changeset 26:60da5215e182 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/qiime2-2020.8/qiime_vsearch_uchime-denovo.xml	Fri Sep 04 12:58:02 2020 +0000
@@ -0,0 +1,100 @@
+<?xml version="1.0" ?>
+<tool id="qiime_vsearch_uchime-denovo" name="qiime vsearch uchime-denovo"
+      version="2020.8">
+  <description>De novo chimera filtering with vsearch.</description>
+  <requirements>
+    <requirement type="package" version="2020.8">qiime2</requirement>
+  </requirements>
+  <command><![CDATA[
+qiime vsearch uchime-denovo
+
+--i-sequences=$isequences
+
+--i-table=$itable
+
+--p-dn=$pdn
+
+--p-mindiffs=$pmindiffs
+
+--p-mindiv=$pmindiv
+
+--p-minh=$pminh
+
+--p-xn=$pxn
+
+--o-chimeras=ochimeras
+
+--o-nonchimeras=ononchimeras
+
+--o-stats=ostats
+
+#if str($examples) != 'None':
+--examples=$examples
+#end if
+
+;
+cp ostats.qza $ostats
+
+  ]]></command>
+  <inputs>
+    <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT FeatureData[Sequence] The feature sequences to be chimera-checked. [required]" name="isequences" optional="False" type="data" />
+    <param format="qza,no_unzip.zip" label="--i-table: ARTIFACT FeatureTable[Frequency] Feature table (used for computing total feature abundances).                              [required]" name="itable" optional="False" type="data" />
+    <param label="--p-dn: NUMBER           No vote pseudo-count, corresponding to the Range(0.0, None)      parameter n in the chimera scoring function. [default: 1.4]" min="0.0" name="pdn" optional="True" type="float" value="1.4" />
+    <param label="--p-mindiffs: INTEGER    Minimum number of differences per segment. Range(1, None)                                                [default: 3]" min="1" name="pmindiffs" optional="True" type="integer" value="3" />
+    <param label="--p-mindiv: NUMBER       Minimum divergence from closest parent. Range(0.0, None)                                            [default: 0.8]" min="0.0" name="pmindiv" optional="True" type="float" value="0.8" />
+    <param exclude_max="False" label="--p-minh: PROPORTION Range(0.0, 1.0, inclusive_end=True) Minimum score (h). Increasing this value tends to reduce the number of false positives and to decrease sensitivity.                         [default: 0.28]" max="1.0" min="0.0" name="pminh" optional="True" type="float" value="0.28" />
+    <param exclude_min="True" label="--p-xn: NUMBER Range(1.0, None, inclusive_start=False) No vote weight, corresponding to the parameter beta in the scoring function.              [default: 8.0]" max="20.0" min="1.0" name="pxn" optional="True" type="float" value="8.0" />
+    <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" />
+    
+  </inputs>
+
+  <outputs>
+    <data format="qza" label="${tool.name} on ${on_string}: chimeras.qza" name="ochimeras" />
+    <data format="qza" label="${tool.name} on ${on_string}: nonchimeras.qza" name="ononchimeras" />
+    <data format="qza" label="${tool.name} on ${on_string}: stats.qza" name="ostats" />
+    
+  </outputs>
+
+  <help><![CDATA[
+De novo chimera filtering with vsearch.
+###############################################################
+
+Apply the vsearch uchime_denovo method to identify chimeric feature
+sequences. The results of this method can be used to filter chimeric
+features from the corresponding feature table. For additional details,
+please refer to the vsearch documentation.
+
+Parameters
+----------
+sequences : FeatureData[Sequence]
+    The feature sequences to be chimera-checked.
+table : FeatureTable[Frequency]
+    Feature table (used for computing total feature abundances).
+dn : Float % Range(0.0, None), optional
+    No vote pseudo-count, corresponding to the parameter n in the chimera
+    scoring function.
+mindiffs : Int % Range(1, None), optional
+    Minimum number of differences per segment.
+mindiv : Float % Range(0.0, None), optional
+    Minimum divergence from closest parent.
+minh : Float % Range(0.0, 1.0, inclusive_end=True), optional
+    Minimum score (h). Increasing this value tends to reduce the number of
+    false positives and to decrease sensitivity.
+xn : Float % Range(1.0, None, inclusive_start=False), optional
+    No vote weight, corresponding to the parameter beta in the scoring
+    function.
+
+Returns
+-------
+chimeras : FeatureData[Sequence]
+    The chimeric sequences.
+nonchimeras : FeatureData[Sequence]
+    The non-chimeric sequences.
+stats : UchimeStats
+    Summary statistics from chimera checking.
+  ]]></help>
+  <macros>
+    <import>qiime_citation.xml</import>
+  </macros>
+  <expand macro="qiime_citation"/>
+</tool>
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