Mercurial > repos > fubar > jbrowse2
annotate blastxml_to_gapped_gff3.py @ 128:fce4ed3b1702 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit a46026a5ea4f075b9053448616f22e8a83fa699a
author | bgruening |
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date | Mon, 07 Oct 2024 06:30:43 +0000 |
parents | b1260bca5fdc |
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rev | line source |
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d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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1 #!/usr/bin/env python |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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2 import argparse |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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3 import copy |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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4 import logging |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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5 import re |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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6 import sys |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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7 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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8 from BCBio import GFF |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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9 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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10 logging.basicConfig(level=logging.INFO) |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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11 log = logging.getLogger(name="blastxml2gff3") |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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12 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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13 __doc__ = """ |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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14 BlastXML files, when transformed to GFF3, do not normally show gaps in the |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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15 blast hits. This tool aims to fill that "gap". |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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16 """ |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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17 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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18 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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19 def blastxml2gff3(blastxml, min_gap=3, trim=False, trim_end=False, include_seq=False): |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
diff
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20 from Bio.Blast import NCBIXML |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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21 from Bio.Seq import Seq |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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22 from Bio.SeqRecord import SeqRecord |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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23 from Bio.SeqFeature import SeqFeature, SimpleLocation |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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24 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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25 blast_records = NCBIXML.parse(blastxml) |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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26 for idx_record, record in enumerate(blast_records): |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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27 # http://www.sequenceontology.org/browser/release_2.4/term/SO:0000343 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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28 match_type = { # Currently we can only handle BLASTN, BLASTP |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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29 "BLASTN": "nucleotide_match", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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30 "BLASTP": "protein_match", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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31 }.get(record.application, "match") |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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32 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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33 recid = record.query |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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34 if " " in recid: |
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b1260bca5fdc
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit 44d8fc559ecf5463a8f753561976fa26686c96f6
bgruening
parents:
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35 recid = recid[0: recid.index(" ")] |
0
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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36 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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37 rec = SeqRecord(Seq("ACTG"), id=recid) |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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38 for idx_hit, hit in enumerate(record.alignments): |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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39 for idx_hsp, hsp in enumerate(hit.hsps): |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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40 qualifiers = { |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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41 "ID": "b2g.%s.%s.%s" % (idx_record, idx_hit, idx_hsp), |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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42 "source": "blast", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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43 "score": hsp.expect, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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44 "accession": hit.accession, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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45 "hit_id": hit.hit_id, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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46 "length": hit.length, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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47 "hit_titles": hit.title.split(" >"), |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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48 } |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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49 if include_seq: |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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50 qualifiers.update( |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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51 { |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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52 "blast_qseq": hsp.query, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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53 "blast_sseq": hsp.sbjct, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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54 "blast_mseq": hsp.match, |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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55 } |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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56 ) |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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57 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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58 for prop in ( |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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59 "score", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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60 "bits", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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61 "identities", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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62 "positives", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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63 "gaps", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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64 "align_length", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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65 "strand", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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66 "frame", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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67 "query_start", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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68 "query_end", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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69 "sbjct_start", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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70 "sbjct_end", |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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71 ): |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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72 qualifiers["blast_" + prop] = getattr(hsp, prop, None) |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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73 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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74 desc = hit.title.split(" >")[0] |
98
b1260bca5fdc
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit 44d8fc559ecf5463a8f753561976fa26686c96f6
bgruening
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75 qualifiers["description"] = desc[desc.index(" "):] |
0
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
parents:
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76 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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77 # This required a fair bit of sketching out/match to figure out |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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78 # the first time. |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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79 # |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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80 # the match_start location must account for queries and |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
fubar
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81 # subjecst that start at locations other than 1 |
d78175596286
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/jbrowse2 commit cd77dffaad652cfb75b98bde5231beaa6d63cd5b-dirty
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82 parent_match_start = hsp.query_start - hsp.sbjct_start |
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83 # The end is the start + hit.length because the match itself |
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84 # may be longer than the parent feature, so we use the supplied |
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85 # subject/hit length to calculate the real ending of the target |
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86 # protein. |
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87 parent_match_end = hsp.query_start + hit.length + hsp.query.count("-") |
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88 |
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89 # If we trim the left end, we need to trim without losing information. |
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90 used_parent_match_start = parent_match_start |
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91 if trim: |
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92 if parent_match_start < 1: |
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93 used_parent_match_start = 0 |
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94 |
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95 if trim or trim_end: |
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96 if parent_match_end > hsp.query_end: |
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97 parent_match_end = hsp.query_end + 1 |
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98 |
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99 # The ``match`` feature will hold one or more ``match_part``s |
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100 top_feature = SeqFeature( |
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101 SimpleLocation(used_parent_match_start, parent_match_end, strand=0), |
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102 type=match_type, |
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103 qualifiers=qualifiers, |
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104 ) |
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105 |
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106 # Unlike the parent feature, ``match_part``s have sources. |
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107 part_qualifiers = { |
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108 "source": "blast", |
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109 } |
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110 top_feature.sub_features = [] |
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111 for idx_part, (start, end, cigar) in enumerate( |
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112 generate_parts( |
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113 hsp.query, hsp.match, hsp.sbjct, ignore_under=min_gap |
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114 ) |
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115 ): |
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116 part_qualifiers["Gap"] = cigar |
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117 part_qualifiers["ID"] = qualifiers["ID"] + (".%s" % idx_part) |
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118 |
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119 # Otherwise, we have to account for the subject start's location |
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120 match_part_start = parent_match_start + hsp.sbjct_start + start - 1 |
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121 |
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122 # We used to use hsp.align_length here, but that includes |
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123 # gaps in the parent sequence |
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124 # |
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125 # Furthermore align_length will give calculation errors in weird places |
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126 # So we just use (end-start) for simplicity |
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127 match_part_end = match_part_start + (end - start) |
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128 |
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129 top_feature.sub_features.append( |
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130 SeqFeature( |
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131 SimpleLocation(match_part_start, match_part_end, strand=1), |
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132 type="match_part", |
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133 qualifiers=copy.deepcopy(part_qualifiers), |
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134 ) |
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135 ) |
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136 |
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137 rec.features.append(top_feature) |
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138 rec.annotations = {} |
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139 yield rec |
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140 |
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141 |
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142 def __remove_query_gaps(query, match, subject): |
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143 """remove positions in all three based on gaps in query |
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144 |
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145 In order to simplify math and calculations...we remove all of the gaps |
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146 based on gap locations in the query sequence:: |
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147 |
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148 Q:ACTG-ACTGACTG |
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149 S:ACTGAAC---CTG |
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150 |
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151 will become:: |
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152 |
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153 Q:ACTGACTGACTG |
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154 S:ACTGAC---CTG |
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155 |
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156 which greatly simplifies the process of identifying the correct location |
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157 for a match_part |
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158 """ |
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159 prev = 0 |
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160 fq = "" |
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161 fm = "" |
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162 fs = "" |
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163 for position in re.finditer("-", query): |
98
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164 fq += query[prev: position.start()] |
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165 fm += match[prev: position.start()] |
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166 fs += subject[prev: position.start()] |
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167 prev = position.start() + 1 |
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168 fq += query[prev:] |
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169 fm += match[prev:] |
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170 fs += subject[prev:] |
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171 |
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172 return (fq, fm, fs) |
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173 |
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174 |
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175 def generate_parts(query, match, subject, ignore_under=3): |
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176 region_q = [] |
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177 region_m = [] |
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178 region_s = [] |
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179 |
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180 (query, match, subject) = __remove_query_gaps(query, match, subject) |
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181 |
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182 region_start = -1 |
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183 region_end = -1 |
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184 mismatch_count = 0 |
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185 for i, (q, m, s) in enumerate(zip(query, match, subject)): |
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186 |
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187 # If we have a match |
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188 if m != " " or m == "+": |
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189 if region_start == -1: |
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190 region_start = i |
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191 # It's a new region, we need to reset or it's pre-seeded with |
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192 # spaces |
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193 region_q = [] |
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194 region_m = [] |
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195 region_s = [] |
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196 region_end = i |
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197 mismatch_count = 0 |
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198 else: |
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199 mismatch_count += 1 |
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200 |
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201 region_q.append(q) |
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202 region_m.append(m) |
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203 region_s.append(s) |
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204 |
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205 if mismatch_count >= ignore_under and region_start != -1 and region_end != -1: |
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206 region_q = region_q[0:-ignore_under] |
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207 region_m = region_m[0:-ignore_under] |
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208 region_s = region_s[0:-ignore_under] |
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209 yield region_start, region_end + 1, cigar_from_string( |
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210 region_q, region_m, region_s, strict_m=True |
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211 ) |
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212 region_q = [] |
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213 region_m = [] |
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214 region_s = [] |
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215 |
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216 region_start = -1 |
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217 region_end = -1 |
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218 mismatch_count = 0 |
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219 |
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220 yield region_start, region_end + 1, cigar_from_string( |
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221 region_q, region_m, region_s, strict_m=True |
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222 ) |
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223 |
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224 |
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225 def _qms_to_matches(query, match, subject, strict_m=True): |
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226 matchline = [] |
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227 |
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228 for (q, m, s) in zip(query, match, subject): |
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229 ret = "" |
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230 |
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231 if m != " " or m == "+": |
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232 ret = "=" |
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233 elif m == " ": |
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234 if q == "-": |
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235 ret = "D" |
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236 elif s == "-": |
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237 ret = "I" |
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238 else: |
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239 ret = "X" |
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240 else: |
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241 log.warn("Bad data: \n\t%s\n\t%s\n\t%s\n" % (query, match, subject)) |
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242 |
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243 if strict_m: |
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244 if ret == "=" or ret == "X": |
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245 ret = "M" |
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246 |
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247 matchline.append(ret) |
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248 return matchline |
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249 |
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250 |
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251 def _matchline_to_cigar(matchline): |
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252 cigar_line = [] |
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253 last_char = matchline[0] |
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254 count = 0 |
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255 for char in matchline: |
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256 if char == last_char: |
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257 count += 1 |
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258 else: |
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259 cigar_line.append("%s%s" % (last_char, count)) |
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260 count = 1 |
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261 last_char = char |
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262 cigar_line.append("%s%s" % (last_char, count)) |
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263 return " ".join(cigar_line) |
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264 |
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265 |
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266 def cigar_from_string(query, match, subject, strict_m=True): |
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267 matchline = _qms_to_matches(query, match, subject, strict_m=strict_m) |
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268 if len(matchline) > 0: |
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269 return _matchline_to_cigar(matchline) |
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270 else: |
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271 return "" |
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272 |
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273 |
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274 if __name__ == "__main__": |
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275 parser = argparse.ArgumentParser( |
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276 description="Convert Blast XML to gapped GFF3", epilog="" |
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277 ) |
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278 parser.add_argument( |
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279 "blastxml", type=argparse.FileType("r"), help="Blast XML Output" |
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280 ) |
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281 parser.add_argument( |
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282 "--min_gap", |
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283 type=int, |
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284 help="Maximum gap size before generating a new match_part", |
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285 default=3, |
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286 ) |
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287 parser.add_argument( |
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288 "--trim", |
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289 action="store_true", |
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290 help="Trim blast hits to be only as long as the parent feature", |
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291 ) |
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292 parser.add_argument( |
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293 "--trim_end", |
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294 action="store_true", |
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295 help="Cut blast results off at end of gene", |
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296 ) |
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297 parser.add_argument("--include_seq", action="store_true", help="Include sequence") |
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298 args = parser.parse_args() |
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299 |
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300 for rec in blastxml2gff3(**vars(args)): |
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301 if len(rec.features): |
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302 GFF.write([rec], sys.stdout) |