annotate readme.rst @ 50:f350467f9433 draft

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date Thu, 07 Mar 2024 04:51:43 +0000
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1 JBrowse2 in Galaxy
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2 ==================
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3
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4 Added Mar 3:
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5 - optional tracks at last for JB2
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6 - dotted noodles from optional inputs now ignored without harm
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7 - autogenJB2.
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8 - Takes a collection of bam/vcf etc and turns them into tracks in a normal JB2 history item.
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9 - Uses the JbrowseConnector, but the XML tool drives it from the contents of the collection.
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10 - Collection can be built from any source or with optional noodles in a WF
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11 - Produces a JB2 with all available tracks.
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12 - Argparse command line with repeats for tracks and references
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13 - so works outside Galaxy to process directories or s3 buckets.
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16 Added Feb 3: cool/mcool -> hic
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18 .. image:: dm4_in_jb2.png
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20 Other tracks
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22 .. image:: jbrowse8.png
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24 JBrowse2 is a fast, embeddable genome browser built completely with
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25 JavaScript and HTML5
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27 Thus, it makes an ideal fit with Galaxy, especially for use as a
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28 workflow summary. E.g. annotate a genome, then visualise all of the
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29 associated datasets as an interactive HTML page. This tool MUST be whitelisted
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30 (or ``sanitize_all_html=False`` in galaxy.ini) to function correctly.
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31 The built-in Galaxy gunicorn server does not support byte range requests, so this tool must be proxied by nginx
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32 or another web server, correctly configured to support range requests. A tiny web server is bundled
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33 with each JBrowse2 archive - see below.
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35 Installation
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36 ============
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37
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38 This wrapper is normally installed by a server administrator from the Galaxy Tool Shed IUC JBrowse2 repository.
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40 Local display
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41 =============
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42
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43 Each JBrowse2 history item can be downloaded ("floppy disk" icon) to your local disk. There, it can be unzipped into a new directory.
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44 That directory includes a python script, *jb2_webserver.py* that will run a local web server able to serve byte range requests,
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45 giving the same view as seen when viewed from the Galaxy history.
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47 From the newly unzipped directory where that file can be found, and with Python3 installed and working,
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49 `python3 jb2_webserver.py`
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51 will open the preconfigured browser using the default web browser application.
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52
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53 That webserver code is separately under the Apache 2 license copied in this repository. See below for this code's MIT license.
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54
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55 History
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56 =======
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58 - 2.10.0+galaxy2
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59
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60 - UPDATED existing JBrowse1.16.11 code to JBrowse 2.10.0
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61 - was working well enough for VGP when previous PR discovered
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62 - too late to backport all the fixes
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63 - working default session and some other ideas copied instead.
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64 - seems to work well with defaults.
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65 - need to document and implement track settings by running the browser locally.
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66 - works well enough to be useful in workflows such as TreeValGal.
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67 - JB2 seems to set defaults wisely.
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68 - not yet ideal for users who need fine grained track control.
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69 - synteny (paf + reference) now working
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70 - rehomed at https://github.com/usegalaxy-eu/temporary-tools/tree/master/jbrowse2 while IUC reviews are slowly sorted out.
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73 Wrapper License (MIT/BSD Style)
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74 ===============================
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75
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76 Permission to use, copy, modify, and distribute this software and its
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77 documentation with or without modifications and for any purpose and
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78 without fee is hereby granted, provided that any copyright notices
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79 appear in all copies and that both those copyright notices and this
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80 permission notice appear in supporting documentation, and that the names
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81 of the contributors or copyright holders not be used in advertising or
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82 publicity pertaining to distribution of the software without specific
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83 prior permission.
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84
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85 THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL
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86 WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED
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87 WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE
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88 CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT OR
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89 CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS OF
d78175596286 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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90 USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE OR
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91 OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE OR
d78175596286 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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92 PERFORMANCE OF THIS SOFTWARE.