Mercurial > repos > frogs > frogs_3_1_0
diff r_alpha_diversity.xml @ 0:59bc96331073 draft default tip
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
author | frogs |
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date | Thu, 28 Feb 2019 10:14:49 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/r_alpha_diversity.xml Thu Feb 28 10:14:49 2019 -0500 @@ -0,0 +1,127 @@ +<?xml version="1.0" encoding="UTF-8"?> +<!-- +# Copyright (C) 2017 INRA +# +# This program is free software: you can redistribute it and/or modify +# it under the terms of the GNU General Public License as published by +# the Free Software Foundation, either version 3 of the License, or +# (at your option) any later version. +# +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. +# +# You should have received a copy of the GNU General Public License +# along with this program. If not, see <http://www.gnu.org/licenses/>. +--> +<tool name="FROGSSTAT Phyloseq Alpha Diversity" id="FROGSSTAT_Phyloseq_Alpha_Diversity" version="3.1"> + <description>with richness plot</description> + <requirements> + <requirement type="package" version="3.1.0">frogs</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + <exit_code range=":-1" /> + </stdio> + <command>r_alpha_diversity.py + --rdata $data + --varExp $varExp + --alpha-measures $measures + --alpha-out $alphaD + --html $html + </command> + <inputs> + <!-- Files --> + <param format="rdata" name="data" type="data" label="Phyloseq object (format rdata)" help="This file is the result of FROGS Phyloseq Import Data tool." optional="false"> + <validator type="empty_field" message="This parameter is required." /> + </param> + <!-- Parameters --> + <param name="varExp" type="text" label="Experiment variable" help="The experiment variable that you want to analyse." optional="false" value="" size="40"> + <validator type="empty_field" message="This parameter is required." /> + </param> + <param name="measures" type="select" label="The alpha diversity indices to compute" multiple="true" display="checkboxes"> + <option value="Observed" selected="True">Observed</option> + <option value="Chao1" selected="True">Chao1</option> + <option value="Shannon" selected="True">Shannon</option> + <option value="InvSimpson" selected="True">InvSimpson</option> + <option value="Simpson">Simpson</option> + <option value="ACE">ACE</option> + <option value="Fisher">Fisher</option> + </param> + </inputs> + <outputs> + <data format="tabular" name="alphaD" label="${tool.name}: alpha_diversity.tsv" from_work_dir="alpha_diversity.tsv"/> + <data format="html" name="html" label="${tool.name}: alpha_diversity.html" from_work_dir="alpha_diversity.html"/> + </outputs> + <tests> + <test> + <param name="data" value="references/14-phylo_import.Rdata" /> + <param name="varExp" value="EnvType" /> + <param name="measures" value="Observed,Chao1,Shannon" /> + <output name="alphaD" file="references/16-phylo_alpha_div.tsv" compare="diff" lines_diff="0" /> + </test> + </tests> +<help> + +.. image:: static/images/frogs_images/FROGS_logo.png + :height: 144 + :width: 110 + +.. class:: infomark page-header h2 + +What it does + +Using `phyloseq <https://joey711.github.io/phyloseq/>`_ and customized R function, this tool constructs richness plots to visualise sample alpha diversity. + +.. class:: infomark page-header h2 + +Inputs/Outputs + +.. class:: h3 + +Input + +**Data file** (format RData): +One phyloseq object containing OTU abundance table, their taxonomies (and optionnaly a phylogenetics tree, and the sample experiment metadata. +This file is the result of "FROGS Phyloseq Import Data tool". + +.. class:: h3 + +Output + +**Html file** (format `HTML <https://en.wikipedia.org/wiki/HTML>`_): the review of richness plots and rarefaction curves. + +.. image:: static/images/frogs_images/phyloseq_plot_richness.png + :height: 400 + :width: 525 + +.. image:: static/images/frogs_images/phyloseq_plot_richness_box.png + :height: 400 + :width: 499 + +.. image:: static/images/frogs_images/phyloseq_rarefaction_curves.png + :height: 400 + :width: 498 + +**Alpha diversity table** (alpha_diversity.tsv): +The data file containing table of alpha diversity indices. + +.. image:: static/images/frogs_images/phyloseq_alpha_diversity_table.png + +.. class:: infomark page-header h2 + +**Contact** + +Contacts: frogs@inra.fr + +Repository: https://github.com/geraldinepascal/FROGS +website: http://frogs.toulouse.inra.fr/ + +Please cite the **FROGS article**: *Escudie F., et al. Bioinformatics, 2018. FROGS: Find, Rapidly, OTUs with Galaxy Solution.* + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btx791</citation> + </citations> +</tool>