comparison vcfutils_vcf2fq.xml @ 0:921286a54e95 draft default tip

Uploaded wrapper for vcutils.pl vcf2fq
author ftouzain
date Thu, 22 Jun 2023 15:41:59 +0000
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1 <tool id="vcfutils_vcf2fq" name="vcfutils_vcf2fq: convert vcf file to fastq file" version="1.16" python_template_version="3.9">
2 <requirements>
3 <requirement type="package" version="1.16">bcftools</requirement>
4 </requirements>
5 <command detect_errors="exit_code"><![CDATA[
6 vcfutils.pl vcf2fq -d '$min_depth' -D '$max_depth' -Q '$min_RMS_mapQ' -l '$filtering_window' '$all_sites_vcf_f' > '$fastq_f'
7 ]]></command>
8 <inputs>
9 <param type="data" name="all_sites_vcf_f" format="vcf" />
10 <param type="integer" name="min_depth" label="min covereage depth" value="3" />
11 <param type="integer" name="max_depth" label="max coverage depth" value="100000" />
12 <param type="integer" name="min_RMS_mapQ" label="min RMS mapQ" value="10" />
13 <param type="integer" name="filtering_window" label="filtering window" value="5" />
14 </inputs>
15 <outputs>
16 <data name="fastq_f" format="fastq" />
17 </outputs>
18 <tests>
19 <test>
20 <param name="all_sites_vcf_f" value="test_vcfutils_vcf2fq/all_sites.vcf"/>
21 <output name="fastq_f" file="test_vcfutils_vcf2fq/consensus.fastq"/>
22 </test>
23 </tests>
24 <help><![CDATA[
25 [vcfutils_vcf2fq]
26 Aim: Deduce from vcf file output fastq file
27 in:
28 - vcf file with all sites
29 out:
30 - consensus fastq file
31
32 usage: vcfutils.pl vcf2fq all_sites.vcf > consensus.fastq
33
34 optional arguments:
35 -d INT minimum depth [3]
36 -D INT maximum depth [100000]
37 -Q INT min RMS mapQ [10]
38 -l INT INDEL filtering window [5]
39 ]]></help>
40 <citations>
41 <citation type="bibtex">
42 @misc{vcfutils_vcf2fq,
43 author = {Li, Heng},
44 year = {2009},
45 title = {The Sequence Alignment/Map format and SAMtools},
46 publisher = {},
47 journal = {Bioinformatics},
48 url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2723002},
49 }</citation>
50 <citation type="doi">10.1093/bioinformatics/btp352</citation>
51 </citations>
52 </tool>