Mercurial > repos > fubar > bigwig_outlier_bed
diff README.md @ 6:eb17eb8a3658 draft
planemo upload commit 1baff96e75def9248afdcf21edec9bdc7ed42b1f-dirty
author | fubar |
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date | Tue, 23 Jul 2024 23:12:23 +0000 |
parents | c71db540eb38 |
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--- a/README.md Mon Jul 01 05:02:05 2024 +0000 +++ b/README.md Tue Jul 23 23:12:23 2024 +0000 @@ -1,10 +1,10 @@ -## bigwig peak outlier to bed +## bigwig peak bed maker ### July 30 2024 for the VGP -This code will soon become a Galaxy tool, for building some of the [NIH MARBL T2T assembly polishing](https://github.com/marbl/training) tools as Galaxy workflows. +This is a Galaxy tool, for building some of the [NIH MARBL T2T assembly polishing](https://github.com/marbl/training) tools as Galaxy workflows. -The next JBrowse2 tool release will include a plugin for optional colours to distinguish bed features, shown being tested in the screenshots below. +JBrowse2 2.12.3 update will include a plugin for optional colours to distinguish bed features, shown being tested in the screenshots below. ### Find and mark BigWig peaks to a bed file for display @@ -29,3 +29,69 @@ better to sample across all chromosomes so as not to lose any systematic differences between them - the current method will hide those differences unfortunately. Sampling might be possible. Looking at the actual quantile values across a couple of test bigwigs suggests that there is not much variation between chromosomes but there's now a tabular report to check them for each input bigwig. + +### Table reports + +The optional table output report gives a crude histogram and the top/bottom 10 values to help +understand what is likely to be informative. In this example, there are 26700 zero values so +using a lower cutoff quantile is likely to have a lot of them, although a large window requirement +will decease the overload... + +Descriptive measures +bigwig test +contig chr10_PATERNAL +n 135711693 +mean 12.178164 +std 7.997467 +min 0.000000 +max 365.000000 +qtop 364.00 +qbot noqlo +First/Last 10 value counts +Value Count +0.00 26700 +1.00 82900 +2.00 261400 +3.00 676993 +4.00 1665500 +5.00 3125700 +6.00 5078000 +7.00 7469000 +8.00 10191700 +9.00 12544600 +355.00 100 +356.00 100 +357.00 300 +358.00 100 +360.00 500 +361.00 300 +362.00 200 +363.00 600 +364.00 900 +365.00 700 +Histogram of bigwig values +chr10_PATERNAL 18.25 | 127,047,593 | ************************************************************************** +chr10_PATERNAL 36.50 | 7,510,000 | **** +chr10_PATERNAL 54.75 | 818,900 | +chr10_PATERNAL 73.00 | 117,200 | +chr10_PATERNAL 91.25 | 51,900 | +chr10_PATERNAL 109.50 | 44,200 | +chr10_PATERNAL 127.75 | 21,600 | +chr10_PATERNAL 146.00 | 17,900 | +chr10_PATERNAL 164.25 | 16,400 | +chr10_PATERNAL 182.50 | 18,600 | +chr10_PATERNAL 200.75 | 5,400 | +chr10_PATERNAL 219.00 | 6,600 | +chr10_PATERNAL 237.25 | 6,200 | +chr10_PATERNAL 255.50 | 3,900 | +chr10_PATERNAL 273.75 | 4,500 | +chr10_PATERNAL 292.00 | 7,100 | +chr10_PATERNAL 310.25 | 3,000 | +chr10_PATERNAL 328.50 | 2,700 | +chr10_PATERNAL 346.75 | 3,500 | +chr10_PATERNAL 365.00 | 4,500 | +chr10_PATERNAL ------------ |------------ | +chr10_PATERNAL N= | 135,711,693 | +chr10_PATERNAL ------------ |------------ | + +