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comparison w4mcorcov.xml @ 9:06c51af11531 draft

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planemo upload for repository https://github.com/HegemanLab/w4mcorcov_galaxy_wrapper/tree/master commit fda7ea61f0a082fd0c28730e471e66c92aaa04d0
author eschen42
date Fri, 10 Aug 2018 11:15:31 -0400
parents 342570ad880c
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8:342570ad880c 9:06c51af11531
1 <tool id="w4mcorcov" name="OPLS-DA_Contrasts" version="0.98.12"> 1 <tool id="w4mcorcov" name="OPLS-DA_Contrasts" version="0.98.13">
2 <description>OPLS-DA Contrasts of Univariate Results</description> 2 <description>OPLS-DA Contrasts of Univariate Results</description>
3 <macros> 3 <macros>
4 <xml name="paramPairSigFeatOnly"> 4 <xml name="paramPairSigFeatOnly">
5 <param name="pairSigFeatOnly" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE" 5 <param name="pairSigFeatOnly" type="boolean" checked="true" truevalue="TRUE" falsevalue="FALSE"
6 label="Retain only pairwise-significant features" 6 label="Retain only pairwise-significant features"
18 label="Produce orthogonal C-plot" 18 label="Produce orthogonal C-plot"
19 help="When this option is set to 'Yes', correlation will be plotted against vip4 for orthogonal loadings." /> 19 help="When this option is set to 'Yes', correlation will be plotted against vip4 for orthogonal loadings." />
20 </xml> 20 </xml>
21 </macros> 21 </macros>
22 <requirements> 22 <requirements>
23 <!--
24 <requirement type="package" version="3.4.1">r-base</requirement>
25 <requirement type="package" version="1.1_4">r-batch</requirement>
26 <requirement type="package" version="1.2.14">bioconductor-ropls</requirement>
27 -->
28 <requirement type="package">r-base</requirement>
23 <requirement type="package">r-batch</requirement> 29 <requirement type="package">r-batch</requirement>
24 <requirement type="package">bioconductor-ropls</requirement> 30 <requirement type="package" version="1.10.0">bioconductor-ropls</requirement>
25 </requirements> 31 </requirements>
26 <command detect_errors="aggressive"><![CDATA[ 32 <command detect_errors="aggressive"><![CDATA[
27 Rscript '$__tool_directory__/w4mcorcov_wrapper.R' 33 Rscript '$__tool_directory__/w4mcorcov_wrapper.R'
28 dataMatrix_in '$dataMatrix_in' 34 dataMatrix_in '$dataMatrix_in'
29 sampleMetadata_in '$sampleMetadata_in' 35 sampleMetadata_in '$sampleMetadata_in'
52 contrast_corcov '$contrast_corcov' 58 contrast_corcov '$contrast_corcov'
53 contrast_salience '$contrast_salience' 59 contrast_salience '$contrast_salience'
54 ]]></command> 60 ]]></command>
55 61
56 <inputs> 62 <inputs>
57 <param name="dataMatrix_in" type="data" format="tabular" label="Data matrix file" 63 <param name="dataMatrix_in" format="tabular" label="Data matrix file" type="data"
58 help="Features x samples (tabular data - decimal: '.'; missing: NA; mode: numerical; separator: tab character)" /> 64 help="variables &#10006; samples" />
59 <param name="sampleMetadata_in" type="data" format="tabular" label="Sample metadata file" 65 <param name="sampleMetadata_in" format="tabular" label="Sample metadata file" type="data"
60 help="Samples x metadata (tabular data - decimal: '.'; missing: NA; mode: character or numerical; separator: tab character)" /> 66 help="sample metadata, one row per sample" />
61 <param name="variableMetadata_in" type="data" format="tabular" 67 <param name="variableMetadata_in" format="tabular" label="Variable metadata file (ideally from Univariate)"
62 label="Variable metadata file (ideally from Univariate)" 68 type="data" help="variable metadata, one row per variable" />
63 help="Features x metadata (tabular data - decimal: '.'; missing: NA; mode: character or numerical; separator: tab character)" />
64 <param name="facC" type="text" 69 <param name="facC" type="text"
65 label="Factor of interest" 70 label="Factor of interest"
66 help="REQUIRED - The name of the column of sampleMetadata corresponding to the qualitative variable used to define the contrasts. Except when the 'Univariate Significance-test' is set to 'none', this also must be a portion of the column names in the variableMetadata file."/> 71 help="REQUIRED - The name of the column of sampleMetadata corresponding to the qualitative variable used to define the contrasts. Except when the 'Univariate Significance-test' is set to 'none', this also must be a portion of the column names in the variableMetadata file."/>
67 <conditional name="signif_test"> 72 <conditional name="signif_test">
68 <param name="tesC" label="Univariate significance-test" type="select" help="Either 'none' or the name of the statistical test that was run by the 'Univariate' tool to produce the variableMetadata file; that name must also be a portion of the column names in that file."> 73 <param name="tesC" label="Univariate significance-test" type="select" help="Either 'none' or the name of the statistical test that was run by the 'Univariate' tool to produce the variableMetadata file; that name must also be a portion of the column names in that file.">
147 * factor-level 2, lexically greater than level 1 152 * factor-level 2, lexically greater than level 1
148 * Wiklund_2008 correlation 153 * Wiklund_2008 correlation
149 * Wiklund_2008 covariance 154 * Wiklund_2008 covariance
150 * Galindo_Prieto_2014 VIP for predictive components, VIP[4,p] 155 * Galindo_Prieto_2014 VIP for predictive components, VIP[4,p]
151 * Galindo_Prieto_2014 VIP for orthogonal components, VIP[4,o] 156 * Galindo_Prieto_2014 VIP for orthogonal components, VIP[4,o]
152 * (When filtering on significance of univariate tests) Significance of test of null hypothesis that there is no difference between the two classes, i.e, the pair-wise test. 157 * When filtering on significance of univariate tests,significance of test of null hypothesis that there is no difference between the two classes, i.e, the pair-wise test.
153 --> 158 -->
154 <data name="contrast_corcov" format="tabular" label="${tool.name}_${variableMetadata_in.name}_corcov" /> 159 <data name="contrast_corcov" format="tabular" label="${tool.name}_${variableMetadata_in.name}_corcov" />
155 <!-- 160 <!--
156 tsv2: salience table with columns (experimental feature): 161 tsv2: salience table with columns (experimental feature):
157 * feature-ID 162 * feature-ID
422 Although this tool can be used in a purely exploratory manner by supplying the variableMetadata file without the columns added by the W4M 'Univariate' tool, **the preferred workflow may be to use univariate testing to exclude features that are not significantly different and then to use OPLS-DA to visualize the differences identified in univariate testing** (Th]]>&#233;<![CDATA[venot *et al.*, 2015); an appropriate exception would be to visualize contrasts of a specific list of metabolites. 427 Although this tool can be used in a purely exploratory manner by supplying the variableMetadata file without the columns added by the W4M 'Univariate' tool, **the preferred workflow may be to use univariate testing to exclude features that are not significantly different and then to use OPLS-DA to visualize the differences identified in univariate testing** (Th]]>&#233;<![CDATA[venot *et al.*, 2015); an appropriate exception would be to visualize contrasts of a specific list of metabolites.
423 428
424 It must be stressed that there may be no *single* definitive computational approach to select features that are reliable biomarkers, especially from a small number of samples or experiments. A few possible choices are: 429 It must be stressed that there may be no *single* definitive computational approach to select features that are reliable biomarkers, especially from a small number of samples or experiments. A few possible choices are:
425 430
426 - picking features with maximum loadings along the projection parallel to the predictor (loadp), 431 - picking features with maximum loadings along the projection parallel to the predictor (loadp),
427 - examining extreme values on S-PLOTs (for which covariance is linearly related to loadp), 432 - examining extreme values on S-PLOTs
428 - examining "variable importance in projection VIP for OPLS-DA" (Galindo-Prieto *et al.* 2014), and 433 - examining "variable importance in projection VIP for OPLS-DA" (Galindo-Prieto *et al.* 2014), and
429 - examining a feature's "selectivity ratio" (Rajalahti *et al.*, 2009). 434 - examining a feature's "selectivity ratio" (Rajalahti *et al.*, 2009).
430 435
431 In this spirit, this tool reports the S-PLOT covariance and correlation (Wiklund *op. cit.*) and VIP metrics, and it introduces an informal "salience" metric to flag features that may merit attention without dimensional reduction; future versions may add selectivity ratio. 436 In this spirit, this tool reports the S-PLOT covariance and correlation (Wiklund *op. cit.*) and VIP metrics, and it introduces an informal "salience" metric to flag features that may merit attention without dimensional reduction; future versions may add selectivity ratio.
432 437
598 Working examples 603 Working examples
599 ---------------- 604 ----------------
600 605
601 **Input files** 606 **Input files**
602 607
603 +-------------------------------------------------------------------------------------------------------------------+ 608 +-----------------------------------------------------------------------------------------------------------------------------------------------+
604 | Download from URL | 609 | Download from URL |
605 +===================================================================================================================+ 610 +===============================================================================================================================================+
606 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_dataMatrix.tsv | 611 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_dataMatrix.tsv |
607 +-------------------------------------------------------------------------------------------------------------------+ 612 +-----------------------------------------------------------------------------------------------------------------------------------------------+
608 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_sampleMetadata.tsv | 613 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_sampleMetadata.tsv |
609 +-------------------------------------------------------------------------------------------------------------------+ 614 +-----------------------------------------------------------------------------------------------------------------------------------------------+
610 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/input_variableMetadata.tsv | 615 | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/input_variableMetadata.tsv |
611 +-------------------------------------------------------------------------------------------------------------------+ 616 +-----------------------------------------------------------------------------------------------------------------------------------------------+
612 617
613 **Example 1:** Include in the analysis only features identified as pair-wise significant in the Univariate test. 618 **Example 1:** Include in the analysis only features identified as pair-wise significant in the Univariate test.
614 619
615 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 620 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
616 | Input Parameter or Result | Value | 621 | Input Parameter or Result | Value |
625 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 630 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
626 | Level-name matching | use regular expressions for matching level-names | 631 | Level-name matching | use regular expressions for matching level-names |
627 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 632 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
628 | Number of features having extreme loadings | ALL | 633 | Number of features having extreme loadings | ALL |
629 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 634 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
630 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov.tsv | 635 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov.tsv |
631 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 636 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
632 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience.tsv | 637 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience.tsv |
633 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 638 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
634 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail.pdf | 639 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail.pdf |
635 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 640 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+
636 641
637 **Example 2:** Include in the analysis only features identified as overall-significant in the Univariate test. Note that this even includes these features in contrasts where they were not determined to be pair-wise significant in the Univariate test. Thus, more features are included than in Example 1. 642 **Example 2:** Include in the analysis only features identified as overall-significant in the Univariate test. Note that this even includes these features in contrasts where they were not determined to be pair-wise significant in the Univariate test. Thus, more features are included than in Example 1.
638 643
639 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 644 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
640 | Input Parameter or Result | Value | 645 | Input Parameter or Result | Value |
641 +============================================+========================================================================================================================================+ 646 +============================================+============================================================================================================================================+
642 | Factor of interest | k10 | 647 | Factor of interest | k10 |
643 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 648 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
644 | Univariate Significance-Test | kruskal | 649 | Univariate Significance-Test | kruskal |
645 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 650 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
646 | Retain only pairwise-significant features | No | 651 | Retain only pairwise-significant features | No |
647 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 652 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
648 | Levels of interest | ``*`` | 653 | Levels of interest | ``*`` |
649 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 654 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
650 | Level-name matching | use wild cards for matching level-names | 655 | Level-name matching | use wild cards for matching level-names |
651 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 656 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
652 | Number of features having extreme loadings | 5 | 657 | Number of features having extreme loadings | 5 |
653 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 658 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
654 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_all.tsv | 659 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_all.tsv |
655 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 660 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
656 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_all.tsv | 661 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_all.tsv |
657 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 662 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
658 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_all.pdf | 663 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_all.pdf |
659 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 664 +--------------------------------------------+--------------------------------------------------------------------------------------------------------------------------------------------+
660 665
661 **Example 3:** Include all features in the analysis without regard to Univariate testing. Univariate testing is not even a pre-requisite to using the tool when 'none' is selected for the test. Thus, more features are included than in Example 2. 666 **Example 3:** Include all features in the analysis without regard to Univariate testing. Univariate testing is not even a pre-requisite to using the tool when 'none' is selected for the test. Thus, more features are included than in Example 2.
662 667
663 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 668 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
664 | Input Parameter or Result | Value | 669 | Input Parameter or Result | Value |
665 +============================================+========================================================================================================================================+ 670 +============================================+==============================================================================================================================================+
666 | Factor of interest | k10 | 671 | Factor of interest | k10 |
667 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 672 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
668 | Univariate Significance-Test | none | 673 | Univariate Significance-Test | none |
669 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 674 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
670 | Levels of interest | k[12],k[3-4] | 675 | Levels of interest | k[12],k[3-4] |
671 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 676 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
672 | Level-name matching | use regular expressions for matching level-names | 677 | Level-name matching | use regular expressions for matching level-names |
673 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 678 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
674 | Number of features having extreme loadings | 0 | 679 | Number of features having extreme loadings | 0 |
675 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 680 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
676 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_global.tsv | 681 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_global.tsv |
677 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 682 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
678 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_global.tsv | 683 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_global.tsv |
679 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 684 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
680 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_global.pdf | 685 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_global.pdf |
681 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 686 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
682 687
683 **Example 4:** Analysis of a two-level factor (including all features). This suppresses the contrasts of "each factor vs. the aggregate of all the others". 688 **Example 4:** Analysis of a two-level factor (including all features). This suppresses the contrasts of "each factor vs. the aggregate of all the others".
684 689
685 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 690 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
686 | Input Parameter or Result | Value | 691 | Input Parameter or Result | Value |
687 +============================================+========================================================================================================================================+ 692 +============================================+==============================================================================================================================================+
688 | Factor of interest | lohi | 693 | Factor of interest | lohi |
689 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 694 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
690 | Univariate Significance-Test | none | 695 | Univariate Significance-Test | none |
691 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 696 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
692 | Levels of interest | low,high | 697 | Levels of interest | low,high |
693 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 698 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
694 | Level-name matching | use regular expressions for matching level-names | 699 | Level-name matching | use regular expressions for matching level-names |
695 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 700 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
696 | Number of features having extreme loadings | 3 | 701 | Number of features having extreme loadings | 3 |
697 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 702 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
698 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_corcov_lohi.tsv | 703 | Output primary table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_corcov_lohi.tsv |
699 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 704 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
700 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_salience_lohi.tsv | 705 | Output salience table | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_salience_lohi.tsv |
701 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 706 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
702 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/test-data/expected_contrast_detail_lohi.pdf | 707 | Output figures PDF | https://raw.githubusercontent.com/HegemanLab/w4mcorcov_galaxy_wrapper/master/tools/w4mcorcov/test-data/expected_contrast_detail_lohi.pdf |
703 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------+ 708 +--------------------------------------------+----------------------------------------------------------------------------------------------------------------------------------------------+
704 709
705 710
706 Trademarks 711 Trademarks
707 ---------- 712 ----------
708 713