Mercurial > repos > frogs > frogs_3_1_0
diff tree.xml @ 0:59bc96331073 draft default tip
planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
author | frogs |
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date | Thu, 28 Feb 2019 10:14:49 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tree.xml Thu Feb 28 10:14:49 2019 -0500 @@ -0,0 +1,126 @@ +<?xml version="1.0"?> +<!-- +# Copyright (C) 2017 INRA +# +# This program is free software: you can redistribute it and/or modify +# it under the terms of the GNU General Public License as published by +# the Free Software Foundation, either version 3 of the License, or +# (at your option) any later version. +# +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. +# +# You should have received a copy of the GNU General Public License +# along with this program. If not, see <http://www.gnu.org/licenses/>. +--> +<tool id="FROGS_Tree" name="FROGS Tree" version="3.1"> + <description>Reconstruction of phylogenetic tree </description> + <requirements> + <requirement type="package" version="3.1.0">frogs</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + <exit_code range=":-1" /> + </stdio> + <command>tree.py + --input-otu $input_otu + --biomfile $biomfile + --nb-cpus \${GALAXY_SLOTS:-1} + --out-tree $out_tree + --html $html + </command> + <inputs> + <!-- Files --> + <param format="fasta" name="input_otu" type="data" label="OTUs sequence file" help="OTUs sequence file (format: fasta). Warning: FROGS Tree does not work on more than 10000 sequences!" optional="false"> + <validator type="empty_field" message="This parameter is required." /> + </param> + <param format="biom1" name="biomfile" type="data" label="Biom file" help="The abundance table of OTUs (format: biom)." optional="false"> + <validator type="empty_field" message="This parameter is required." /> + </param> + </inputs> + <outputs> + <data format="nhx" name="out_tree" label="${tool.name}: tree.nwk" from_work_dir="tree.nwk"/> + <data format="html" name="html" label="${tool.name}: summary.html" from_work_dir="summary.html"/> + </outputs> + <tests> + <test> + <param name="input_otu" value="references/04-filters.fasta"/> + <param name="biomfile" value="references/06-affiliation.biom"/> + <!--output name="out_tree" value="references/13-tree-mafft.nwk"/--> + <!--output name="html" value="references/13-tree-mafft.html"/--> + <output name="html"> + <assert_contents> + <has_text_matching expression="FROGS\sTree" /> + <has_text_matching expression="abundance_removed.*:\s0" /> + <has_text_matching expression="abundance_kept.*:\s45574" /> + <has_text_matching expression="otu_removed.*:\s0" /> + <has_text_matching expression="otu_kept.*:\s86" /> + </assert_contents> + </output> + </test> + </tests> + <help> +.. image:: static/images/frogs_images/FROGS_logo.png + :height: 144 + :width: 110 + +.. class:: infomark page-header h2 + +What it does + +Creation of a multiple alignment of OTUs with `Mafft <http://mafft.cbrc.jp/alignment/software>`_. +And creation of a rooted phylogenetic tree with `FastTree <http://www.microbesonline.org/fasttree/>`_ and `Phangorn R package <https://cran.r-project.org/web/packages/phangorn/index.html>`_. + +.. class:: infomark page-header h2 + +Inputs/Outputs + +.. class:: h3 + +Input + +**OTUs fasta file**: + +The OTUs sequence file (format `FASTA <https://en.wikipedia.org/wiki/FASTA_format>`_). +Careful: FROGS Tree works only with less than 10 000 sequences! + + .. image:: static/images/frogs_images/frogs_tree_otufile.png + +**OTUs biom file**: + +The OTUs biom file (format `biom1 <http://biom-format.org/documentation/format_versions/biom-1.0.html>`_). +This file can be obtained in particular with the FROGS pipeline. + +.. class:: h3 + +Outputs + +**Newick file** (tree.nwk): + +The phylogenetic tree in Newick format (format `nxh <https://en.wikipedia.org/wiki/Newick_format>`_). + + .. image:: static/images/frogs_images/nwk_treefile.png + +**Html file** (summary.html): + +The summary file describing which OTUs are contained or not in the phylogenetic tree. + +.. class:: infomark page-header h2 + +**Contact** + +Contacts: frogs@inra.fr + +Repository: https://github.com/geraldinepascal/FROGS +website: http://frogs.toulouse.inra.fr/ + +Please cite the **FROGS article**: *Escudie F., et al. Bioinformatics, 2018. FROGS: Find, Rapidly, OTUs with Galaxy Solution.* + + </help> + <citations> + <citation type="doi">10.1093/bioinformatics/btx791</citation> + </citations> + +</tool>