0.2882835713879146 1106 92-93 -0.3259774345009845 1106 94-95 -0.2986471585543491 1106 96-97 -0.17637672719865094 1106 98-99 -0.23470583619921115 1106 100-101 -0.2894441601031197 1107 1 -0.009937623524205463 1107 2 0.16342707900694364 1107 3 0.07375761327649855 1107 4 0.12149738526915499 1107 5 0.08925653997522787 1107 6 0.030437247458419137 1107 7 0.08891454687543643 1107 8 -0.09124941012041532 1107 9 -0.03751534304334214 1107 10-11 -0.0034963804626322315 1107 12-13 -0.06429137963997533 1107 14-15 0.011750217414050468 1107 16-17 0.01649583958618095 1107 18-19 -0.04077731067250312 1107 20-21 2.944475159303295E-4 1107 22-23 -0.07018608403534898 1107 24-25 0.05170916111435275 1107 26-27 -0.01075044050836027 1107 28-29 0.04910582725008439 1107 30-31 -0.09517241350407701 1107 32-33 -0.0022325927376272148 1107 34-35 -0.06758457971697496 1107 36-37 -0.057100436423080936 1107 38-39 -0.06114915473804672 1107 40-41 0.028978285894524447 1107 42-43 -0.01878263047584383 1107 44-45 -0.024746360092407826 1107 46-47 -0.08098321040156264 1107 48-49 -0.01709096831322654 1107 50-51 -0.010999022224748245 1107 52-53 -0.047045794176032274 1107 54-55 0.045288091952645715 1107 56-57 0.022325584334357274 1107 58-59 0.08893897407528684 1107 60-61 0.03996961485667683 1107 62-63 -0.05464999084926703 1107 64-65 0.07774003798234475 1107 66-67 -0.023046165784865735 1107 68-69 0.05623694407909596 1107 70-71 -0.010980736436543737 1107 72-73 -0.03616646549964386 1107 74-75 0.10632309249904282 1107 76-77 -0.07544156776459232 1107 78-79 0.031197010201104547 1107 80-81 -0.040283972378531985 1107 82-83 0.02021302949723136 1107 84-85 0.0776594331053495 1107 86-87 0.0016511576352016277 1107 88-89 0.07030483004092503 1107 90-91 0.08704200929480521 1107 92-93 0.06258204334420725 1107 94-95 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100-101 0.5558034486475201 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 308.0 3 0.0 4 0.0 5 2.0 6 2.0 7 5.0 8 11.0 9 19.0 10 24.0 11 37.0 12 67.0 13 75.0 14 135.0 15 215.0 16 364.0 17 551.0 18 904.0 19 1284.0 20 1843.0 21 2574.0 22 3707.0 23 5673.0 24 9154.0 25 18567.0 26 152994.0 27 69334.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.259742395586485 12.979270544437881 10.183447832489852 52.57753922748578 2 18.617901052497956 21.439745518763136 40.641206097693015 19.30114733104589 3 24.37754107724875 22.73930460819342 24.34692681324179 28.53622750131604 4 24.41562223491594 30.088221348595663 20.10050438866675 25.395652027821647 5 26.98610037745147 30.530261453281515 22.40926790841108 20.074370260855932 6 21.71820690015643 32.48808097099485 23.803336954776757 21.990375174071957 7 20.013888422208034 18.00865412975221 38.0975848332456 23.879872614794156 8 20.609746536294704 21.85895784565184 29.885495185720313 27.645800432333147 9 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26.149621615163767 26.046018465628023 23.88696616377138 34-35 24.097159220306963 26.049191895433623 25.611445254602405 24.24220362965701 36-37 23.718027694708585 25.954922362973164 25.862706226269278 24.464343716048965 38-39 23.9228072533405 25.979376439710432 26.10724699364194 23.990569313307127 40-41 23.882672699916743 25.983296558882056 26.044338414554467 24.08969232664673 42-43 23.787283133407257 26.000097069617585 25.91497448189092 24.297645315084246 44-45 23.768055882232154 26.142154721503534 26.11620726603422 23.973582130230092 46-47 23.956034930128542 26.0038305164477 25.79867761313277 24.241456940290984 48-49 23.8716590317679 25.95436234594865 25.834892047384905 24.339086574898545 50-51 23.940727798125064 26.11900735115681 25.965562686439004 23.974702164279126 52-53 23.85952532957002 26.227277309230203 25.698807910427146 24.214389450772636 54-55 23.936621006611936 25.95342898424112 25.899480677545935 24.210469331601015 56-57 24.102199373527622 25.939801903311192 26.084286295636723 23.873712427524463 58-59 24.02267695604613 26.216823658105877 25.769370055516355 23.991129330331642 60-61 23.884726095673308 26.040978312407365 25.961455894925873 24.112839696993454 62-63 23.794563354725984 26.08801974246684 26.275252100997204 23.842164801809975 64-65 23.963688496130285 26.01055072074191 25.83302532396985 24.19273545915796 66-67 23.792323286627916 25.963135945999426 25.901534073302496 24.343006694070166 68-69 23.88509944035632 26.06225895933903 26.086713036076297 23.965928564228353 70-71 23.78392303126015 26.232877479475373 25.739315808533913 24.243883680730562 72-73 23.80445698882579 26.11751397242476 25.980869818442482 24.097159220306963 74-75 23.956781619494567 26.278238858461293 26.054418720995788 23.710560801048352 76-77 23.885659457380836 26.073085955146368 25.85971946880519 24.181535118667608 78-79 23.94035445344205 26.194236304783665 25.794384149278137 24.071025092496146 80-81 23.81640401868217 26.158021870531527 26.12890098525662 23.896673125529684 82-83 23.995467587087475 25.704269391092353 26.116256017860845 24.18400700395933 84-85 23.760588988571918 25.9592158268278 26.086339691393285 24.193855493206993 86-87 23.802261352966323 26.35799715137475 26.019186219075337 23.82055527658359 88-89 24.123840280759396 26.200414418786984 25.7484739308182 23.927271369635424 90-91 24.09603918625793 25.911801052085316 25.786170566251883 24.20598919540487 92-93 23.827497890578915 26.454007153364245 25.948866139498072 23.769628816558768 94-95 24.156707697247327 25.997670329178007 25.765076591661717 24.080545381912945 96-97 24.214576123114142 26.079246142416064 25.670433714518254 24.03574401995154 98-99 24.170334778177256 26.024551146354852 25.918147911696515 23.88696616377138 100-101 24.662260557858183 25.864978902953588 25.310294611851685 24.162465927336545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.5 16 5.0 17 6.0 18 9.5 19 18.0 20 26.0 21 35.5 22 46.0 23 71.0 24 130.0 25 233.5 26 389.5 27 542.5 28 724.5 29 1063.0 30 1433.0 31 1683.5 32 1996.0 33 2367.5 34 2840.5 35 3318.0 36 3924.5 37 4721.0 38 5510.0 39 6259.0 40 7364.0 41 8736.0 42 9608.5 43 10256.0 44 10928.5 45 11153.5 46 11271.5 47 12042.0 48 12804.5 49 12874.0 50 12482.5 51 11758.0 52 10827.0 53 10433.0 54 10615.0 55 9955.0 56 8760.0 57 7986.5 58 6982.5 59 5802.5 60 4913.5 61 4177.5 62 3751.5 63 3282.0 64 2533.0 65 1954.0 66 1513.0 67 1247.5 68 1102.0 69 957.0 70 777.0 71 535.5 72 361.5 73 253.0 74 157.0 75 99.0 76 60.0 77 46.5 78 37.0 79 21.5 80 13.5 81 16.5 82 19.5 83 14.0 84 6.5 85 3.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11461681768459094 2 0.003733446830116969 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8667234150584845E-4 84-85 0.0 86-87 1.8667234150584845E-4 88-89 0.0014933787320467876 90-91 0.0 92-93 0.0011200340490350907 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.01456044263745618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267849.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.826611251945884 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66283073871745 52.122617512935754 2 9.894108596885154 12.630148461958491 3 3.18062640137576 6.090258145748586 4 1.5566170090070461 3.974143548082379 5 0.8538567368205473 2.7249391002950074 6 0.5533319107768025 2.119038045746843 7 0.3960736941041749 1.7696029190496514 8 0.2698969625036296 1.3781286803040145 9 0.23212846037984958 1.3334375701059644 >10 1.3969694370078172 15.730893862039416 >50 0.0035600524217624095 0.12679215373389083 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGG 60 0.0039460836 31.665857 9 CCCAGAT 105 6.861368E-5 27.157375 1 AATCAAC 70 0.008381328 27.142164 5 CTTGTAT 120 0.004739449 19.802252 1 TCGCTCA 130 0.007549489 18.268764 2 CCCCACT 195 0.0044338168 14.623201 1 TTCAACA 295 0.005166332 11.270898 2 CTTGAAG 305 0.006593838 10.90747 1 >>END_MODULE