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1 <tool id="multiplicom_primer_trimming" name="Multiplicom Primer Trimmer" version="0.0.1">
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2 <description></description>
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3 <requirements>
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4 <requirement type="package" version='latest'>twoBitToFa</requirement>
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5 </requirements>
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6 <command interpreter="perl">
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7 multiplicom_primer_trimming.pl
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8 ## input files
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9 -i $inputf
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10 -I $inputr
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11 -b $mastr
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12 ## read length
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13 -r $readlength
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14 ## output files
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15 -o $outf
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16 -O $outr
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17 -F $failed
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18 -R $report
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19 ## reference genome
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20 -t "${indexes.fields.path}"
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21
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22 </command>
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23 <inputs>
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24 <param name='inputf' type='data' format='fastq,fastqsanger' label='Forward Sequences' help='Forward Reads in fastq format' />
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25 <param name='inputr' type='data' format='fastq,fastqsanger' label='Reverse Sequences' help='Reverse Reads in fastq format' />
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26 <param name='mastr' type='data' format='bed' label='MASTR file' help='Design file of the Multiplicom MASTR assay' />
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27 <param name='readlength' type='integer' value='250' label='Read Length' help='Applied Readlength, per read' />
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28 <param name="indexes" type="select" label="Reference Genome" help="Select the correct genome build" >
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29 <options from_data_table="2bit" >
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30 <filter type="sort_by" column="2" />
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31 <validator type="no_options" message="No indexes are available" />
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32 </options>
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33 </param>
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34 </inputs>
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35 <outputs>
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36 <data format_source="inputf" name="outf" label="${tool.name} on ${on_string}: Forward Reads"/>
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37 <data format_source="inputr" name="outr" label="${tool.name} on ${on_string}: Reverse Reads"/>
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38 <data format_source="inputf" name="failed" label="${tool.name} on ${on_string}: Failed Pairs"/>
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39 <data format="txt" name="report" label="${tool.name} on ${on_string}: Runtime output"/>
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40 </outputs>
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41 <help>
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42 This tools scans paired FASTQ files for the presence of Multiplicom MASTR PCR primers. If found, primers are clipped.
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43 </help>
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44 </tool>
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45
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